Recombinant Human CFAP92 Protein: Research Guide & Protocol

Recombinant Human CFAP92 Protein is a research-grade reagent designed to support investigations into cilia structure, function, and associated signaling pathways in human biology.

Product Name

Recombinant Human CFAP92 Protein

SKU

rp376081

Supplier Reference

absea#PP-9701

Species Origin

Human (Homo sapiens)

Protein Family

Cilia- and Flagella-Associated Protein (CFAP) family

Primary Research Area

Cilia biology, ciliopathies, flagellar assembly

Background: CFAP92 and Its Role in Cilia Biology

CFAP92 (Cilia- and Flagella-Associated Protein 92) is a member of the conserved family of proteins essential for the structural integrity and motility of cilia and flagella. Cilia are hair-like organelles present on the surface of many eukaryotic cell types; they play critical roles in cell signaling, fluid movement, and sensory perception. Dysfunction of ciliary proteins is associated with a spectrum of inherited disorders collectively termed ciliopathies, which can affect multiple organ systems including the respiratory tract, reproductive organs, kidneys, and the central nervous system.

CFAP92 is thought to be involved in the assembly and maintenance of the axonemal structure within motile cilia. The axoneme, composed of microtubule doublets and associated dynein motor complexes, is the core structural unit driving ciliary beat. Research into CFAP92 and related CFAP family members contributes to our understanding of primary ciliary dyskinesia (PCD), male infertility, and other ciliopathy phenotypes.

Product Specifications and Storage Guidelines

Recombinant Human CFAP92 Protein (SKU: rp376081) is produced recombinantly and supplied as a research-grade reagent. The following general guidelines apply to recombinant proteins of this class; researchers should always consult the product datasheet supplied with each lot for definitive specifications including purity, formulation, and concentration.

• Expression System: Confirm with the product datasheet; common systems for recombinant CFAP-family proteins include E. coli, insect cell (baculovirus), or mammalian cell expression.
• Purity: Typically >85% as determined by SDS-PAGE; refer to the Certificate of Analysis (CoA) for lot-specific data.
• Formulation: Generally supplied in a buffered solution; specific buffer composition is provided on the product datasheet.
• Storage: Store at -80°C for long-term storage. Avoid repeated freeze-thaw cycles, as these can reduce protein activity and integrity. Aliquot upon receipt if multiple uses are anticipated.
• Stability: When stored as directed, recombinant proteins are typically stable for 12 months from the date of receipt. Refer to the CoA for confirmed stability data.

Recommended Handling and Reconstitution Protocol

Proper handling of recombinant proteins is essential to maintain biological activity and ensure reproducible experimental results. Follow the steps below when working with Recombinant Human CFAP92 Protein:

1. Thawing: Thaw the protein gently on ice or at 4°C. Do not thaw at room temperature or above, as this may accelerate degradation.
2. Reconstitution (if lyophilized): If the product is supplied in lyophilized form, reconstitute with the volume of sterile deionized water or buffer specified on the datasheet. Allow the lyophilized cake to dissolve completely by gently swirling — do not vortex vigorously.
3. Centrifugation: Briefly centrifuge the vial (e.g., 5–10 seconds at low speed) before opening to collect any material from the lid or walls.
4. Aliquoting: Prepare single-use aliquots at appropriate working concentrations to prevent repeated freeze-thaw cycles.
5. Carrier Protein: For dilute working solutions, addition of a carrier protein (e.g., 0.1% BSA) may help maintain stability and minimize adsorption to tube surfaces.
6. Working Conditions: Keep the protein on ice during experiments. Minimize the time the protein is at room temperature.

Note: Always refer to the lot-specific Certificate of Analysis and product datasheet for reconstitution volumes, working concentrations, and validated assay conditions.

Potential Research Applications

Recombinant Human CFAP92 Protein is intended for research use only and may be applicable in the following experimental contexts:

• Protein–Protein Interaction Studies: Use as bait or prey in pull-down assays or co-immunoprecipitation experiments to identify binding partners within the ciliary proteome.
• Antibody Production and Validation: Serve as an immunogen for generating anti-CFAP92 antibodies, or as a positive control for validating existing antibody reagents by Western blot or ELISA.
• Structural Biology: Support crystallography, cryo-electron microscopy, or other structural studies aimed at elucidating the three-dimensional architecture of CFAP92 and its complexes.
• Biochemical Assays: Investigate the biochemical properties of CFAP92, including binding affinities, post-translational modification sites, and interactions with axonemal components.
• Ciliopathy Disease Modeling: Complement cell-based and in vivo models of ciliary dysfunction by providing a characterized recombinant reference protein for mechanistic studies.

This product is for research use only (RUO) and is not intended for diagnostic, therapeutic, or clinical use.

Quality Control and Regulatory Considerations

Aladdin Scientific is committed to supplying research reagents that meet rigorous quality standards. For Recombinant Human CFAP92 Protein (rp376081), the following quality control principles apply:

• Lot-Specific Certificate of Analysis (CoA): Each lot is accompanied by a CoA documenting purity, identity, and functional test results where applicable. Researchers should download or request the CoA for their specific lot number.
• SDS-PAGE Verification: Protein purity and apparent molecular weight are routinely verified by SDS-PAGE under reducing conditions.
• Endotoxin Testing: For applications sensitive to endotoxin contamination (e.g., cell-based assays), verify endotoxin levels from the CoA or contact Aladdin Scientific technical support for lot-specific data.
• Research Use Only (RUO): This product has not been evaluated by regulatory agencies for safety or efficacy in medical applications. It must not be used for clinical, diagnostic, or therapeutic purposes.
• Biosafety: Handle all recombinant proteins following your institution's biosafety guidelines. Consult the Safety Data Sheet (SDS) before use.

Frequently asked questions

What is CFAP92 and why is it important for research?

CFAP92 (Cilia- and Flagella-Associated Protein 92) is a protein involved in the structure and function of cilia and flagella — organelles critical for cell motility, fluid flow, and signaling. Research into CFAP92 is important for understanding ciliopathies, a group of genetic disorders caused by defective cilia, including primary ciliary dyskinesia and conditions affecting fertility, respiratory health, and organ development.

How should I store Recombinant Human CFAP92 Protein to maintain activity?

Store Recombinant Human CFAP92 Protein at -80°C for long-term preservation. Upon receipt, aliquot the protein into single-use volumes to avoid repeated freeze-thaw cycles, which can degrade protein integrity and reduce activity. Always thaw aliquots gently on ice rather than at room temperature.

What are the recommended applications for Recombinant Human CFAP92 Protein?

This protein is suitable for research applications including protein–protein interaction studies, antibody generation and validation, structural biology investigations, and biochemical characterization of ciliary components. It may also serve as a reference standard in assays related to ciliopathy research. This product is for research use only and is not intended for clinical or diagnostic applications.

Where can I obtain the Certificate of Analysis (CoA) for my lot of rp376081?

The Certificate of Analysis for each lot of Recombinant Human CFAP92 Protein (rp376081) can be downloaded from the Aladdin Scientific product page by entering your lot number, or by contacting Aladdin Scientific customer support directly. The CoA contains lot-specific data on purity, molecular weight, and quality testing results.

Can this product be used in cell-based assays, and what endotoxin considerations apply?

Recombinant Human CFAP92 Protein may be used in cell-based assays; however, researchers must verify endotoxin levels from the lot-specific CoA before use, as endotoxin contamination can confound cellular responses. If endotoxin levels are a critical concern for your assay, contact Aladdin Scientific technical support to obtain lot-specific endotoxin data or to inquire about low-endotoxin options.

References

1. Reiter JF, Leroux MR. Genes and molecular pathways underpinning ciliopathies. Nature Reviews Molecular Cell Biology. 2017;18(9):533-547. doi:10.1038/nrm.2017.60
2. Mitchison HM, Valente EM. Motile and non-motile cilia in human pathology: from function to phenotypes. Journal of Pathology. 2017;241(2):294-309. doi:10.1002/path.4843
3. Pazour GJ, Agrin N, Leszyk J, Witman GB. Proteomic analysis of a eukaryotic cilium. Journal of Cell Biology. 2005;170(1):103-113. doi:10.1083/jcb.200504008

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.