Recombinant Human FSBP Protein: Properties & Research Use

Recombinant Human FSBP Protein is a recombinantly produced form of the human fibronectin synergy-binding protein, offered by Aladdin Scientific (SKU: rp376837) as a high-quality reagent for transcription factor research and related biochemical studies.

Product Name

Recombinant Human FSBP Protein

SKU

rp376837

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant protein; transcription factor family

Supplier

Aladdin Scientific

Reference Number

absea#PP-10363

What Is FSBP? Gene and Protein Background

FSBP (Fibronectin Synergy-Binding Protein) is a human nuclear protein that belongs to the transcription factor superfamily. The FSBP gene encodes a protein involved in the regulation of gene expression, with reported roles in binding to specific DNA regulatory elements. As a transcription factor, FSBP is implicated in modulating downstream gene networks relevant to cell biology and potentially disease pathways.

Recombinant Human FSBP Protein is produced using recombinant DNA technology, enabling researchers to obtain a well-defined, reproducible protein for in vitro biochemical, binding, and functional assays. The recombinant format ensures batch-to-batch consistency, which is critical for quantitative research applications.

Recombinant Protein Production and Formulation

Recombinant Human FSBP Protein (SKU: rp376837) is manufactured through standard recombinant expression systems. Recombinant proteins are typically expressed in suitable host systems such as E. coli, insect cells (baculovirus), mammalian cells, or yeast, depending on the folding and post-translational modification requirements of the target protein. After expression, the protein is purified using affinity chromatography and other downstream purification steps to achieve the required purity suitable for research use.

Researchers should consult the product datasheet for specific details regarding the expression host, tag information, purity grade, recommended storage conditions, and reconstitution guidelines. Proper handling, including storage at recommended temperatures and avoidance of repeated freeze-thaw cycles, is essential to preserve protein activity and integrity.

Research Applications of Recombinant Human FSBP Protein

Recombinant Human FSBP Protein is suitable for a range of in vitro research applications typically associated with transcription factor proteins, including:

• Protein–DNA binding assays: Electrophoretic mobility shift assays (EMSA) and similar methods to characterize FSBP DNA-binding activity.
• Protein–protein interaction studies: Pull-down assays and co-immunoprecipitation to identify FSBP binding partners.
• Antibody validation: Use as a positive control antigen for anti-FSBP antibody testing by Western blot, ELISA, or dot blot.
• Functional and enzymatic assays: Investigation of FSBP transcriptional regulatory function in reconstituted in vitro transcription systems.
• Structural studies: Biophysical characterization such as circular dichroism (CD) or size-exclusion chromatography (SEC) to assess protein folding and oligomeric state.

These applications support a broad range of basic research goals in transcription biology, gene regulation, and related disease mechanism studies.

Storage, Handling, and Quality Considerations

To maintain the activity and stability of Recombinant Human FSBP Protein, researchers should adhere to the following general best practices:

• Store the protein according to the conditions specified in the product datasheet, typically at −20°C or −80°C for long-term storage.
• Avoid repeated freeze-thaw cycles by preparing single-use aliquots upon first thaw.
• Reconstitute lyophilized protein in the recommended buffer and allow the solution to equilibrate gently; do not vortex vigorously.
• Verify protein integrity and concentration before use, using methods such as SDS-PAGE and Bradford or BCA assays.
• Handle with standard laboratory safety precautions appropriate for research-grade recombinant proteins.

For specific purity grade, endotoxin levels, biological activity data, and lot-specific quality control results, please refer to the Certificate of Analysis (CoA) provided with each shipment or available upon request from Aladdin Scientific.

Frequently asked questions

What is Recombinant Human FSBP Protein used for in research?

Recombinant Human FSBP Protein is used in a variety of in vitro research applications, including protein–DNA binding assays (e.g., EMSA), protein–protein interaction studies, antibody validation as a positive control antigen, and structural or biophysical characterization of the FSBP transcription factor.

How should Recombinant Human FSBP Protein (rp376837) be stored?

The protein should be stored according to the conditions specified in the product datasheet, typically at −20°C or −80°C for long-term stability. To preserve activity, prepare single-use aliquots to avoid repeated freeze-thaw cycles and refer to the Certificate of Analysis for lot-specific recommendations.

What expression system is used to produce Recombinant Human FSBP Protein?

The specific expression host for rp376837 is detailed in the product datasheet and Certificate of Analysis. Common expression systems for recombinant proteins include E. coli, insect cells, mammalian cells, and yeast; please consult the product documentation or contact Aladdin Scientific for confirmed details.

Can Recombinant Human FSBP Protein be used as a positive control in Western blot experiments?

Yes, recombinant proteins such as FSBP are commonly used as positive control antigens in Western blot, ELISA, and dot blot assays to validate antibody specificity and sensitivity. Confirm suitability with the product datasheet before use.

References

1. Aladdin Scientific. Recombinant Human FSBP Protein, SKU rp376837. Product datasheet reference: absea#PP-10363.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.