Recombinant Human GABPB1 Protein: Properties & Applications

Recombinant Human GABPB1 Protein is a key regulatory subunit of the GA-binding protein (GABP) transcription factor complex, playing essential roles in the activation of genes containing Ets-binding motifs across a wide range of cellular processes.

Protein Name

Recombinant Human GABPB1 Protein

SKU

rp367874

Protein Family

ETS family transcription factor subunit (GABP complex beta subunit)

Species Origin

Human (Homo sapiens)

Primary Function

Transcriptional co-activation via interaction with GABPA; regulates genes with Ets-binding motifs

Research Applications

Transcription factor studies, protein–protein interaction assays, gene regulation research, EMSA

What Is GABPB1? Biological Background

GA-binding protein subunit beta-1 (GABPB1) is a member of the ETS-domain transcription factor superfamily. Unlike many ETS proteins that function as monomers, GABPB1 operates as part of a heterotetrameric complex together with GABPA (also known as NRF-2 alpha). GABPA provides the DNA-binding ETS domain that recognizes the consensus sequence 5'-CTTCC-3', while GABPB1 contributes ankyrin repeat domains that mediate protein–protein interactions and nuclear localization, as well as a transcriptional activation domain that drives target gene expression.

The GABP complex is constitutively expressed in most human tissues and regulates a broad spectrum of genes involved in mitochondrial biogenesis, cell cycle progression, immune cell differentiation, and oxidative phosphorylation. Because of its fundamental role in coordinating nuclear-encoded mitochondrial gene expression, GABPB1 is of significant interest in research areas including metabolic disease, oncology, and immunology.

Molecular Features of Recombinant Human GABPB1 Protein

Recombinant Human GABPB1 Protein (SKU: rp367874) is produced to support in vitro biochemical and cell-free studies where endogenous protein quantity or modification state cannot be precisely controlled. As a recombinant reagent, it enables researchers to perform reproducible, scalable experiments without relying on immunoprecipitation from cell lysates.

Key molecular features of GABPB1 include its ankyrin repeat domain, which is responsible for binding to the pointed (PNT) domain of GABPA and facilitating formation of the functional heterocomplex. The protein also contains a leucine zipper-like motif that supports dimerization of GABPB1 subunits, enabling the assembly of the full GABPA2/GABPB1-2 heterotetramer on DNA. Researchers should confirm specific tag configuration, expression host, purity grade, and formulation details directly on the product datasheet for rp367874 prior to experimental design.

Research Applications

Recombinant Human GABPB1 Protein is suited for a variety of in vitro research applications:

• Electrophoretic Mobility Shift Assay (EMSA): GABPB1, in combination with GABPA, can be used to validate Ets-motif binding activity and study protein–DNA complex formation under defined conditions.
• Protein–Protein Interaction Studies: The ankyrin repeat domain of GABPB1 makes it a valuable tool for pull-down assays, co-immunoprecipitation controls, and surface plasmon resonance (SPR) experiments investigating GABPA–GABPB1 complex assembly.
• Transcription Factor Complex Reconstitution: Cell-free reconstitution of the GABP heterotetramer enables mechanistic dissection of co-activator recruitment and promoter selectivity.
• Antibody Validation and Immunoassay Development: Recombinant GABPB1 serves as a well-defined antigen standard for validating anti-GABPB1 antibodies, optimizing ELISA formats, and establishing Western blot controls.
• Drug Discovery and Screening: As a transcription factor subunit implicated in oncogenesis and mitochondrial regulation, GABPB1 is a candidate target for small-molecule interaction screens.

Handling, Storage, and Quality Considerations

Proper handling of recombinant proteins is critical for maintaining activity and reproducibility. General best-practice recommendations for recombinant transcription factor subunits include:

• Storage: Store at -80 °C upon receipt for long-term stability. Avoid repeated freeze–thaw cycles, which can cause aggregation and loss of biological activity. Aliquot into single-use volumes before freezing where possible.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer recommended on the product datasheet. Allow the vial to equilibrate to room temperature before opening.
• Working Conditions: Conduct all manipulations on ice or at 4 °C to minimize proteolytic degradation. Use low-protein-binding tubes to reduce surface adsorption losses.
• Quality Controls: Verify protein integrity by SDS-PAGE or analytical SEC prior to critical experiments. Confirm functional activity using an appropriate binding or activity assay as described in the product datasheet.

For exact storage temperature, buffer composition, concentration, purity percentage, and shelf life of rp367874, always refer to the current product datasheet available on the Aladdin Scientific website.

Why Choose Aladdin Scientific for Recombinant GABPB1?

Aladdin Scientific is committed to supplying research-grade recombinant proteins that meet the reproducibility demands of modern biochemical research. Recombinant Human GABPB1 Protein (rp367874) is part of Aladdin's catalog of recombinant transcription factors and regulatory proteins, offered to support investigators working on gene expression regulation, transcription factor biology, and related disease mechanisms.

When sourcing recombinant proteins, researchers and lab managers should evaluate: expression system and host organism, tag type and position, purity determination method, functional validation data, batch-to-batch consistency documentation, and technical support availability. Aladdin Scientific provides product datasheets and technical support to assist with experimental design. For specific lot-release data, certificate of analysis (CoA) requests, or custom formulation inquiries regarding rp367874, contact the Aladdin Scientific technical team directly.

Frequently asked questions

What is the function of GABPB1 in the GABP transcription factor complex?

GABPB1 serves as the beta subunit of the GA-binding protein (GABP) complex. It contains ankyrin repeat domains that interact with the GABPA subunit, facilitates nuclear localization of the complex, and provides a transcriptional activation domain. Together with GABPA, it forms a heterotetramer that binds Ets-motif-containing promoters and drives transcription of target genes involved in mitochondrial biogenesis, cell cycle control, and immune function.

What applications is Recombinant Human GABPB1 Protein (rp367874) suitable for?

Recombinant Human GABPB1 Protein is suitable for EMSA to study protein–DNA interactions, pull-down and co-immunoprecipitation assays for protein–protein interaction studies, in vitro transcription factor complex reconstitution, antibody validation as an antigen standard, Western blot controls, ELISA development, and small-molecule interaction screening. Confirm suitability for each specific application with the product datasheet.

How should Recombinant Human GABPB1 Protein be stored to maintain activity?

For long-term storage, keep the protein at -80 °C and avoid repeated freeze–thaw cycles by preparing single-use aliquots. Conduct manipulations on ice or at 4 °C to minimize degradation. Always consult the specific product datasheet for rp367874 for the exact recommended storage conditions, buffer composition, and shelf life.

What distinguishes GABPB1 from other ETS family transcription factor subunits?

Unlike most ETS family proteins that contain a DNA-binding ETS domain themselves, GABPB1 lacks a direct ETS DNA-binding domain. Instead, it functions as an obligate partner for GABPA, contributing ankyrin repeats for protein–protein interaction, a leucine zipper-like motif for dimerization, and a transactivation domain. This unique architecture means GABPB1 regulates transcription indirectly through complex formation rather than direct DNA contact.

References

1. Chinenov Y, Kerppola TK. Close encounters of many kinds: Fos-Jun interactions that mediate transcription regulatory specificity. Oncogene. 2001;20(19):2438-2452. [Cited as background on ETS-family heterocomplex transcription factor biology — verify applicability to GABPB1 specifically]
2. Rosmarin AG, Yang Z, Resendes KK. Transcriptional regulation in myeloid cells: the roles of the myeloid zinc finger gene (MZF-1) and growth factor independence-1 (Gfi-1) in differentiation and development. Semin Immunol. 2005. [Background reference on ETS factors in immune regulation — confirm GABPB1-specific relevance]
3. Aladdin Scientific. Recombinant Human GABPB1 Protein (rp367874) Product Datasheet. Aladdin Scientific; [date not specified in source material].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.