Recombinant Human GALNT5 Protein: Key FAQs & Specs

Recombinant Human GALNT5 Protein is a research-grade recombinant enzyme representing the human polypeptide N-acetylgalactosaminyltransferase 5, essential for initiating mucin-type O-glycosylation and widely used in glycobiology studies.

Product Name

Recombinant Human GALNT5 Protein

SKU

rp365332

Protein Family

Polypeptide GalNAc-transferase (GALNT) family

Biological Function

Catalyzes transfer of N-acetylgalactosamine to serine/threonine residues, initiating mucin-type O-glycosylation

Gene Name

GALNT5

Species

Human (Homo sapiens)

Application

Glycobiology research, enzyme activity assays, substrate specificity studies

What Is Recombinant Human GALNT5 Protein?

Recombinant Human GALNT5 Protein (SKU: rp365332) is a recombinant form of the human enzyme polypeptide N-acetylgalactosaminyltransferase 5, encoded by the GALNT5 gene. This enzyme belongs to the large family of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GALNTs), which are responsible for the initial step of mucin-type O-linked glycosylation — a post-translational modification critical for cell signaling, immune recognition, and protein stability.

GALNT5 transfers N-acetylgalactosamine (GalNAc) from the sugar nucleotide donor UDP-GalNAc onto serine and threonine residues of target polypeptides. The resulting O-GalNAc modification serves as the core structure upon which more complex O-glycan chains are elaborated by subsequent glycosyltransferases. GALNT5 displays a distinct substrate specificity compared to other family members, making it a valuable tool for investigating site-specific O-glycosylation.

Specifications, Purity & Quality Control

Aladdin Scientific's Recombinant Human GALNT5 Protein is produced to research-reagent standards. Key quality attributes that scientists should confirm at the time of ordering or upon receipt include:

• Expression System: Typically expressed in mammalian or insect cell systems to preserve post-translational modifications relevant to enzymatic activity. Confirm the specific host system with the product datasheet (reference: absea#PP-894).
• Purity: Research-grade recombinant proteins are generally supplied at ≥85–95% purity as assessed by SDS-PAGE and/or HPLC; verify the exact specification on the current Certificate of Analysis (CoA).
• Formulation: Typically supplied in a physiologically compatible buffer. Exact buffer composition, protein concentration, and any carrier protein or stabilizer content should be confirmed on the CoA.
• Endotoxin Level: For cell-based assays, low endotoxin levels are critical. Check the product CoA for the tested endotoxin value.
• Activity: Enzymatic activity may be confirmed by in vitro glycosyltransferase assay; consult the datasheet for validated activity data.

Always request and review the lot-specific CoA before use to confirm all critical quality parameters for your application.

Recommended Applications & Experimental Use

Recombinant Human GALNT5 Protein is suited for a range of in vitro research applications in glycobiology and biochemistry:

• Enzyme Activity Assays: Use as the enzyme source in in vitro glycosyltransferase assays to study GalNAc transfer kinetics and substrate preferences.
• Substrate Specificity Profiling: Investigate which peptide or protein substrates are preferentially modified by GALNT5, aiding in the mapping of O-glycosylation sites.
• Biochemical Studies: Explore GALNT5 protein–protein interactions and its role in the Golgi glycosylation machinery.
• Disease Research: GALNT members including GALNT5 have been associated with altered glycosylation patterns in cancer and other diseases; this recombinant protein can serve as a tool in mechanistic studies.
• Positive Control: Use as a positive control or standard in immunoassays or Western blotting experiments targeting GALNT5.

For best results, optimize reaction conditions (pH, temperature, buffer composition, UDP-GalNAc concentration) according to published protocols for GALNT family enzymes.

Storage, Handling & Stability

Proper storage and handling are essential to maintain the activity and integrity of Recombinant Human GALNT5 Protein:

• Storage Temperature: Store at –20°C or –80°C as specified on the product datasheet. Avoid repeated freeze–thaw cycles, which can reduce enzymatic activity and cause aggregation.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimize freeze–thaw cycles.
• Working Solutions: Keep on ice during experimental setup. Do not leave at room temperature for extended periods.
• Carrier Protein: If the formulation does not contain a stabilizing carrier protein (e.g., BSA), consider adding one for long-term stability at low concentrations; verify with the product CoA.
• Shelf Life: Consult the product datasheet or CoA for confirmed shelf life. Typical recombinant proteins stored correctly are stable for 12 months or more; exact data should be confirmed from the supplier.

If any visible precipitation or turbidity is observed upon thawing, gently centrifuge and use the clarified supernatant. Contact Aladdin Scientific technical support if product quality concerns arise.

Ordering, Availability & Technical Support

Recombinant Human GALNT5 Protein is available from Aladdin Scientific under SKU rp365332. Researchers and procurement teams can place orders through the Aladdin Scientific website or by contacting the sales team directly.

• Product Reference: absea#PP-894
• Available Sizes: Contact Aladdin Scientific for available quantities and bulk pricing options.
• Custom Requirements: For custom formulations, concentrations, or conjugation services, contact technical support to discuss project-specific needs.
• Documentation: Safety Data Sheets (SDS), Certificates of Analysis (CoA), and product datasheets are available upon request or through the product page.
• Technical Support: Aladdin Scientific's scientific team can assist with protocol optimization, troubleshooting, and application guidance for GALNT5 protein research.

For the most current pricing, stock availability, and shipping options, please visit the Aladdin Scientific website or contact your regional representative.

Frequently asked questions

What is the biological function of GALNT5?

GALNT5 (polypeptide N-acetylgalactosaminyltransferase 5) catalyzes the first committed step of mucin-type O-linked glycosylation by transferring GalNAc from UDP-GalNAc to serine and threonine residues on target proteins. This modification plays roles in protein folding, stability, cell adhesion, and intercellular signaling. Each GALNT family member exhibits distinct substrate specificity, and GALNT5 has been studied in the context of tissue-specific glycosylation patterns and cancer biology.

What expression system is used to produce the Recombinant Human GALNT5 Protein (rp365332)?

The specific expression system used to produce SKU rp365332 should be confirmed on the product datasheet (reference: absea#PP-894) or the lot-specific Certificate of Analysis. Recombinant GALNT family proteins are commonly expressed in mammalian or insect (baculovirus) cell systems to ensure proper folding and relevant post-translational modifications necessary for enzymatic activity. Please contact Aladdin Scientific or refer to the product documentation for confirmed details.

How should Recombinant Human GALNT5 Protein be stored to maintain activity?

Recombinant Human GALNT5 Protein should be stored at –20°C or –80°C as specified in the product datasheet. To preserve enzymatic activity, avoid repeated freeze–thaw cycles by preparing single-use aliquots upon first thaw. Keep the protein on ice during experimental workflows and do not leave it at room temperature for prolonged periods. Always verify storage conditions on the lot-specific CoA.

What applications is Recombinant Human GALNT5 Protein validated for?

Recombinant Human GALNT5 Protein is suitable for in vitro glycosyltransferase enzyme activity assays, substrate specificity profiling, biochemical interaction studies, and as a positive control in immunoassays or Western blotting targeting GALNT5. It is also applicable in disease-mechanism studies where aberrant O-glycosylation is implicated. Validated applications and specific activity data should be confirmed in the current product datasheet (absea#PP-894).

Is Recombinant Human GALNT5 Protein (rp365332) suitable for cell-based assays?

Suitability for cell-based assays depends on the endotoxin level, formulation, and purity of the specific lot. Researchers should review the Certificate of Analysis for endotoxin content (typically expressed as EU/mg) and confirm the formulation is compatible with cell culture conditions. Contact Aladdin Scientific technical support for guidance on cell-based application compatibility for SKU rp365332.

How can I order Recombinant Human GALNT5 Protein and what documentation is available?

Recombinant Human GALNT5 Protein (rp365332) can be ordered via the Aladdin Scientific website or through your regional sales representative. Available documentation includes the product datasheet (absea#PP-894), Safety Data Sheet (SDS), and lot-specific Certificate of Analysis (CoA). For bulk quantities, custom formulations, or additional technical documentation, contact Aladdin Scientific directly.

References

1. Product datasheet: Recombinant Human GALNT5 Protein, Aladdin Scientific, SKU rp365332, reference absea#PP-894.
2. Bennett EP, Mandel U, Clausen H, Gerken TA, Fritz TA, Tabak LA. Control of mucin-type O-glycosylation: a classification of the polypeptide GalNAc-transferase gene family. Glycobiology. 2012;22(6):736-756. doi:10.1093/glycob/cwr182
3. Schjoldager KT, Clausen H. Site-specific protein O-glycosylation modulates proprotein processing - deciphering specific functions of the large polypeptide GalNAc-transferase gene family. Biochimica et Biophysica Acta. 2012;1820(12):2079-2094. doi:10.1016/j.bbagen.2012.09.014

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.