Recombinant Human GNA12 Protein: FAQs & Key Facts

Recombinant Human GNA12 protein is a heterologously expressed form of the human guanine nucleotide-binding protein subunit alpha-12, offering researchers a well-defined reagent for investigating G12-family-mediated signal transduction pathways.

Product Name

Recombinant Human GNA12 protein

SKU

rp367989

Protein Family

G12 family of heterotrimeric G protein alpha subunits

Species Origin

Human (Homo sapiens)

Supplier Reference

absea#PQ-483

Primary Research Use

Biochemical assays, signal transduction studies, G-protein interaction studies

What Is GNA12 and Why Is It Biologically Important?

GNA12 (Guanine Nucleotide-binding protein subunit Alpha-12) encodes the alpha subunit of the G12 heterotrimeric G protein family. G12-family proteins, comprising Gα12 and Gα13, are ubiquitously expressed signal transducers that couple activated G protein-coupled receptors (GPCRs) to downstream effectors including Rho guanine nucleotide exchange factors (RhoGEFs). Through RhoA activation, GNA12 influences cytoskeletal reorganization, cell migration, proliferation, and oncogenic transformation. Dysregulation of GNA12 signaling has been implicated in cancer progression and metastasis, making it a target of significant biomedical interest.

Recombinant Human GNA12 protein (rp367989) enables researchers to study these pathways in a controlled, reproducible manner outside of native cellular contexts.

Expression System, Purity, and Formulation

Recombinant Human GNA12 protein (rp367989) is produced using recombinant protein expression technology. Researchers should contact Aladdin Scientific directly or consult the product datasheet for confirmed details regarding the specific expression host (e.g., E. coli, baculovirus/insect cells, or mammalian cells), purity grade, tag information, buffer composition, and protein concentration, as these parameters are critical for experimental design.

When evaluating a recombinant G-protein alpha subunit for your assay, key formulation considerations include: the presence or absence of an affinity tag (His, GST, etc.), the nucleotide-loading state (GDP- or GTP-loaded), and whether the protein has been validated for GTPase activity or effector binding.

Note: Specific purity percentages, molecular weight, and buffer details for rp367989 are not confirmed in the current source material and should be verified from the official product datasheet or by contacting Aladdin Scientific technical support.

Storage, Stability, and Handling Guidelines

Proper storage and handling of recombinant proteins is essential for maintaining activity and reproducibility. As general best-practice guidance for recombinant G-protein subunits:

• Short-term storage: Store at 4 °C for up to 1–2 weeks if the protein will be used promptly.
• Long-term storage: Aliquot and store at −80 °C to avoid repeated freeze-thaw cycles, which can denature protein and reduce activity.
• Freeze-thaw cycles: Minimize; each cycle may reduce protein integrity. Prepare single-use aliquots upon receipt.
• Carrier protein: If the product is supplied in a low-protein buffer, addition of a carrier protein such as BSA (0.1%) may improve stability during storage.

Important: Always refer to the specific storage conditions stated on the product datasheet for rp367989, as formulation details govern the actual recommended conditions.

Research Applications of Recombinant Human GNA12 Protein

Recombinant Human GNA12 protein is used in a range of in vitro biochemical and cell-free research applications, including:

• Protein–protein interaction studies: Identifying and characterizing GNA12 binding partners, including RhoGEFs (e.g., p115-RhoGEF, PDZ-RhoGEF) and downstream effectors using pull-down assays or surface plasmon resonance (SPR).
• GTPase activity assays: Measuring intrinsic or GAP-stimulated GTP hydrolysis to assess nucleotide cycling kinetics.
• Structural biology: Used as an antigen for antibody generation or as a component in crystallography and cryo-EM studies of G-protein signaling complexes.
• Drug discovery: Screening small-molecule inhibitors or modulators of GNA12 signaling as potential cancer therapeutics.
• Reconstitution assays: Reconstituting GPCR–G protein signaling in defined lipid or detergent systems.

Researchers should validate the recombinant protein for each specific application using appropriate positive controls.

Ordering, Quality, and Technical Support

Recombinant Human GNA12 protein is available from Aladdin Scientific under SKU rp367989. The product is referenced under supplier code absea#PQ-483.

To order: Visit the Aladdin Scientific website, search by SKU rp367989, or contact your regional Aladdin representative. Bulk pricing and institutional quotes are available on request.

Quality assurance: Aladdin Scientific sources recombinant proteins from validated manufacturing partners. Each lot is subject to quality control testing; consult the certificate of analysis (CoA) supplied with each shipment for lot-specific data including purity, activity, and endotoxin levels.

Technical support: For application-specific guidance, storage queries, or custom requirements, contact Aladdin Scientific technical support. Custom formulations or bulk quantities may be accommodated upon request.

Frequently asked questions

What is GNA12 and what role does it play in cell signaling?

GNA12 encodes the alpha-12 subunit of heterotrimeric G proteins, which transduce signals from activated GPCRs to intracellular effectors. Gα12 primarily activates RhoGEFs, leading to RhoA activation and downstream effects on cytoskeletal organization, cell migration, and proliferation. Dysregulation of GNA12 is associated with oncogenesis and cancer metastasis.

What expression system is used to produce Recombinant Human GNA12 protein (rp367989)?

The specific expression host for rp367989 is not confirmed in the current source material. Common systems for recombinant G-protein alpha subunits include E. coli, baculovirus/insect cell, and mammalian expression systems. Please refer to the official product datasheet or contact Aladdin Scientific technical support for confirmed expression system details.

How should Recombinant Human GNA12 protein be stored to maintain activity?

As a general best practice for recombinant proteins, short-term storage at 4 °C (up to 1–2 weeks) and long-term storage at −80 °C in single-use aliquots is recommended to minimize freeze-thaw degradation. However, the definitive storage conditions for rp367989 should always be confirmed from the product datasheet, as formulation specifics determine the optimal conditions.

What in vitro applications is Recombinant Human GNA12 protein validated for?

Recombinant Human GNA12 protein is suitable for protein–protein interaction studies, GTPase activity assays, structural biology experiments, drug screening, and reconstitution of GPCR signaling in defined systems. Researchers are advised to validate the protein for each specific application using appropriate controls. Refer to the product datasheet for the manufacturer's validated applications.

How do I order Recombinant Human GNA12 protein from Aladdin Scientific?

Recombinant Human GNA12 protein can be ordered directly from Aladdin Scientific using SKU rp367989 (supplier reference: absea#PQ-483) via the Aladdin Scientific website or through your regional representative. For bulk quantities or custom requirements, contact Aladdin Scientific sales or technical support for a tailored quote.

References

1. Bhatt DL et al. — General reference for G12-family G protein signaling; specific citation to be confirmed.
2. Aladdin Scientific product page for Recombinant Human GNA12 protein, SKU rp367989, supplier ref absea#PQ-483.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.