Recombinant Human GNPDA2 Protein: FAQs & Key Facts

Recombinant Human GNPDA2 Protein is a research-grade recombinant enzyme representing the human glucosamine-6-phosphate deaminase 2 isoform, produced for use in metabolic pathway studies, protein interaction assays, and obesity-related research applications.

Product Name

Recombinant Human GNPDA2 Protein

SKU

rp371064

Gene Name

GNPDA2 (Glucosamine-6-Phosphate Deaminase 2)

Species Origin

Human (Homo sapiens)

Protein Function

Catalyzes the deamination of glucosamine-6-phosphate to fructose-6-phosphate in the hexosamine biosynthetic pathway

Research Relevance

GNPDA2 locus is associated with body mass index (BMI) and obesity susceptibility in genome-wide association studies

Supplier

Aladdin Scientific

What Is GNPDA2 and Why Is It Scientifically Relevant?

GNPDA2 (Glucosamine-6-Phosphate Deaminase 2) is an enzyme that catalyzes the reversible isomerization and deamination of glucosamine-6-phosphate (GlcN-6-P) to fructose-6-phosphate and ammonium ion. This reaction is a key step in the hexosamine biosynthetic pathway, which plays important roles in nutrient sensing, glycoprotein biosynthesis, and cellular metabolism.

The GNPDA2 gene gained significant attention in metabolic research following genome-wide association studies (GWAS) that identified variants near the GNPDA2 locus as significantly associated with body mass index (BMI) and obesity risk in large human cohorts. This association has made GNPDA2 a subject of investigation in studies of energy homeostasis, adipogenesis, and hypothalamic signaling pathways related to appetite regulation.

Recombinant Human GNPDA2 Protein enables researchers to study the enzymatic properties, protein-protein interactions, and downstream signaling functions of this protein in vitro, providing a controlled and reproducible tool for mechanistic investigations.

Frequently Asked Questions About Product Specifications

What expression system is used to produce Recombinant Human GNPDA2 Protein?
The specific expression system used for rp371064 should be confirmed against the official product datasheet. Common systems for recombinant human proteins include E. coli, baculovirus-infected insect cells (Sf9), and mammalian expression systems (HEK293). Please refer to the product datasheet or contact Aladdin Scientific technical support for confirmed details.

What is the expected purity of the protein?
Research-grade recombinant proteins are typically supplied at >85–95% purity as assessed by SDS-PAGE. The precise purity specification for rp371064 should be verified on the current product datasheet available on the Aladdin Scientific website.

Does the protein include a purification tag?
Many recombinant proteins are produced with affinity tags (e.g., His-tag, GST-tag) to facilitate purification. Whether a tag is present or removed in the final product should be confirmed on the official product datasheet for rp371064.

What is the formulation and concentration of the supplied protein?
Formulation details such as buffer composition, protein concentration, and the presence of carrier proteins or stabilizers are product-specific and must be verified on the current datasheet. Contact Aladdin Scientific for the most up-to-date specifications.

Storage, Handling, and Stability Guidelines

Proper storage and handling of recombinant proteins is critical for maintaining biological activity and experimental reproducibility. While product-specific conditions must always be confirmed on the datasheet, the following general best practices apply to recombinant human proteins such as GNPDA2:

• Short-term storage: Store at 4°C for up to one week if the protein will be used promptly after receipt.
• Long-term storage: Aliquot the protein into single-use volumes and store at −20°C or −80°C to avoid repeated freeze-thaw cycles, which can lead to protein degradation and loss of activity.
• Freeze-thaw cycles: Minimize the number of freeze-thaw cycles. It is strongly recommended to prepare single-use aliquots upon first thaw.
• Carrier protein: If the protein is supplied without a carrier such as BSA, consider adding a carrier protein to low-concentration aliquots to prevent adsorption to tube walls.
• Sterility: Handle under aseptic conditions if the protein is intended for cell-based assays.

Always consult the official Aladdin Scientific product datasheet for rp371064 for confirmed storage temperature, shelf life, and reconstitution instructions if applicable.

Recommended Research Applications

Recombinant Human GNPDA2 Protein is suited for a range of in vitro research applications. Researchers should validate the protein's performance in their specific assay format before use in critical experiments. Typical applications for recombinant proteins of this type include:

• Enzyme activity assays: Measuring the deamination of glucosamine-6-phosphate to characterize kinetic parameters (K_m, V_max) or test the effect of inhibitors and modulators on GNPDA2 enzymatic function.
• Western blotting (positive control): Use as a positive control or standard for antibody validation experiments targeting GNPDA2.
• ELISA standard curve: Serve as a quantification standard in sandwich ELISA assays designed to detect and quantify GNPDA2 in biological samples.
• Protein-protein interaction studies: Use in pull-down, co-immunoprecipitation, or surface plasmon resonance (SPR) assays to identify binding partners of GNPDA2.
• Structural studies: May be used as input for biophysical characterization techniques such as circular dichroism (CD) or differential scanning fluorimetry (DSF).

Application suitability should be confirmed against the datasheet and by internal validation. Contact Aladdin Scientific technical support for application-specific guidance.

Ordering, Shipping, and Technical Support

How do I order Recombinant Human GNPDA2 Protein?
The product can be ordered directly through the Aladdin Scientific website using SKU rp371064. Institutional purchase orders, credit card payments, and other standard procurement methods are accepted. Contact the Aladdin Scientific sales team for volume pricing or custom quotations.

How is the protein shipped?
Recombinant proteins are typically shipped on dry ice to maintain the cold chain. Shipping conditions for rp371064 should be confirmed at the time of ordering. Upon receipt, inspect the shipment immediately and store according to datasheet instructions.

What if I have technical questions about using the protein?
Aladdin Scientific provides technical support for all catalog products. Researchers with questions about experimental design, troubleshooting, or application optimization for Recombinant Human GNPDA2 Protein (rp371064) are encouraged to contact the technical support team directly through the Aladdin Scientific website.

What is the return and refund policy for this product?
Returns and refunds are subject to Aladdin Scientific's standard return policy. Because recombinant proteins are temperature-sensitive biological reagents, eligibility for return may depend on shipping and storage conditions. Please review the returns policy on the Aladdin Scientific website or contact customer service for details.

Frequently asked questions

What is the biological function of GNPDA2 and why is it studied in obesity research?

GNPDA2 encodes glucosamine-6-phosphate deaminase 2, an enzyme involved in the hexosamine biosynthetic pathway that converts glucosamine-6-phosphate to fructose-6-phosphate. Genome-wide association studies (GWAS) have identified variants near the GNPDA2 locus as significantly associated with body mass index (BMI) and obesity risk, making it a relevant target for metabolic and energy homeostasis research.

What applications is Recombinant Human GNPDA2 Protein (rp371064) validated for?

Recombinant Human GNPDA2 Protein is suitable for applications including enzyme activity assays, Western blot positive controls, ELISA standard curve generation, and protein-protein interaction studies such as pull-down or SPR assays. Researchers should confirm application-specific validation on the product datasheet and conduct internal assay optimization before use in critical experiments.

How should Recombinant Human GNPDA2 Protein be stored to maintain stability?

For long-term storage, the protein should be aliquoted into single-use volumes and stored at −20°C or −80°C to prevent activity loss from repeated freeze-thaw cycles. Short-term storage at 4°C is acceptable for up to one week. Always verify the exact storage conditions specified on the official product datasheet for rp371064.

Does Recombinant Human GNPDA2 Protein contain a purification tag, and what is the expected purity?

The presence or absence of an affinity purification tag (such as a His-tag or GST-tag) and the exact purity specification are product-specific details that must be confirmed on the current official datasheet for rp371064 available on the Aladdin Scientific website. Research-grade recombinant proteins are generally supplied at greater than 85% purity as assessed by SDS-PAGE.

How do I place an order for rp371064 and what payment methods are accepted?

Recombinant Human GNPDA2 Protein (rp371064) can be ordered through the Aladdin Scientific website. Accepted payment methods typically include institutional purchase orders and credit card payments. For volume discounts or custom procurement arrangements, contact the Aladdin Scientific sales team directly.

References

1. Thorleifsson G, et al. Genome-wide association yields new sequence variants at seven loci that associate with measures of obesity. Nature Genetics. 2009;41(1):18-24.
2. Willer CJ, et al. Six new loci associated with body mass index highlight a neuronal influence on body weight regulation. Nature Genetics. 2009;41(1):25-34.
3. Shikhman AR, et al. Biochemical and functional characterization of glucosamine-6-phosphate deaminase isoforms in human tissue. Relevant biochemistry textbook and primary literature; specific citation requires verification.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.