Recombinant Human GOLGA1 Protein: Key Facts & FAQs

Recombinant Human GOLGA1 Protein is a research-grade recombinant protein corresponding to the human Golgi autoantigen, golgin subfamily A member 1, produced for use in biochemical, cell biological, and immunological research applications.

Product Name

Recombinant Human GOLGA1 Protein

SKU

rp374274

Gene / Protein Target

GOLGA1 (Golgin subfamily A member 1; Golgi autoantigen)

Species

Human (Homo sapiens)

Protein Class

Golgin / Coiled-coil Golgi matrix protein

Primary Research Area

Golgi structure, vesicular trafficking, autoimmune research

What Is GOLGA1 and Why Is It Scientifically Important?

GOLGA1 (Golgi Autoantigen, Golgin Subfamily A Member 1), also known as golgin-97 in some literature contexts, is a member of the golgin family of coiled-coil proteins that are peripherally associated with the cytoplasmic face of Golgi membranes. Golgins play essential structural roles in maintaining the characteristic stacked architecture of the Golgi apparatus and in tethering transport vesicles to the correct Golgi compartment.

GOLGA1 was originally identified as an autoantigen recognized by autoantibodies in patients with certain systemic autoimmune diseases, making it relevant for both cell biology and clinical immunology research. Its coiled-coil domain structure facilitates protein–protein interactions critical for Golgi ribbon formation and membrane trafficking fidelity. Researchers study GOLGA1 to understand Golgi dynamics, secretory pathway regulation, and the molecular basis of autoimmune reactivity against Golgi antigens.

Product Specifications and Quality Attributes

Aladdin Scientific's Recombinant Human GOLGA1 Protein (SKU: rp374274) is produced using recombinant expression technology to ensure consistency and reproducibility across experiments. The following general quality attributes are typical for recombinant proteins of this class:

• Origin: Human (Homo sapiens)
• Expression System: Recombinant expression system (specific host system details should be confirmed with the product datasheet)
• Protein Class: Golgin / coiled-coil Golgi-associated protein
• Purity: Research-grade; specific purity grade should be confirmed from the current product datasheet
• Form: Lyophilized or liquid formulation; confirm with current datasheet
• Storage: Store according to datasheet instructions; typically at -20°C or -80°C to preserve activity

Note: Always refer to the official product datasheet for the most current and authoritative specifications before use.

Common Research Applications of Recombinant Human GOLGA1 Protein

Recombinant Human GOLGA1 Protein is suitable for multiple research applications leveraging its identity as a defined, purified antigen or functional protein:

• ELISA and Immunoassay Development: As a well-defined recombinant antigen, GOLGA1 protein can be used to develop or validate ELISA assays for detecting anti-GOLGA1 autoantibodies in patient sera, supporting autoimmune disease research.
• Antibody Production and Validation: Used as an immunogen or positive control to generate and validate antibodies targeting GOLGA1 epitopes.
• Western Blot and SDS-PAGE: Suitable as a positive control standard on Western blots to confirm antibody specificity.
• Protein Interaction Studies: Can be used in pull-down assays or co-immunoprecipitation experiments to identify GOLGA1-interacting partners involved in Golgi tethering complexes and vesicular transport.
• Structural and Biophysical Studies: Provides material for circular dichroism (CD), SEC, or other biophysical characterization of coiled-coil domain architecture.

Handling, Reconstitution, and Storage Guidelines

Proper handling of recombinant proteins is critical to maintain biological integrity and experimental reproducibility. The following general guidelines apply to Recombinant Human GOLGA1 Protein:

• Reconstitution: If supplied in lyophilized form, reconstitute using the volume and buffer specified on the product datasheet. Allow the vial to equilibrate to room temperature before opening, and gently reconstitute to avoid foaming.
• Aliquoting: After reconstitution, prepare single-use aliquots to minimize freeze-thaw cycles, which can degrade protein activity and cause aggregation.
• Storage Temperature: Store reconstituted protein at -20°C for short-term storage or -80°C for long-term storage. Avoid repeated freeze-thaw cycles.
• Carrier Protein: Some formulations include BSA or other stabilizers; confirm the exact formulation on the datasheet to ensure compatibility with your downstream assay.
• Expiry and Lot Tracking: Always record the lot number and expiry date for experimental traceability and compliance.

Consult the official product datasheet (SKU: rp374274) for product-specific reconstitution volumes, buffer composition, and validated storage conditions.

Ordering and Technical Support Information

Recombinant Human GOLGA1 Protein is available from Aladdin Scientific under SKU rp374274. To place an order or request additional technical information, customers may:

• Visit the Aladdin Scientific website and search by SKU rp374274 or product name.
• Contact Aladdin Scientific's technical support team for questions regarding protein specifications, custom formulations, or bulk pricing.
• Request a Safety Data Sheet (SDS) or Certificate of Analysis (CoA) for your specific lot through the customer service portal.
• Consult the product datasheet for validated application data and recommended assay conditions.

For returns, refunds, or shipping inquiries, please refer to Aladdin Scientific's standard policies available on the company website or contact the customer service team directly.

Frequently asked questions

What is Recombinant Human GOLGA1 Protein and what does GOLGA1 do in the cell?

GOLGA1 (Golgi Autoantigen, Golgin Subfamily A Member 1) is a coiled-coil protein that localizes to the cytoplasmic face of the Golgi apparatus. It functions as a structural component of the Golgi matrix, contributing to the maintenance of Golgi stack architecture and facilitating vesicular tethering and trafficking through the secretory pathway. Recombinant Human GOLGA1 Protein is the laboratory-produced form of this protein, used as a research reagent.

What research applications is Recombinant Human GOLGA1 Protein (rp374274) validated for?

Recombinant Human GOLGA1 Protein is generally suitable for applications including ELISA-based autoantibody detection, antibody production and validation (as immunogen or positive control), Western blot positive control, protein-protein interaction studies (pull-down, co-IP), and biophysical characterization of the coiled-coil domain. Specific validated applications for SKU rp374274 should be confirmed on the official product datasheet, as validated application data may vary by lot.

How should I store and handle Recombinant Human GOLGA1 Protein to maintain its activity?

As a general best practice for recombinant proteins, store Recombinant Human GOLGA1 Protein at -20°C for short-term use or at -80°C for long-term storage. After reconstitution (if lyophilized), prepare small single-use aliquots to avoid repeated freeze-thaw cycles, which can reduce protein activity and promote aggregation. Always follow the specific storage and reconstitution instructions provided on the product datasheet for SKU rp374274.

Why is GOLGA1 relevant to autoimmune disease research?

GOLGA1 was identified as a Golgi autoantigen because it is recognized by autoantibodies present in the sera of some patients with systemic autoimmune conditions. Detection of anti-Golgi autoantibodies, including those targeting GOLGA1, can have diagnostic and pathophysiological relevance. Recombinant GOLGA1 protein enables researchers to develop specific immunoassays for detecting and quantifying these autoantibodies in patient samples.

How can I obtain a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for rp374274?

Certificates of Analysis and Safety Data Sheets for Recombinant Human GOLGA1 Protein (rp374274) can be requested through Aladdin Scientific's customer service portal on the company website. When requesting a CoA, provide your product lot number to receive the document specific to your batch. For further assistance, contact Aladdin Scientific's customer support team directly.

References

1. Fritzler MJ, Hamel JC, Ochs RL, Chan EK. Molecular characterization of two human autoantigens: unique cDNAs encoding 95- and 160-kD proteins of a putative family in the Golgi complex. J Exp Med. 1993;178(1):49-62.
2. Barr FA, Short B. Golgins in the structure and dynamics of the Golgi apparatus. Curr Opin Cell Biol. 2003;15(4):405-413.
3. Aladdin Scientific. Recombinant Human GOLGA1 Protein, SKU rp374274. Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.