Recombinant Human LAMP3 Protein: Research Guide & Applications

Recombinant Human LAMP3 Protein is a research-grade recombinant form of the lysosomal-associated membrane protein 3, widely used as a mature dendritic cell marker and tool for investigating lysosomal trafficking, antigen presentation, and immune cell biology.

Protein Name

Recombinant Human LAMP3 Protein

SKU

rp365834

Also Known As

DC-LAMP, CD208, LAMP3

Protein Family

Lysosomal-Associated Membrane Protein (LAMP) family

Primary Research Areas

Lysosomal trafficking, dendritic cell maturation, antigen presentation, cancer biomarker research

Recommended Storage

Store at -20°C or -80°C; avoid repeated freeze-thaw cycles

Biological Background of LAMP3

LAMP3 (Lysosomal-Associated Membrane Protein 3), also designated CD208 and DC-LAMP, is a type I transmembrane glycoprotein and a member of the LAMP protein family. It is predominantly expressed in mature dendritic cells (DCs), where it localizes to lysosomes and MHC class II compartments. LAMP3 plays an important role in lysosomal membrane integrity, vesicular trafficking, and the regulation of MHC class II antigen presentation pathways.

Unlike the more ubiquitously expressed LAMP1 and LAMP2, LAMP3 expression is tightly restricted to mature dendritic cells in normal physiological conditions, making it a highly selective marker for DC maturation. LAMP3 has also been reported to be upregulated in several tumor types, suggesting roles in cancer progression and potential utility as a prognostic biomarker.

Product Overview and Specifications

Aladdin Scientific's Recombinant Human LAMP3 Protein (SKU: rp365834) is produced to research-grade quality standards. The recombinant protein is designed to support a wide range of in vitro research applications including immunoassay development, functional studies, and use as a reference standard.

Researchers should consult the product datasheet for confirmed details on expression host system, tag configuration, molecular weight, purity grade, and formulation buffer, as these parameters are critical for experimental design. Contact Aladdin Scientific technical support for application-specific guidance.

Key specification categories to verify from the datasheet:

• Expression host (e.g., E. coli, HEK293, CHO, baculovirus)
• Tag type and position (e.g., His-tag, Fc-fusion)
• Purity (typically ≥90% by SDS-PAGE for research-grade proteins)
• Endotoxin levels (relevant for cell-based assays)
• Formulation and recommended reconstitution buffer

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling of recombinant proteins are essential to maintain biological activity and experimental reproducibility. The following are general best-practice guidelines for recombinant proteins of this class; always defer to the specific product datasheet for confirmed conditions:

• Short-term storage: Store at 4°C for up to 1 week if the protein will be used promptly after receipt.
• Long-term storage: Aliquot and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles, which can lead to aggregation and loss of activity.
• Carrier protein: For highly dilute solutions, consider adding a carrier protein such as 0.1% BSA to minimize adsorption to tube surfaces.
• Reconstitution: If supplied lyophilized, reconstitute with sterile deionized water or the buffer recommended in the datasheet. Allow the vial to equilibrate to room temperature before opening. Gently mix; do not vortex.
• Working aliquots: Prepare single-use aliquots to avoid repeated freeze-thaw cycles after reconstitution.

Research Applications

Recombinant Human LAMP3 Protein supports a broad range of research applications in immunology, cell biology, and oncology:

• Dendritic cell biology: Used as a reference antigen to study DC maturation markers, signaling pathways, and functional responses. LAMP3 positivity distinguishes mature DCs from immature precursors.
• Antigen presentation research: LAMP3's association with MHC class II compartments makes recombinant LAMP3 useful for investigating lysosomal processing and antigen loading mechanisms.
• Immunoassay development: Suitable as a coating antigen or positive control in ELISA, lateral flow, and other immunoassay platforms for antibody screening and validation.
• Cancer biomarker studies: LAMP3 overexpression has been observed in certain cancers. Recombinant LAMP3 can be used in western blot, ELISA, and cell-free binding assays to validate antibodies and study protein interactions.
• Protein interaction studies: Surface plasmon resonance (SPR), co-immunoprecipitation, and pull-down assays to identify LAMP3-interacting partners.

Quality Assurance and Technical Support

Aladdin Scientific is committed to providing high-quality research reagents supported by rigorous quality control. Each lot of Recombinant Human LAMP3 Protein undergoes quality testing; consult the certificate of analysis (CoA) for lot-specific data including purity, concentration, and activity verification results.

For technical questions regarding experimental design, application optimization, or troubleshooting, contact Aladdin Scientific's technical support team. When ordering, reference SKU rp365834 to ensure you receive the correct product.

Researchers are encouraged to cite the product (manufacturer, catalog number, and lot number) in publications to enable full experimental reproducibility.

Frequently asked questions

What is LAMP3 and why is it significant in immunology research?

LAMP3 (CD208/DC-LAMP) is a lysosomal membrane glycoprotein selectively expressed in mature dendritic cells, where it plays roles in lysosomal trafficking and MHC class II antigen presentation. Its restricted expression pattern makes it one of the most specific markers for mature DC identification and functional studies in both healthy and disease contexts.

How should I store Recombinant Human LAMP3 Protein to maintain activity?

For long-term storage, aliquot the protein and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles as these can cause aggregation and reduce biological activity. If the protein will be used within one week of receipt, short-term storage at 4°C is acceptable. Always refer to the product datasheet for lot-specific storage instructions.

What applications is Recombinant Human LAMP3 Protein (rp365834) suitable for?

Recombinant Human LAMP3 Protein is suitable for multiple research applications including ELISA (as coating antigen or positive control), western blot validation, protein-protein interaction studies (SPR, co-IP, pull-down), and immunoassay development. It is also used in dendritic cell biology and cancer biomarker research. Confirm specific validated applications in the product datasheet before use.

Is the recombinant LAMP3 protein suitable for cell-based functional assays?

Suitability for cell-based assays depends on the expression host and endotoxin levels of the specific lot. Proteins expressed in mammalian systems generally carry more native-like glycosylation. Low endotoxin specifications are critical for cell-based work. Review the certificate of analysis and datasheet for these parameters, or contact Aladdin Scientific technical support for guidance.

References

1. de Saint-Vis B, et al. A novel lysosome-associated membrane glycoprotein, DC-LAMP, induced upon DC maturation, is transiently expressed in MHC class II compartment. Immunity. 1998;9(3):325-336. PMID:9768752
2. Wilke CM, et al. LAMP3 expression in dendritic cells and tumor cells. Review of literature; details require independent verification.
3. Aladdin Scientific. Recombinant Human LAMP3 Protein, SKU rp365834. Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.