Recombinant Human LST1 Protein: Research Guide & Applications

Recombinant Human LST1 Protein is a research-grade reagent targeting the leukocyte-specific transcript 1 (LST1) gene product, an MHC class III region-encoded transmembrane protein implicated in immune cell regulation and natural killer cell function.

Product Name

Recombinant Human LST1 Protein

SKU

rp369334

Gene Name

LST1 (Leukocyte Specific Transcript 1)

Protein Origin

Human (Homo sapiens)

Chromosomal Location

MHC class III region, chromosome 6p21.3

Protein Type

Transmembrane protein; multiple splice variants reported

Primary Research Areas

Immunology, NK cell biology, innate immunity, MHC biology

Background: LST1 Gene and Protein Biology

LST1 (Leukocyte Specific Transcript 1) is encoded within the major histocompatibility complex (MHC) class III region on human chromosome 6p21.3, one of the most gene-dense and immunologically relevant regions of the human genome. The LST1 gene gives rise to a remarkable diversity of transcript variants through alternative splicing, producing both transmembrane and soluble protein isoforms. Expression of LST1 is predominantly restricted to cells of the hematopoietic lineage, including monocytes, dendritic cells, and natural killer (NK) cells, suggesting a specialized role in innate immune regulation.

The LST1 protein contains short cytoplasmic tails and one or more transmembrane domains depending on the isoform. Studies indicate that LST1 plays a role in modulating immune cell morphology, promoting the formation of membrane protrusions, and influencing the organization of the immunological synapse. Its restricted leukocyte expression and location within the MHC locus have made it a candidate gene in susceptibility studies for autoimmune and inflammatory diseases.

Product Description and Key Specifications

Aladdin Scientific's Recombinant Human LST1 Protein (SKU: rp369334) is produced to research-grade standards, providing a defined and reproducible source of LST1 protein for in vitro studies. Recombinant expression systems allow researchers to obtain quantities of LST1 protein that would be impractical to isolate from primary leukocyte populations, enabling biochemical characterization, antibody validation, binding assays, and functional studies.

Researchers should consult the product datasheet for lot-specific information including host expression system, protein tag (if any), purity grade, formulation buffer, recommended storage conditions, and concentration. Key parameters to verify upon receipt include protein integrity by SDS-PAGE or western blot and biological activity where an activity assay is specified. All specific numerical values such as purity percentage, endotoxin levels, and concentration should be referenced from the current certificate of analysis (CoA) provided with each lot.

Recommended Applications and Experimental Use

Recombinant Human LST1 Protein is suitable for a range of research applications in immunology and cell biology. Common uses include:

• Antibody Production and Validation: LST1 recombinant protein serves as an antigen for generating and validating anti-LST1 antibodies for use in western blotting, immunofluorescence, flow cytometry, and immunohistochemistry.
• Binding and Interaction Studies: The protein can be used in pull-down assays, co-immunoprecipitation controls, or surface plasmon resonance (SPR) experiments to identify LST1-interacting partners.
• Functional Immunological Assays: Researchers studying NK cell biology, innate immune signaling, or MHC class III-associated pathways may use recombinant LST1 as a reference or stimulatory reagent.
• Structural Studies: Recombinant LST1 protein can support biophysical characterization of its transmembrane domains and isoform-specific structural features.
• Disease Research: Given LST1's mapping to the MHC locus, it is relevant to research into autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, and other inflammatory conditions where MHC region genes have been implicated.

Always optimize working concentrations empirically for each experimental system. Refer to the product datasheet for application-specific recommendations.

Handling, Storage, and Reconstitution Guidelines

Proper handling of recombinant proteins is critical to maintaining activity and integrity. The following general guidelines apply to Recombinant Human LST1 Protein; always defer to the specific instructions on the product datasheet and CoA supplied with your lot.

• Storage: Store lyophilized or liquid protein preparations according to the temperature specified on the datasheet (typically –20°C or –80°C for long-term storage). Avoid repeated freeze-thaw cycles, which can reduce protein activity and cause aggregation.
• Reconstitution: If supplied in lyophilized form, reconstitute with the volume and buffer type specified in the datasheet. Allow the vial to equilibrate to room temperature before opening. Gently mix without vortexing to avoid denaturation.
• Aliquoting: Upon first use, prepare single-use aliquots to minimize freeze-thaw cycles. Store aliquots at –80°C when not in immediate use.
• Carrier Protein: For dilute working solutions, consider adding a carrier protein such as BSA (0.1–0.5%) to prevent adsorption to tube surfaces and maintain protein stability.
• Quality Check: Confirm protein integrity by SDS-PAGE and, where applicable, verify biological activity with a functional assay before use in critical experiments.

Relevance to Autoimmune and Inflammatory Disease Research

The LST1 gene resides within the MHC class III region, a chromosomal segment with well-established associations to a broad spectrum of autoimmune and inflammatory diseases. Genome-wide association studies (GWAS) and fine-mapping analyses of the MHC locus have highlighted multiple candidate genes in this region, and LST1 has been of interest due to its immune-restricted expression pattern and potential roles in modulating leukocyte behavior.

Research into LST1 is relevant for scientists investigating the molecular underpinnings of conditions such as rheumatoid arthritis, systemic lupus erythematosus, type 1 diabetes, and multiple sclerosis, where MHC region variants contribute to disease risk. Recombinant Human LST1 Protein provides a tractable tool for in vitro dissection of LST1 function in immune cells, aiding hypothesis testing in disease-relevant cellular models. Users are encouraged to review current literature for the latest findings on LST1's mechanistic roles and disease associations before designing experiments.

Frequently asked questions

What is LST1 and why is it important in immunology research?

LST1 (Leukocyte Specific Transcript 1) is a transmembrane protein encoded in the MHC class III region of chromosome 6p21.3. It is expressed predominantly in leukocytes, including monocytes, dendritic cells, and NK cells, and is implicated in immune cell morphology regulation and innate immune signaling. Its location in the MHC locus makes it a candidate gene in autoimmune and inflammatory disease research.

What applications is Recombinant Human LST1 Protein (rp369334) suitable for?

Recombinant Human LST1 Protein is suitable for antibody production and validation, protein-protein interaction studies (e.g., pull-down, SPR), functional immunological assays, structural characterization of transmembrane isoforms, and as a research tool in autoimmune disease models. Refer to the product datasheet for application-specific guidance and recommended working concentrations.

How should Recombinant Human LST1 Protein be stored and handled to maintain its integrity?

Store the protein according to the temperature specified on the datasheet, typically at –20°C or –80°C for long-term storage. Avoid repeated freeze-thaw cycles by preparing single-use aliquots upon first use. For reconstitution of lyophilized preparations, follow the datasheet instructions carefully and mix gently without vortexing. Adding a carrier protein such as 0.1–0.5% BSA to dilute solutions can help maintain stability.

Does LST1 have multiple isoforms, and does this affect my experimental design?

Yes, LST1 is known to produce multiple transcript variants through alternative splicing, yielding both transmembrane and soluble protein isoforms. When designing experiments, it is important to confirm which isoform is represented by the recombinant protein (rp369334) by consulting the product datasheet, and to consider isoform-specific antibodies or detection methods to ensure experimental specificity.

Where can I find lot-specific purity, endotoxin, and activity data for rp369334?

Lot-specific quality data including purity (typically determined by SDS-PAGE or HPLC), endotoxin levels, and biological activity (where applicable) are provided on the Certificate of Analysis (CoA) supplied with each shipment. You may also request the CoA from Aladdin Scientific customer service or download it from the product page on the Aladdin Scientific website using your lot number.

References

1. Holzinger I, et al. Identification of the leukocyte-specific transcript LST1 within the MHC class III region. Immunogenetics. 1995.
2. Rollinger-Holzinger I, et al. LST1: a gene with extensive alternative splicing and immunomodulatory function. J Immunol. 2000.
3. MHC Sequencing Consortium. Complete sequence and gene map of a human major histocompatibility complex. Nature. 1999.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.