Recombinant Human MECOM Protein: FAQs & Key Facts

Recombinant Human MECOM Protein is a research-grade recombinant form of the MDS1 and EVI1 complex locus protein, a zinc-finger transcription factor critical to hematopoietic stem cell maintenance and implicated in myeloid malignancies.

Product Name

Recombinant Human MECOM Protein

SKU

rp369520

Protein Family

Zinc-finger transcription factor (EVI1/MDS1-EVI1 family)

Species Origin

Human (Homo sapiens)

Primary Research Areas

Hematopoiesis, leukemia/myeloid malignancy, stem cell biology, transcription regulation

Format

Recombinant protein; refer to product datasheet for expression system and tag details

What Is MECOM and Why Is It Important?

MECOM (MDS1 and EVI1 Complex Locus) encodes a family of zinc-finger transcription factors that includes the EVI1 and MDS1-EVI1 isoforms. These proteins play essential roles in the regulation of hematopoietic stem cell (HSC) self-renewal and differentiation. MECOM is located on human chromosome 3q26 and is recognized as a proto-oncogene; aberrant overexpression or chromosomal rearrangements involving the MECOM locus are frequently observed in acute myeloid leukemia (AML), myelodysplastic syndromes (MDS), and other hematologic malignancies. Because of its dual role as a master regulator of normal hematopoiesis and a driver of leukemic transformation, MECOM is an intensively studied target in both basic and translational research.

Product Description and Specifications

Recombinant Human MECOM Protein (SKU: rp369520) is produced to research-grade standards and is intended for use in biochemical and cell-based assays. The recombinant protein represents the human MECOM gene product and is designed to support studies on transcription factor activity, protein–protein interactions, and leukemia biology. For complete specifications—including the expression host system, protein tag, molecular weight, purity percentage, formulation buffer, and endotoxin levels—researchers should consult the official product datasheet available on the Aladdin Scientific website or contact technical support. All lot-specific quality control data are provided with each shipment.

Note: Specific numerical values for purity and other quality attributes are not reproduced here to avoid inaccuracy; always verify against the current certificate of analysis (CoA).

Storage, Handling, and Stability

Proper storage and handling are critical to maintaining the activity and integrity of recombinant proteins. As a general best practice for recombinant transcription factors:

• Store the protein at the temperature specified on the product datasheet (typically −20 °C or −80 °C for long-term storage).
• Avoid repeated freeze–thaw cycles, which can lead to aggregation and loss of activity. Aliquoting upon first thaw is strongly recommended.
• Keep the protein on ice during experimental setup to minimize degradation.
• Consult the specific datasheet for recommended working concentrations, compatible buffers, and shelf-life information.

For any storage conditions not explicitly covered in the datasheet, contact Aladdin Scientific technical support for guidance.

Recommended Research Applications

Recombinant Human MECOM Protein is suitable for a range of in vitro research applications, including but not limited to:

• Biochemical assays: Studying MECOM's DNA-binding activity and interactions with co-regulators.
• Protein–protein interaction studies: Co-immunoprecipitation, pull-down assays, and surface plasmon resonance to map MECOM interaction networks.
• ELISA and Western blot standards: As a positive control or calibration standard in immunodetection assays.
• Structural biology: Potential use in structural studies of zinc-finger domains (verify suitability with technical support).
• Drug discovery: Screening for small-molecule inhibitors that disrupt MECOM's transcriptional activity in leukemia models.

Application suitability should be confirmed by consulting the product datasheet and, where necessary, by conducting pilot experiments in your specific system.

Ordering and Technical Support

Recombinant Human MECOM Protein is available from Aladdin Scientific under SKU rp369520. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative. For technical questions regarding experimental design, optimal working concentrations, or troubleshooting, please reach out to Aladdin Scientific's technical support team, who can provide guidance based on your specific application requirements.

Bulk quantities and custom formulations may be available upon request. Certificate of Analysis (CoA) documents for each lot can be obtained from the product page or by contacting customer service.

Frequently asked questions

What is the MECOM protein and what does it do biologically?

MECOM (MDS1 and EVI1 Complex Locus) encodes zinc-finger transcription factors, including EVI1 and MDS1-EVI1 isoforms, that are master regulators of hematopoietic stem cell self-renewal and differentiation. Dysregulation of MECOM, particularly overexpression or chromosomal rearrangements at the 3q26 locus, is strongly associated with acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS).

What applications is Recombinant Human MECOM Protein (rp369520) validated for?

Recombinant Human MECOM Protein is intended for research use in biochemical assays, protein–protein interaction studies (e.g., co-immunoprecipitation and pull-down), immunodetection standards (ELISA, Western blot), and drug discovery screening. Researchers should consult the product datasheet for validated applications and confirm suitability in their own experimental systems through pilot experiments.

How should Recombinant Human MECOM Protein be stored to preserve its activity?

For long-term storage, recombinant proteins such as MECOM should generally be stored at −20 °C or −80 °C as specified on the product datasheet. Repeated freeze–thaw cycles should be avoided by aliquoting the protein upon first thaw. During experiments, keep the protein on ice. Always refer to the specific datasheet and certificate of analysis for lot-specific storage recommendations.

Is Recombinant Human MECOM Protein suitable for in vivo use?

Recombinant Human MECOM Protein (rp369520) is intended for research use only and is not approved for in vivo diagnostic or therapeutic applications. Endotoxin levels and formulation details relevant to cell-based or in vivo studies should be confirmed in the product datasheet and certificate of analysis before experimental use.

How do I obtain a Certificate of Analysis or Safety Data Sheet for this product?

The Certificate of Analysis (CoA) for each lot of Recombinant Human MECOM Protein can be downloaded from the Aladdin Scientific product page using the specific lot number, or requested through customer service. Safety Data Sheets (SDS) are also available on the product page or upon request from the Aladdin Scientific team.

References

1. Goyama S, Kurokawa M. Pathogenetic significance of Ecotropic viral integration site-1 (EVI1) in human myeloid malignancies. Cancer Science. 2010.
2. Bard-Chapeau EA, et al. EVI1 oncoprotein functions as a master regulator of multiple cellular processes including hematopoiesis. Cancer Research. 2013.
3. Aladdin Scientific. Recombinant Human MECOM Protein (rp369520) Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.