Dissolve 21.02 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. IDispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Component
g/liter
Agar
6
Cysteine Hydrochloride
10mg
Nutrient Broth
10
Sodium Chloride
5
Thymine
10mg
Total: 21.02g/Liter
500g makes 23.8 liters
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
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