Onion pollen mother cell smear and observation experiments
To understand the process of microspore formation and the changes of chromosomes in meiosis through the preparation of plant pollen mother cells, and to further
Topic
Genetic experiment
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
55 resources · protocols, FAQs & articles
Chromosome preparation experiments in the salivary gland of Drosophila melanogaster
Techniques for stripping salivary glands of larvae such as Drosophila and methods for making salivary chromosome specimens, and observations for characterizing
Bifactorial experiments in Drosophila
Through experiments to properly understand the essence of the law of segregation, to master the hybridization technique of Drosophila, and to learn to record th
Micronucleus Detection Experiment
The MicronucleusTest was developed in the early 1970s as a rapid and simple method of detecting environmental carcinogens. Micronuclei are the remnants of chrom
Preparation of mitotic chromosome specimens from onion root tips and observation experiments
Through the preparation and observation of plant root tips, the preliminary learning of plant tissues, cells, fixation, dissociation and compression methods, to
Experiments on companion inheritance in Drosophila
Through this experiment to correctly recognize the difference between orthogamy and transgamy of companion inheritance, and to master the characteristics of com
Sister chromatid differentiation staining experiment
Sister Chromatid Differential Staining (SCD) is a technique developed in the 1970s that allows the two chromatids of the midtelomeric chromosome to be colored d
Computer simulations of genetic tests
To understand the principles and methods of simulating genetics experiments by computer. Through the simulation of the laws of free association and chain exchan
Chromosome specimen preparation of mouse bone marrow cells
This lab provides an understanding of how mammalian chromosome specimens are made and observations of animal chromosomes. Source: Laboratory Course in Genetics
Morphology and life cycle of the fruit fly and its feeding experiments
Drosophila has a short life history, high reproduction rate, easy rearing and other characteristics, is a good material for the study of genetics. Through this
Experiments on quantitative traits in Drosophila
Drosophila quantitative trait experiments can be applied to: (1) characterize the inheritance of quantitative traits; and (2) learn to estimate inheritance.
Three-point assay in Drosophila
This experiment requires students to master the principles and methods of plotting genetics graphs, learn how to process data for experimental results, and furt
Experimental detection of micronuclei in the root tip of fava bean
This experiment will help you to understand the various types and principles of chromosome aberrations and to understand the general methods of monitoring envir
Mutagenic effect of nitrosoguanidine and screening experiments with nutrient-deficient strains of bacteria
Wild-type strains obtained from nature are generally able to grow on basic medium. After physical or chemical treatment, some of the wild-type cells have mutate
Non-isotopic labeling of DNA probes
DNA probes are divided into two categories: isotope-labeled probes and non-isotope-labeled probes. Isotope-labeled probes usually have high radioactivity and hi
Isolation and purification of chloroplast DNA from plant cells
Chloroplasts are unique organelles in plant cells and contain DNA. chloroplast DNA is in the form of a ring, and the chloroplast DNA (cpDNA) of higher plants va
Culture of human peripheral blood lymphocytes and chromosome preparation experiments
Source: Laboratory Course in Genetics
Bacterial splicing and gene localization-disrupted hybridization
It is a method of range localization by physically interrupting the binding action of bacteria.
Extraction of mitochondrial DNA from plant cells
In eukaryotes, in addition to the nuclear genome, there are cytoplasmic genomes, i.e., the mitochondrial genome and, in green plants, the chloroplast genome. Bo
Fluorescent genotyping screening for tiny subtelomeric rearrangement assay
Our results strongly support the notion that microtelomeric rearrangements play a very important role in spontaneous mental retardation (approximately 12% in ou
Human Chromosome Profiling Experiment
Source: Laboratory Course in Genetics
Plant mitotic chromosome pressing experiment
Conventional pressing techniques for plant chromosomes can be used to observe plant chromosomes commonly used.
Experiments in the culture and preparation of samples of pregnancy products and other solid tissue sources for chromosome analysis
In clinical practice, chromosomal studies require the use of solid tissues other than tumor tissue for one of two reasons: some patients can only obtain solid t
Liposomal vectors for direct in vivo gene transfection experiments
Many of the cationic liposomes used for liposomal transfection of cells in tissue cultures are able to be purchased. Composition optimization is highly dependen
Gene Complementation Experiment
Genes are the smallest functional units of biological genetic material, and genes are separable. To determine the boundaries of a gene, it is not possible to re
Deletion localization-gene fine structure analysis
Deletion localization is a gene localization method in which a strain at the mutation position to be tested is recombined separately with a series of mutant str
Fluorescence in situ hybridization of multicolor fibers
Source : Molecular Cytogenetics Techniques and Applications
Detection of recessive mutations in the X chromosome of Drosophila melanogaster
Mutation is a very common biological phenomenon, but the detection of mutation is a complex and tedious task, especially for higher plants and animals with larg
Comparative genomic hybridization (CGH) experiments on microarray mid-split phase chromosomes
The unique advantage of chromosomal CGH is its whole-gene screening capability, which is significantly faster and less labor-intensive than low-throughput metho
Point mutations are not easily amplified mutation system (ARMS) analysis experiments
The ARMS technique is based on the observation of the accessory oligonucleotide strand of a given DNA sequence, with the exception of a mismatch at the 3' end t
Plant polyploid artificial induction experiment
Plant polyploids are plants that have more than three sets of chromosome sets per cell. Common plants in nature are mostly diploid, and research has found some
Nuclease digestion of purified genomic DNA by Micrococcus spp.
MNase can cut DNA non-randomly. therefore, to determine how this naturally non-random pattern is affected by nuclear proteins such as histones, it is important
Non-Programmed DNA Synthesis Detection Experiment
Unscheduled DNA Synthesis (UDS) is DNA synthesis outside of S phase. In general, intracellular DNA synthesis is only seen in S phase, but when the cellular DNA
chromatin immunoprecipitation
Chromatin immunoprecipitation (ChIP) is a technique for studying protein-DNA interactions in vivo. The genomic DNA of eukaryotes exists in the form of chromatin
Chromosome gene localization experiments
Chromosomes are entities containing genes, which are only visible under the microscope as a general structure. Genes are nucleotide sequences at the molecular l
Experiments on the effect of environment on gene expression in Drosophila
Many aspects of phenotype are influenced by the genetic composition of an organism and the environment in which it lives, so it can be said that phenotype is a
Experiments to identify chromatin-bound proteins from total cell extracts by immunoprecipitation methods
This experiment is both a simple and very sensitive method for determining whether a known protein in Saccharomyces cerevisiae binds to a known DNA sequence. So
Meiosis mapping experiment
Currently, genetic mapping techniques can be used as a way to monitor the means of genomic manipulation, which needs to be realized through the use of molecular
Experimental molecular detection of uniparental diploids
Uniparental diploid (UPD) cells can have a normal cytogenetic karyotype but an imbalance in parental contributions. The diagnosis of uniparental diploidy involv
Experimental application of fluorescence in situ hybridization in the preimplantation diagnosis of aneuploidy
Pre-implantation Genetic Diagnosis (PGD), as a major new method of preventing genetic disorders, avoids the need for prenatal diagnosis and the possibility of t
Multiplex telomere fluorescence in situ hybridization assay
The multiple telomere FISH technique described in this chapter is the Chromoprobe Multiprobe-T system developed by Cytocell, which uses a microscope slide divid
Experiments on the preparation of chromosome smears in the mid-division of chorionic specimens
The interstitial center of the villi includes a range of cellular components-fibroblasts, endothelial cells, and macrophages-as well as a large amount of interc
High-throughput genotyping experiments using the TAQMAN assay
This unit describes the application of 5'-nuclease allele specific analysis or TaqMan method for single nucleotide polymorphism (SNP) typing.
Typing experiments with autofluorescence
The typing methods described in this unit are needed when there are a large number of samples with multiple microsatellite markers to be typed. For example, a d
Chromosome specimen preparation experiments for in vitro cultured cells
The source of this experiment is the official website of the Fourth Military Medical University
Observational experiments on Bartlett's microsomes in human cells
M.L. Barr et al. were the first to find a darkly stained vesicle in the interphase nucleus of nerve cells in female cats, but not in male cats. Since this vesic
Experiments on the preparation of plant chromosome specimens by the de-walled hypotonic method
Source: Laboratory Course in Genetics
Sequential four-molecule analysis experiments on Chlamydomonas reinhardtii
Neurosporacrassa belongs to the Ascomycetes class of fungi and is a good material for sequential tetramolecular analysis. The mycelium of Neurosporacrassa is ha
Chromatin immunoprecipitation sequencing (chip-seq)
Chromatin immunoprecipitation assay (ChIP) is currently the only method to study DNA-protein interactions in vivo, which mainly includes ChIP-qPCR and ChIP-seq.
Experiments to describe chromosomal structural abnormalities by comparative genomic hybridization
Comparative Genomic Hybridization (CGH) is a molecular cytogenetic technique capable of characterizing, in a one-step genome-wide screening procedure, additions
Confined transduction experiments with λ phage
Lambda phage is a kind of mild phage. In the host cell of E. coli, its DNA can be integrated in the host chromosome and passed on to the offspring cells just li
Concurrent transduction and gene localization--a three-dot hybridization experiment
The distance between two genes can be measured by the number of recombinants in the progeny after two- and three-point crosses. The closer the distance between
Spectral chromosome karyotyping experiments
Throughout the history of clinical cytogenetics, G-banding has served as the "gold standard" for detecting total chromosomal abnormalities in clinical samples,
Diagnosis of Fanconi anemia (congenital myelodysplasia)--dioxobutane (dibutyl epoxide) detection assay
Fanconi anemia (FA) is an autosomal recessive syndrome characterized by progressive bone marrow failure and an increased risk of malignancies, particularly acut
Restriction endonuclease profiling of λ phage DNA
Lambda phage is a commonly used biological material in molecular genetics research, and the enzymatically fragmented segments of its DNA are often used as stand
Related topics
Techniques often explored alongside immunological experiments.