Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Trypsin (EC 3.4.21.4) is a serine endopeptidase that specifically hydrolyzes peptide bonds at the carboxyl side of lysine (Lys) or arginine (Arg) residues. As biopharmaceuticals such as vaccines, recombinant proteins, and cell therapy products demand higher safety and consistency, the use of ...
Cell counting with a hemocytometer is a cornerstone of in vitro and in vivo experiments. It improves the precision and reproducibility of your research and increases the reliability of your results.
Cryopreservation is a method of preserving cells at low temperatures in order to maintain cell activity and function for a long period of time. This method is of great significance for the long-term preservation of cell lines, experimental repeatability, the establishment of gene banks, and the ...
Procedure for early detection of apoptosis using Annexin V-FITC staining and optional propidium iodide ( PI ) .
Current research has identified a variety of protein hydrolases that can be used to dissociate cells, the most commonly used of which is trypsin, a member of the serine protease family. In the pancreas the precursor of trypsin is synthesised by trypsinogen and secreted as a component of the ...
Techniques often explored alongside immunological experiments.
