Protocols

Tissue Homogenization Buffer for ELISA

· Reviewed · Published Jun 9, 2025 · Updated Jul 27, 2023 · 8 min read

Recipe

Stock Solution:

1.Weigh out 0.025 grams of Aprotinin and Luepeptin and dissolve in 25ml of water

2.Mix and aliquot into 70ul single use aliquots

To prepare homogenization buffer:

1.Add 50ul of stock solution to 100ml of PBS

2.Sterilze by filtration

Final concentration is 0.5ug/ml

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Tissue Homogenization Buffer for ELISA" Aladdin Knowledge Base, updated Jul 27, 2023. https://www.aladdinsci.com/us_en/faqs/tissue-homogenization-buffer-for-elisa-en.html

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