Fluorescamine - ≥98% , CAS No.38183-12-9

CAS: 38183-12-9 Cat. No.: F109021 Molecular Weight: 278.26 Beilstein Registry Number: 921143 EC Number: 253-814-5

GRADE & PURITY ≥98%

Synonyms

AS-14756 | 4'-phenylspiro[2-benzofuran-3,2'-furan]-1,3'-dione | 4-Phenylspiro(furan-2(3H),1'(3'H)-isobenzofuran)-3,3'-dione | 4'-phenylspiro[2-benzofuran-3,2'-furan]-1,3'-dion | FLUORESCAMINE [MI] | EINECS 253-814-5 | Ro 20-7234 | BP-13091 | RO-20-7234 |

Storage

Argon charged,Room temperature

Shipped In

Normal

Overview

A non-fluorescent reagent that reacts easily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful in fluorometric assays of amino acids, protein, and proteolytic enzymes. Adequately blocks newly generated amino termini in protein sequence analyses.
Fluorescamine reacts with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Application：

Fluorescamine has been used as a stain to localize granular proteins on the surface of foxtail millet starch as well as protein in foxtail millet flour through confocal scanning laser microscopy (CSLM). It has also been used for photometric determination of para-aminobenzoic acid.
Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Specifications

Synonyms

AS-14756 | 4'-phenylspiro[2-benzofuran-3, 2'-furan]-1, 3'-dione | 4-Phenylspiro(furan-2(3H), 1'(3'H)-isobenzofuran)-3, 3'-dione | 4'-phenylspiro[2-benzofuran-3, 2'-furan]-1, 3'-dion | FLUORESCAMINE [MI] | EINECS 253-814-5 | Ro 20-7234 | BP-13091 | RO-20-7234 |

Specifications & Purity

≥98%

Storage

Argon charged, Room temperature

Shipped In

Normal

Purity

≥98%

Names and Identifiers

• Pubchem Sid: 504753562
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504753562
• Canonical Smiles: C1=CC=C(C=C1)C2=COC3(C2=O)C4=CC=CC=C4C(=O)O3
• IUPAC Name: 4'-phenylspiro[2-benzofuran-3,2'-furan]-1,3'-dione
• InChIKey: ZFKJVJIDPQDDFY-UHFFFAOYSA-N
• INCHI: 1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
• Isomeric SMILES: C1=CC=C(C=C1)C2=COC3(C2=O)C4=CC=CC=C4C(=O)O3
• WGK Germany: 3
• Molecular Weight: 278.26
• Beilstein: 921143
• Reaxy-Rn: 921143
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=921143&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Benzofurans
      • Subclass Benzofuranones

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Benzofurans
• Subclass: Benzofuranones
• Intermediate Tree Nodes: Not available
• Direct Parent: Benzofuranones
• Alternative Parents: Phthalides Isobenzofurans Ketals Furanones Benzene and substituted derivatives Vinylogous esters Lactones Ketones Carboxylic acid esters Oxacyclic compounds Monocarboxylic acids and derivatives Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Benzofuranone - Isobenzofuranone - Phthalide - Isocoumaran - Isobenzofuran - Ketal - Monocyclic benzene moiety - 3-furanone - Benzenoid - Dihydrofuran - Vinylogous ester - Carboxylic acid ester - Ketone - Lactone - Oxacycle - Acetal - Carboxylic acid derivative - Monocarboxylic acid or derivatives - Hydrocarbon derivative - Carbonyl group - Organic oxide - Organic oxygen compound - Organooxygen compound - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as benzofuranones. These are organic compounds containing a benzene ring fused to a furanone.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Soluble in water (partly), ethanol, chloroform, DMSO, and acetonitrile.
• Sensitivity: Hygroscopic
• Melt Point(°C): 154-155°C
• Molecular Weight: 278.260 g/mol
• XLogP3: 2.900
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 1
• Exact Mass: 278.058 Da
• Monoisotopic Mass: 278.058 Da
• Topological Polar Surface Area: 52.600 Ų
• Heavy Atom Count: 21
• Formal Charge: 0
• Complexity: 503.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 1
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

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