FT206 - ≥98% , CAS No.2278274-34-1

CAS: 2278274-34-1 Cat. No.: F648395 Formula: C₂₅H₂₉N₅OS Molecular Weight: 447.60 PubChem CID: 137480383

GRADE & PURITY ≥98%

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

FT206 is an inhibitor of carboxamides as ubiquitin-specific protrase extracted from patent WO 2020033707 A1, example 11-1

Form:Solid

IC50& Target:Carboxamides as ubiquitin-specific protrase

Specifications

Specifications & Purity

≥98%

Biochemical and Physiological Mechanisms

FT206 is an inhibitor of carboxamides as ubiquitin-specific protrase extracted from patent WO 2020033707 A1, example 11-1.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Action Type

INHIBITOR

Purity

≥98%

Names and Identifiers

Canonical Smiles

CC1=NC2=C(C=C1)C(=C(S2)C(=O)NC3CCC4=C(C3)C=CC(=C4)N5CC6CCC(C5)N6)N

IUPAC Name

3-amino-N-[(2S)-6-(3,8-diazabicyclo[3.2.1]octan-3-yl)-1,2,3,4-tetrahydronaphthalen-2-yl]-6-methylthieno[2,3-b]pyridine-2-carboxamide

InChIKey

UZKKSCQGVUDMFY-VIQWUECVSA-N

INCHI

1S/C25H29N5OS/c1-14-2-9-21-22(26)23(32-25(21)27-14)24(31)29-17-5-3-16-11-20(8-4-15(16)10-17)30-12-18-6-7-19(13-30)28-18/h2,4,8-9,11,17-19,28H,3,5-7,10,12-13,26H2,1H3,(H,29,31)/t17-,18?,19?/m0/s1

PubChem CID

137480383

Molecular Weight

447.60

Chemical and Physical Properties

• Solubility: DMSO : 150 mg/mL (335.12 mM; Need ultrasonic)

Application Protocols

No validated application protocols are provided in the Product Data for this item.

• Tested applications: Not specified for this item; refer to CoA/Spec Sheet.
• Recommended dilutions/concentrations: Not specified for this item; refer to CoA/Spec Sheet.
• Positive/negative controls: Not specified for this item; refer to CoA/Spec Sheet.

Please develop assay-specific protocols internally, including controls, dose–response design, and solvent tolerances appropriate for your biological system and detection modality.

Biological Roles

No specific biological target or mode of action is provided in the Product Data for FT206. The notes below describe how small molecules like this are generally used to interrogate biology (general guidance; not item-specific claims):

• Chemical probes/tools: Small molecules can modulate protein function (inhibition/activation/allosteric binding) to provide temporal control over pathways. They complement genetic perturbations and can reveal reversible, dose-dependent phenotypes.
• Pathways commonly studied: Ubiquitin–proteasome signaling, kinase cascades, GPCR signaling, epigenetic regulation, and metabolic enzymes, among others. Choice of assays and readouts should be driven by hypothesized targets and cellular context.
• Assay design: Pair phenotypic endpoints with target engagement metrics (e.g., thermal shift, pull-downs) and genetic orthogonal controls (knockout/overexpression) to attribute causality.
• Selectivity profiling: Use panels (biochemical or proteomic) to map off-target interactions; concentration-response curves across a panel provide on-/off-target windows for cellular use.
• Data quality: Confirm reproducibility across independent lots and after storage. Monitor compound integrity in assay media (time-course LC–MS) to ensure constant exposure.

Reminder: This product is for research use only. No medical, diagnostic, or therapeutic applications are implied.

Buffer Applications

This product is a small-molecule tool compound and is not itself a buffering reagent. Therefore, dedicated buffer system guidance is generally not applicable.

Practical notes for bioassays (general):

• Use biologically compatible buffers such as HEPES (pH 7.2–7.6), PBS (pH ~7.4), or Tris (pH 7–8) depending on your assay target.
• Maintain final DMSO ≤0.1–1% v/v to limit buffer/biology perturbation; match vehicle control to the highest solvent % used.
• Add nonionic detergent (0.01–0.05%) when aggregation is a concern in biochemical assays.
• Include BSA (0.1–0.5%) if nonspecific adsorption to plastics is suspected; verify that additives do not interfere with readouts.

Item-specific buffering parameters (pKa, solubility vs pH, stability in buffer) are not provided in the Product Data; consult CoA/Spec Sheet or generate empirical stability curves as needed.

Green Alternatives

Greenness considerations are more relevant to the choice of dissolution solvent and analytical workflow than to the compound itself.

• Dissolution media (general assessment):
  • DMSO: Widely accepted in life-science assays; relatively benign compared to chlorinated solvents; fully miscible with water; biodegradable but can carry solutes into the environment—dispose responsibly.
  • Ethanol/IPA: Renewable feedstock options exist; lower toxicity profile; volatility aids recovery but can limit solubility for hydrophobic scaffolds.
  • Propylene glycol, PEG 200/400: Higher boiling, lower volatility; potential for greener profiles in some contexts but assay interference must be assessed.

Small comparison (general):

• DMSO vs Ethanol: DMSO offers broader solvency and better stability for many compounds; ethanol is greener/volatile but may require higher percentages that disrupt assays.
• DMF/NMP: Effective but less favorable from a green perspective; avoid unless necessary and minimize volumes.

Operational practices:

• Prepare concentrated stocks to minimize total solvent volumes.
• Use microplate formats and sealed storage to reduce solvent loss and emissions.
• Implement waste segregation for organic solvents and aqueous DMSO mixtures; consider solvent recycling where infrastructure exists.

No greener “substitute compound” is applicable, as FT206 is itself the research analyte.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or clinical use is provided for this item. This product is supplied strictly for research use only.

Context for R&D (general, non-clinical):

• Reference standard / tool compound: May be used in discovery research to benchmark assay performance, explore SAR, or validate phenotypes in preclinical systems.
• Formulation screening (in vitro/in vivo research): If formulation studies are pursued in non-clinical models, typical vehicles include DMSO diluted with PEG 300/400, aqueous buffers, or lipid-based carriers. Such activities require internal safety assessments and are outside the scope of this catalog listing.

Regulatory note:

• Not for use in humans or animals for therapeutic or diagnostic purposes. No GMP, USP/EP, or ICH quality designation is claimed in the Product Data. For any regulated applications, source a qualified material with appropriate certification.

Physical Properties

Item-specific physicochemical data are not provided in the Product Data. Use the CoA/Spec Sheet for authoritative values.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• LogP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• pKa: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not applicable for solids; if liquid, not specified for this item; refer to CoA/Spec Sheet.
• UV–Vis characteristics: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/practice, not item-specific):

• Many drug-like small molecules are handled as off-white solids with limited aqueous solubility and are commonly dissolved in anhydrous DMSO to prepare concentrated stocks (e.g., 10–50 mM), then diluted into assay buffers.
• If photolability is unknown, store and handle under low light and use amber vials as a precaution.
• Hygroscopicity and polymorphism, when relevant, can affect apparent melting behavior and solubility; check the CoA for any notes.

Quality & Grades

Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Context for small-molecule screening compounds (general guidance):

• Screening/Library grade: Typically supplied at high chemical purity suitable for biochemical and cell-based assays. Verification by LC–MS and 1H NMR is common; some suppliers report HPLC purity (e.g., area %). UV cutoff and trace metal analyses are usually not relevant unless the compound is intended for photochemical or metal-catalyzed reactions.
• Impurities and assay impact: Trace reactive impurities can confound bioassay readouts (e.g., PAINS-like behavior or redox activity). Review the CoA for purity data and replicate key results with orthogonal assays.
• Batch traceability: Each lot should be traceable to analytical data (HPLC/LC–MS, NMR). For critical applications (SAR comparisons, reference standard use), request a copy of the CoA and retain aliquots for reanalysis.
• Stabilizers: Not specified for this item; refer to CoA/Spec Sheet. If present, stabilizers may influence UV/fluorescence assays or downstream chemistry.

Notes specific to this listing:

• The Product Data state research use only and −20°C storage. If your program requires enhanced characterization (water content by KF, residual solvents, salt form, or polymorph), please request these details prior to use.

Reaction & Applications

This listing is a research small molecule primarily intended for screening and chemical biology rather than as a synthetic reagent. Accordingly, “applications” refer to discovery assays and analytical characterization (general guidance):

• Biochemical assays: Use in enzyme or target-binding assays to generate IC50/Ki or KD values; implement counterscreens to exclude assay interference (e.g., redox cycling, aggregation). Include detergent (0.01–0.05% Tween-20/Triton X-100) when appropriate to reduce colloidal artifacts.
• Cell-based assays: Determine cellular activity via dose–response curves; monitor cytotoxicity (e.g., CellTiter-Glo) alongside target-relevant phenotypes. Keep final DMSO ≤0.1–0.5% v/v unless cells tolerate more.
• Target engagement (general): CETSA/thermal shift, DARTS, NanoBRET/bioluminescence resonance energy transfer, or PROTAC-adjacent readouts where applicable. Confirm on-target effects with genetic controls (knockdown/knockout rescue).
• ADME profiling (discovery stage): Evaluate solubility, microsomal stability, permeability (e.g., PAMPA/Caco-2), and plasma protein binding to understand exposure constraints in advanced studies.
• Analytical characterization: LC–MS, HRMS, 1H/13C NMR, and HPLC purity checks before and after storage to ensure integrity.

Note: No item-specific target, mechanism, or application claims are provided in the Product Data. The above are general best practices for small-molecule tool compounds.

Reaction Conditions

Dedicated reaction conditions are not applicable because FT206 is not offered as a reagent for named organic reactions in this catalog entry.

If your work involves custom synthesis or derivatization (general guidance only):

• Choose conditions based on the known functional groups of the scaffold (consult primary literature/CoA). Protect or avoid labile moieties (e.g., acid- or base-sensitive groups) as needed.
• Employ inert atmosphere and anhydrous solvents when manipulating moisture-sensitive motifs; monitor by TLC/LC–MS.
• Purify by preparative HPLC or flash chromatography using method development that considers potential coelution with closely related impurities and isomeric byproducts.

Because no structure-specific information is provided in the Product Data, no further reaction-condition recommendations can be made here.

Safety & Handling

Safety overview: Item-specific hazard classifications are not provided in the Product Data. Always consult the SDS for authoritative and up-to-date safety information.

• GHS classification: Not specified for this item; refer to SDS.
• Signal word / H-statements / Pictograms: Not specified for this item; refer to SDS.

Best practices (general laboratory guidance):

• PPE: Wear lab coat, safety glasses, and appropriate chemically resistant gloves (e.g., nitrile). Handle powders in a fume hood to minimize inhalation and dust exposure.

• Avoid exposure: Do not inhale dust, ingest, or allow skin/eye contact. Many bioactive small molecules can be harmful even at low doses. Solutions in DMSO can enhance dermal absorption—avoid skin contact.

• Storage incompatibilities: Keep separate from strong oxidizers and strong acids/bases unless compatibility is known. Avoid moisture for moisture-sensitive materials.

• Spill/accident response: Collect solids by gentle sweeping under fume hood; for solutions, absorb with inert material. Dispose of waste per institutional and local regulations.

• First aid (general): If inhaled—move to fresh air; if on skin—wash with soap/water; if in eyes—rinse with water for several minutes; if ingested—rinse mouth. Seek medical attention in all exposure cases and provide SDS.

• Storage conditions (from Product Data): Store at −20°C. Shipments are made on ice with ice pads. Allow cold shipments to equilibrate in a desiccated environment before opening to avoid condensation.

Solvent Selection

This product is a small-molecule library member rather than a process solvent. Solvent selection below focuses on dissolving the compound for screening and analytical work (general guidance; not item-specific unless noted).

• Primary stock solvent (typical):
  • DMSO (anhydrous): Preferred for many drug-like small molecules due to broad solvency and biological compatibility at low final %.
• Secondary options (case-dependent):
  • DMF, NMP, ethanol, methanol may dissolve certain scaffolds; confirm compatibility with your assay and materials.
• Aqueous work:
  • Prepare a concentrated DMSO stock (e.g., 10–50 mM) and dilute into buffer (HEPES, PBS, Tris) to achieve final DMSO typically ≤0.1–1% v/v, balancing solubility and assay tolerance.
• Practical tips:
  • Warm gently (≤37°C) and vortex/sonicate to aid dissolution; avoid prolonged heating.
  • Filter through 0.2 µm PTFE/RC syringe filters for particulate removal prior to sensitive assays or LC injection.
  • Evaluate precipitation after dilution (visual check, nephelometry, or light scattering) and adjust DMSO % if needed.

Comparison (general):

• DMSO: broad solvency, good assay tolerance; hygroscopic; can increase dermal absorption risk.
• Ethanol: volatile, compatible up to ~1–2% in many assays; narrower solvency window.
• DMF/NMP: strong solvency but less biocompatible; use sparingly and confirm assay tolerance.

Storage & Reconstitution

• Storage conditions (from Product Data): Store at −20°C. Product ships in an ice chest with ice pads. Avoid repeated freeze–thaw cycles.

• Stability: Not specified for this item; refer to CoA/Spec Sheet.

• Salt form/solvate: Not specified for this item; refer to CoA/Spec Sheet.

General reconstitution guidance (not item-specific):

• Allow container to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.
• Dissolve in anhydrous DMSO to prepare a concentrated stock (e.g., 10–50 mM, as solubility permits). Vortex and, if necessary, sonicate briefly. Avoid prolonged heating.
• Aliquot stocks into low-bind, amber vials/tubes to minimize adsorption and photodegradation risks; store aliquots at −20°C or −80°C for long-term use.
• Limit freeze–thaw to a single cycle; thaw on ice and keep working solutions at 2–8°C during use. Discard aliquots showing precipitation or discoloration.
• For assay use, dilute the DMSO stock into buffer/media to a final DMSO typically ≤0.1–1% v/v with matched vehicle controls.

Always consult the CoA/Spec Sheet and SDS for item-specific stability, solubility, and handling instructions.

Structure & Identity

Brief overview: FT206 (SKU F648395) is listed in our small-molecule/compound library category as a research-use small molecule. Item-specific structural identifiers are limited in the Product Data; consult the CoA/Spec Sheet for definitive structure information.

• Product identifiers (from Product Data):

  • SKU: F648395
  • Product Name: FT206
  • CAS: 2278274-34-1
  • PubChem CID: 137480383
  • InChIKey: 373202 (as provided)
  • SMILES: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

• Structural features:

  • Specific functional groups, ring systems, and stereochemistry are not provided in the Product Data. Refer to the CoA/Spec Sheet for a definitive structural depiction (2D skeletal formula) and any stereochemical annotations.

• 2D structure (verbal description):

  • Not specified in Product Data; a detailed 2D description requires the exact SMILES/InChI. Please consult the CoA/Spec Sheet or the referenced CID entry for a structure diagram.

• Identity assurance:

  • For rigorous work (e.g., screening, SAR, or reference standard use), we recommend confirming identity by orthogonal techniques (e.g., LC–MS, HRMS, 1H/13C NMR) against the CoA/Spec Sheet reference.

Synthetic Utility

FT206 is offered as a finished small molecule for screening and is not positioned as a general synthetic reagent or building block in this listing. Consequently, classical synthetic utility topics (e.g., use as a coupling partner, protecting group strategy, chiral auxiliary) do not typically apply.

General considerations (if used in chemistry workflows):

• If analogs are to be prepared, consult the primary literature or internal SAR plans for feasible scaffold modifications and substituent tolerance. Start from the disclosed core rather than from the finished compound to avoid low-yielding late-stage transformations.
• For analytical support of custom syntheses, use LC–MS and 1H/13C NMR to confirm identity/purity relative to the supplied reference.

Item-specific reactivity, functional group profile, and compatibility with common transformations are not provided in the Product Data; refer to the CoA/Spec Sheet or original literature for synthetic routes and building-block availability.

Target Specificity

No target, mode of action, or selectivity profile is provided in the Product Data for this item.

• Target/antigen: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• Clone/isotype (if antibody): Not applicable.

For research planning, determine target specificity using your own assays or consult peer-reviewed literature corresponding to the CAS/CID if appropriate to your project. Do not infer target based solely on product name.