GRF2 Mouse mAb - Primary antibody

Cat. No.: Ab106407

GRADE & PURITY See COA

Application

IF/ICC, IHC, WB

Host species

Mouse

Reactive species

Dog, Human, Monkey, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

OTI2F5

Conjugation

Unconjugated

Overview

Mouse anti Human GRF2 Antibody, Monoclonal (OTI2F5), could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

GRF2 Mouse mAb - Primary antibody

Synonyms

C3G antibody | C3G protein antibody | CRK SH3 binding GNRP antibody | CRK SH3-binding GNRP antibody | DKFZp781P1719 antibody | GRF 2 antibody | GRF2 antibody | Guanine nucleotide releasing factor 2 (specific for crk proto oncogene) antibody | Guanine nucl

Specifications & Purity

See COA

Biochemical and Physiological Mechanisms

Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS.

Host species

Mouse

Immunogen

Recombinant full length protein of human RAPGEF1 produced in HKE293T cell.

Positive Control

WB: HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 lysates. IHC: Human breast, colon adenocarcinoma, ovary adenocarcinama, pancreas, thyroid, thyroid carcinoma, prostate carcinoma tissues. ICC/IF: COS7 cells.

Conjugation

Unconjugated

SDS-PAGE

150 kDa

Purification Method

Protein A/G purified

Concentration

See COA

Storage and Shipping

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Application

• Application: Dilution info
• IHC:

  1/150

• WB:

  1/400-1/4000

• IF/ICC:

  1:100 ;

Associated Targets(Human)

RAPGEF1 Tbio Rap guanine nucleotide exchange factor 1 (0 Activities)

Discover Target: RAPGEF1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Frequently Asked Questions

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GRF2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.