Hyaluronidase, CAS No.37259-53-3

CAS: 37259-53-3 Cat. No.: H141272 EC Number: 253-430-8

GRADE & PURITY EnzymoPure™, ≥ 300 IU/mg, from bovine testes

Overview

Hyaluronidase is widely present in nature.In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards. 

Application

Hyaluronidase from Streptomyces hyalurolyticus has been used:in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue；as a component of digestion solution for the derivation of germline stem cells from testicular tissue.

Specifications

Product Name

Hyaluronidase, CAS No.37259-53-3

Synonyms

Hyaluronate Lyase

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ≥ 300 IU/mg, from bovine testes

Biochemical and Physiological Mechanisms

Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is

CAS

37259-53-3

Enzyme Commission Number

3.2.1.35

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥ 300 IU/mg, from bovine testes

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Unit definition

Under conditions of 37 ℃ and pH 5.3-5.7, one unit can cause a 0.330% change in transmittance of 2.0 mL reaction mixture at 600 nm (detected at 45 min)