Hydroxyl Radical Content Assay Kit (Micro Method)

Cat. No.: H1522192

GRADE & PURITY BioReagent

Overview

Hydroxyl radicals (OH⁻), as a key member of the reactive oxygen species (ROS) family, possess extremely strong oxidative capacity and serve as one of the critical indicators for assessing oxidative stress levels in living organisms. This product is specifically designed for the high-sensitivity detection of hydroxyl radical content in various biological samples, with a broad range of applications including precise analysis of animal and plant tissues, microorganisms (e.g., bacteria), cultured cells, serum/plasma, and various liquid samples. The detection principle of this kit is based on the unique oxidative properties of hydroxyl radicals: In the reaction system, 2-deoxyribose acts as a specific substrate and can be efficiently oxidized by OH⁻ to generate malondialdehyde analogues. This product then undergoes a specific condensation reaction with thiobarbituric acid (TBA), forming a stable pink compound with a maximum absorption peak at 532 nm. By measuring the absorbance of this colored product at 532 nm, a quantitative relationship with the hydroxyl radical content can be established, ultimately allowing for the calculation of the hydroxyl radical concentration in the sample.

Specifications

Specifications & Purity

BioReagent

Grade

BioReagent

Application

Cell Metabolism

Sample type

Animal tissues, Bacteria, Plant tissues, Plasma, Serum

Method Of Detection

Colorimetric Method

Detection instrument

Microplate reader

Detection wavelength

532nm

Storage and Shipping

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ for 6 months. Store in the dark.