GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Suitable for mass spectrometry (MS)?MS grade — low non-volatile and ionic residues compatible with mass spectrometry. Use to minimize background and ion suppression in MS workflows.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥99%(HPLC)≥50 U/μg protein
EnzymoPure™,suitable for mass spectrometry (MS),Recombinant,≥99%(HPLC),≥50 U/μg protein Recombinant,Suitable for mass spectrometry (MS),EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
IdeS (IgG-specific degrading enzyme) is an engineered protease recombinantly expressed in Escherichia coli.
It specifically cleaves immunoglobulin G at a single recognition site below the hinge region to generate homogeneous F(ab´)₂ and Fc fragments.
Both IdeS and IdeZ proteases efficiently cleave human IgG1, IgG2, IgG3, IgG4, monkey, sheep, rabbit, humanized and chimeric IgG antibodies, as well as Fc fusion proteins.
However, only IdeZ protease cleaves mouse IgG2a and IgG3.
Reconstitution:
Reconstitute with 40 μL of ultrapure water.
Digestion Buffer:
10× buffer containing 50 mM PBS, 150 mM NaCl (pH 6.6).
pH Range:
Optimal activity at pH 6–8.
Application:
· IdeS protease is an IgG-specific protease that generates homogeneous F(ab´)₂ and Fc fragments.
· Recommended Digestion SOP:
· Take 10 μL of mAb sample at 10 μg/μL;
· Add 5 μL of 10× digestion buffer and 31 μL of ultrapure water, mix to a total reaction volume of 50 μL;
· Add 4 μL of rIdeS protease at 0.5 μg/μL;
· Incubate at 37 °C for 30 minutes.
Specifications
Product Name
IdeS Protease (MS)
Grade
Recombinant, Suitable for mass spectrometry (MS), EnzymoPure™
Specifications & Purity
EnzymoPure™,suitable for mass spectrometry (MS),Recombinant,≥99%(HPLC),≥50 U/μg protein
Molecule Type
Enzyme
Storage and Shipping
Concentration
≥50 U/μg protein
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Unit definition
The amount of IdeS protease required to completely cleave 50 μg of IgG antibody within 30 minutes at 37 °C.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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