Insulin solution human, CAS No.11061-68-0

CAS: 11061-68-0 Cat. No.: I755758 Formula: C₂₅₇H₃₈₃N₆₅O₇₇S₆ Molecular Weight: 5807.57 EC Number: 234-279-7

GRADE & PURITY BioReagent, 10mg/ml

Overview

Insulin is a two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond.

Application

Insulin solution human has been used as a supplement in Dulbecco′s modified eagle′s medium (DMEM) to culture epithelial cell line and human endometrial cell line, minimum essential medium eagle to culture human breast adenocarcinoma cell line and medium 199 to culture normal ovarian cell line.

Insulin solution human is recommended for use in cell culture applications at 0.5 to 1mL per liter of medium.
Insulin solution human has been used for insulin tolerance test in mice and rats. It has also been used for adipogenic differentiation of human embryonic stem cells (hESCs)-derived outgrowth cultures and hMSCs (human mesenchymal stromal cells).
Usage
Recombinant human insulin solution is often added to the medium for cell culture, and the medium is diluted to the target concentration. It can also be diluted with sterile water or PBS. 1.According to the experimental requirements, the recombinant human insulin solution was diluted to the required working concentration. The common concentration of insulin in many cell cultures is 1-10 μg / mL, which is mainly determined by cell type. 2.Add the diluted solution to the medium according to the target concentration. For example, if you need to add 5 μg / mL insulin to 100 mL medium, 500 μL of 10 μg / mL insulin solution can be added.
Announcement
1.This product is filtered and sterilized by 0.22μm.When using this product, attention should be paid to aseptic operation to avoid pollution ; 
2.This product is 10 mg / mL concentrate, please use it after dilution as needed ; 
3.2-8 °C thawed, mixed well after use, avoid repeated freezing and thawing, less dosage is recommended subpackage cryopreservation, 2-8 °C preservation, at least 6 months effective ; 
4.It should not be placed at room temperature or higher temperature for a long time ; 
5.If there is precipitation after dissolution, the solution can be dissolved by mixing after the solution is raised to room temperature ; if it cannot be dissolved, use sterile 0.1 M HCl to slowly adjust the pH to between 2-3 ( while adjusting, while using the pH test paper to detect pH ) ; if still unable to dissolve, please contact us in time ; 
6.This product is only used for scientific research or further research, not for diagnosis and treatment.

Specifications

Product Name

Insulin solution human, CAS No.11061-68-0

Grade

BioReagent

Specifications & Purity

BioReagent, 10mg/ml

Biochemical and Physiological Mechanisms

Insulin plays a role in various cellular processes, such as promoting cell growth, regulating the cell cycle, and facilitating glucose uptake. It is commonly used as a protein supplement in serum-free mammalian cell culture media to help promote the optim

CAS

11061-68-0

Molecule Type

Unknown

Storage and Shipping

Concentration

10mg/ml

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Frequently Asked Questions

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 11061-68-0.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.