Recombinant Human DNTTIP1 Protein: Properties & Applications

Recombinant Human DNTTIP1 Protein is a highly purified recombinant form of the deoxynucleotidyl transferase terminal interacting protein 1, produced for use as a research reagent in studies of chromatin regulation, epigenetic gene silencing, and protein–protein interaction analyses.

Protein Name

Deoxynucleotidyl Transferase Terminal Interacting Protein 1 (DNTTIP1)

Expression System

Recombinant (specific host expression system to be confirmed; see product datasheet)

Species Origin

Human (Homo sapiens)

Primary Research Applications

Protein interaction studies, epigenetic research, chromatin complex characterization, biochemical assays

Storage Recommendation

Refer to product datasheet for recommended buffer, temperature, and aliquoting guidelines

SKU

rp368859

Biological Background of DNTTIP1

DNTTIP1, also known as Deoxynucleotidyl Transferase Terminal Interacting Protein 1, is a nuclear protein that was originally identified through its interaction with terminal deoxynucleotidyl transferase (TdT), an enzyme involved in V(D)J recombination during lymphocyte development. Beyond this initial characterization, DNTTIP1 has been found to function as a component of the Mitotic Deacetylase (MiDAC) complex, a multi-subunit histone deacetylase (HDAC) complex that plays a role in chromatin organization and transcriptional regulation.

The MiDAC complex, which includes DNTTIP1 alongside HDAC1/2, MIDEAS, and other associated factors, has been implicated in mitotic regulation and developmental gene expression programs. DNTTIP1 is thought to serve as a scaffolding or regulatory subunit that helps define substrate specificity and complex assembly. Its involvement in these fundamental epigenetic regulatory pathways makes it a protein of significant interest in oncology, developmental biology, and chromatin biology research.

Biochemical Properties and Product Specifications

Recombinant Human DNTTIP1 Protein (SKU: rp368859) is produced using recombinant protein expression technology to yield a product suitable for use in research applications requiring defined biochemical activity and high purity. As with all recombinant proteins from Aladdin Scientific, researchers should consult the official product datasheet for precise specifications, including molecular weight, purity grade (typically assessed by SDS-PAGE and/or HPLC), endotoxin levels, formulation buffer composition, and recommended storage conditions.

Key parameters to verify on receipt include: protein concentration (commonly determined by BCA or Bradford assay), purity percentage, and the presence of any tags (such as His-tag or GST-tag) that may influence downstream experimental design. Aliquoting upon first thaw is strongly recommended to avoid repeated freeze-thaw cycles that can compromise protein integrity and activity.

Research Applications

Recombinant Human DNTTIP1 Protein is suited to a range of biochemical and cell biology research applications, including but not limited to:

• Protein–Protein Interaction Studies: DNTTIP1's role as a component of the MiDAC HDAC complex makes it a valuable tool for pull-down assays, co-immunoprecipitation validation experiments, and surface plasmon resonance (SPR) or bio-layer interferometry (BLI) binding studies to map interactions with HDAC1/2, MIDEAS, and other binding partners.
• Epigenetic Research: As a constituent of an HDAC complex, recombinant DNTTIP1 can be employed in studies probing histone deacetylase complex assembly, regulation, and substrate recognition.
• Antibody Validation and Immunoassay Development: High-purity recombinant protein serves as a positive control antigen for ELISA development, western blot calibration, and antibody specificity validation.
• Structural Biology: Purified recombinant DNTTIP1 may support crystallography or cryo-EM studies aimed at resolving the architecture of the MiDAC complex or DNTTIP1-containing sub-complexes.
• Drug Discovery: In the context of HDAC complex biology, DNTTIP1 may serve as a component in biochemical screening assays for inhibitor development targeting epigenetic regulators.

Handling, Storage, and Quality Considerations

To maintain the integrity and activity of Recombinant Human DNTTIP1 Protein, the following general best practices are recommended, pending confirmation of specific conditions in the product datasheet:

• Storage Temperature: Recombinant proteins are typically stored at –80 °C for long-term preservation. Short-term storage at –20 °C may be acceptable depending on formulation.
• Freeze-Thaw Cycles: Minimize freeze-thaw cycles by preparing single-use aliquots upon first thaw. Each freeze-thaw cycle can result in protein aggregation, loss of activity, and degradation.
• Carrier Protein: Some formulations include bovine serum albumin (BSA) as a stabilizer; researchers should verify whether BSA is present if it may interfere with downstream assays.
• Handling: Work on ice when possible and avoid prolonged exposure to room temperature. Use low-protein-binding tubes to minimize adsorption losses.
• Quality Control: Aladdin Scientific's recombinant proteins undergo quality control testing. Review the certificate of analysis (CoA) provided with each lot for purity and activity data.

Ordering Information

Recombinant Human DNTTIP1 Protein is available from Aladdin Scientific under SKU rp368859. For current pricing, available pack sizes, lead times, and bulk or custom quotations, please contact the Aladdin Scientific sales team or visit the product page on the Aladdin Scientific website.

When placing an order, ensure that the desired quantity, required documentation (such as SDS or CoA), and any special shipping requirements are communicated at the time of order to facilitate timely delivery. Aladdin Scientific offers technical support for questions related to protein reconstitution, application optimization, and experimental design.

Frequently asked questions

What is DNTTIP1 and why is it relevant to epigenetics research?

DNTTIP1 (Deoxynucleotidyl Transferase Terminal Interacting Protein 1) is a nuclear protein and component of the MiDAC histone deacetylase complex, which regulates chromatin structure and gene expression. Its role in HDAC complex biology makes it directly relevant to studies of epigenetic gene regulation, mitotic control, and developmental biology.

What applications is Recombinant Human DNTTIP1 Protein suitable for?

Recombinant Human DNTTIP1 Protein is suitable for protein–protein interaction studies (e.g., pull-down, SPR, BLI), epigenetic complex characterization, antibody validation, ELISA development, western blot positive controls, and potentially structural biology and drug discovery applications targeting HDAC complexes.

How should Recombinant Human DNTTIP1 Protein be stored to maintain activity?

Generally, recombinant proteins should be stored at –80 °C for long-term storage and aliquoted upon first thaw to minimize freeze-thaw cycles. Always refer to the specific product datasheet and certificate of analysis supplied with your lot for the exact storage buffer, temperature, and stability information.

Does Recombinant Human DNTTIP1 Protein carry an affinity tag?

Tag information (e.g., His-tag, GST-tag, or tag-free) should be confirmed by consulting the official product datasheet for SKU rp368859. The presence or absence of an affinity tag is important for designing pull-down experiments and ensuring compatibility with downstream detection methods.

How can I request a bulk quotation or custom formulation for DNTTIP1 protein?

For bulk quantities, custom concentrations, or special formulations of Recombinant Human DNTTIP1 Protein (rp368859), please contact the Aladdin Scientific sales or technical support team directly via the website or by phone. Custom projects may have extended lead times.

References

1. Bantele SCS, Pfander B. Quantitative sensing and signalling of single-stranded DNA during the DNA damage response. Nucleic Acids Research. (General reference for nuclear protein complexes — flagged for review).
2. Itoh T, et al. Structural and functional characterization of a novel MiDAC histone deacetylase complex component. (Flagged: specific MiDAC complex composition and DNTTIP1 role — citation details not confirmed from source material).
3. Aladdin Scientific. Recombinant Human DNTTIP1 Protein, SKU rp368859. Product Datasheet. (Consult current datasheet at aladdinscientific.com for verified specifications).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.