Recombinant Human KLF17 Protein: Research Guide
Recombinant Human KLF17 Protein: Research Guide
Recombinant Human KLF17 Protein is a eukaryotic zinc finger transcription factor produced for use in research applications investigating gene regulation, tumor suppression, and epithelial-mesenchymal transition.
- Product Name
- Recombinant Human KLF17 Protein
- SKU
- rp364940
- Protein Family
- Krüppel-like Factor (KLF) family, zinc finger transcription factors
- Biological Function
- Transcriptional regulation; implicated in tumor suppression and inhibition of epithelial-mesenchymal transition
- Research Applications
- Gene regulation studies, cancer biology, EMT research, protein interaction assays
- Reference Number
- absea#PP-524
What Is KLF17? Biological Background
KLF17 (Krüppel-like Factor 17) is a member of the Krüppel-like factor (KLF) family of zinc finger transcription factors. Members of this family are characterized by three highly conserved C2H2-type zinc finger domains located at the C-terminus, which mediate sequence-specific binding to GC-rich and CACCC elements in target gene promoters.
KLF17 has attracted significant research interest for its role as a putative tumor suppressor. Studies in multiple cancer model systems have suggested that KLF17 expression is frequently downregulated in malignant tissues compared with normal counterparts. Mechanistically, KLF17 has been reported to suppress tumor metastasis in part by inhibiting epithelial-mesenchymal transition (EMT), a process by which epithelial cells acquire mesenchymal characteristics that promote invasiveness and dissemination. KLF17 is thought to achieve this in part through transcriptional repression of key EMT regulators.
Beyond oncology, KLF17 participates in broader transcriptional regulatory networks controlling cell differentiation and development, though many aspects of its biology remain under active investigation.
Protein Characteristics and Production
Recombinant Human KLF17 Protein (rp364940) is produced using recombinant expression systems to deliver a well-defined, research-grade protein suitable for in vitro biochemical and cell-based assays. The recombinant format ensures batch-to-batch consistency and removes the variability inherent in protein isolated directly from human tissues.
As a zinc finger protein, KLF17 requires proper folding of its C2H2 domains to maintain DNA-binding activity. Researchers should ensure that storage and handling conditions preserve protein integrity. The reagent is referenced under absea#PP-524 and is supplied by Aladdin Scientific as SKU rp364940.
Specific details on host expression system, purity percentage, molecular weight of the expressed construct, formulation buffer, and recommended storage temperature are subject to the product datasheet (absea#PP-524). Researchers are advised to consult the most current Certificate of Analysis (CoA) for lot-specific quality data.
Research Applications
Recombinant Human KLF17 Protein is suitable for a range of in vitro research applications, including but not limited to:
- Transcription factor binding assays: Use in electrophoretic mobility shift assays (EMSA) or surface plasmon resonance to characterize KLF17 binding to target DNA sequences.
- Protein–protein interaction studies: Co-immunoprecipitation, pull-down assays, or yeast two-hybrid validation experiments to map KLF17 interaction partners.
- Antibody validation: As a positive control antigen for anti-KLF17 antibody development or validation by Western blot or ELISA.
- Functional EMT studies: Reconstitution experiments in cell-free or cell-based systems to elucidate KLF17-dependent suppression of EMT regulators.
- Drug discovery screening: As a target protein in small-molecule or peptide screens aimed at modulating KLF17 transcriptional activity.
Researchers in oncology, developmental biology, and gene regulation will find this recombinant protein a valuable tool for mechanistic investigations.
Handling, Storage, and Quality Considerations
To maintain the activity and integrity of Recombinant Human KLF17 Protein, researchers should adhere to the following general best practices, pending confirmation from the product-specific datasheet:
- Storage: Store lyophilized or liquid protein preparations according to the instructions provided on the CoA. Avoid repeated freeze–thaw cycles, which can lead to protein aggregation and loss of activity.
- Reconstitution: If the protein is supplied in lyophilized form, reconstitute gently in the recommended buffer. Allow the vial to equilibrate to room temperature before opening to prevent condensation-related dilution.
- Working aliquots: Prepare single-use aliquots upon first thaw to minimize degradation over time.
- Zinc ion integrity: As a zinc finger protein, KLF17 may be sensitive to chelating agents such as EDTA. Avoid buffers or assay conditions containing strong metal chelators unless specifically required by the experimental design.
All lot-specific purity, activity, and endotoxin data are provided in the accompanying Certificate of Analysis. Contact Aladdin Scientific technical support for further guidance.
Ordering Information
Recombinant Human KLF17 Protein is available from Aladdin Scientific. Use the details below when placing your order:
| SKU | Product Name | Reference |
|---|---|---|
| rp364940 | Recombinant Human KLF17 Protein | absea#PP-524 |
For bulk quantities, custom formulations, or additional technical information, please contact Aladdin Scientific customer support or visit the product page on the Aladdin Scientific website.
Frequently asked questions
What is the biological role of KLF17 in human cells?
KLF17 is a zinc finger transcription factor of the Krüppel-like factor family that binds GC-rich promoter elements to regulate target gene transcription. It has been implicated as a tumor suppressor, with reported roles in inhibiting epithelial-mesenchymal transition (EMT) and suppressing tumor cell invasiveness and metastasis. Its broader roles in development and differentiation remain an active area of research.
What applications is Recombinant Human KLF17 Protein (rp364940) suitable for?
This recombinant protein is suitable for in vitro applications including EMSA and DNA-binding assays, protein–protein interaction studies (pull-down, co-IP), antibody validation as a positive control antigen, functional reconstitution experiments related to EMT, and small-molecule screening assays. Refer to the product datasheet (absea#PP-524) for validated application details.
How should Recombinant Human KLF17 Protein be stored to maintain its activity?
General best practices include storing the protein according to the CoA instructions, avoiding repeated freeze–thaw cycles by preparing single-use aliquots, and keeping the protein away from chelating agents such as EDTA that may disrupt the zinc finger domains. Always consult the lot-specific Certificate of Analysis provided with your shipment for definitive storage conditions.
Why is KLF17 considered a tumor suppressor?
KLF17 is considered a putative tumor suppressor because its expression is frequently reduced or lost in various cancers relative to normal tissues, and experimental evidence suggests it can inhibit tumor cell migration, invasion, and metastasis by repressing EMT-related gene programs. However, the precise mechanisms and the extent of its role across different cancer types continue to be investigated.
Is Recombinant Human KLF17 Protein suitable for use as an antibody validation control?
Yes, recombinant proteins are commonly used as positive control antigens in Western blot and ELISA-based antibody validation experiments. Recombinant Human KLF17 Protein (rp364940) can serve this purpose, providing a defined source of KLF17 antigen. Confirm suitability for your specific antibody clone and assay format with the product datasheet or Aladdin Scientific technical support.
References
- Limame R, et al. Comparative analysis of dynamic cell viability, migration and invasion assessments by novel real-time technology and classic endpoint assays. PLoS One. 2012. [General KLF/EMT background — verify applicability to KLF17 specifically]
- Ghaleb AM, Yang VW. Krüppel-like factors (KLFs): components of a regulatory network controlling key aspects of cell biology. J Cell Physiol. 2017;232(8):1848-1857.
- Zhao W, et al. KLF17 inhibits epithelial-mesenchymal transition through the downregulation of Id1 in breast cancer cells. Oncol Rep. 2015. [Verify authors, journal, volume, pages, and PMID before publication]
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.