L-ANAP TFA - BioReagent, for Fluorescence analysis, for Microscopy, ≥98%(HPLC) , CAS No.2287346-69-2
In stock
SKU
L1452960
L-ANAP (ANAP) is a bright fluorescent amino acid that can be site-specifically incorporated into proteins using amber stop codon suppression.
Application
L-ANAP may be used to fluorescently label proteins and study its localization and dynamics within live mammalian cells.
Biochem/physiol Actions
L-ANAP (ANAP), a fluorescent structural probe, enables real-time monitoring of ion channel dynamics through site-specific incorporation and environmental sensitivity. ANAP features optimal spectral properties with λex ~ 360 nm and environment-dependent λem ~ 490 nm, Φ of 0.22–0.5, and an ϵ of 17.5 mM⁻¹ cm⁻¹ in water. Due to its environmental sensitivity and ability to serve as a FRET donor, ANAP enables simultaneous monitoring of protein conformational changes, ligand binding, protein-protein interactions, and temperature sensitivity while maintaining channel function. ANAP’s versatility is demonstrated through successful applications in various cell types (Xenopus oocytes, HEK-293, CHO, tSA-201 cells), where it can be combined with electrical recordings to provide direct correlation between structural states and channel function. ANAP’s small size, environment-sensitive fluorescence, and the capability to introduce it at specific sites in the proteome through simple mutagenesis make it a unique and valuable tool for eukaryotic cell biology.
More Information
| CAS | 2287346-69-2 |
|---|---|
| Specifications & Purity | BioReagent, for Fluorescence analysis, for Microscopy, ≥98%(HPLC) |
| Stability And Storage | Store at 2-8℃ long term (12 months). Store in the dark. Desiccated. |
| Storage | Store at 2-8°C,Protected from light,Desiccated |
| Shipped In | Wet ice |
| Product Description | L-ANAP (ANAP) is a bright fluorescent amino acid that can be site-specifically incorporated into proteins using amber stop codon suppression. Application L-ANAP may be used to fluorescently label proteins and study its localization and dynamics within live mammalian cells. Biochem/physiol Actions L-ANAP (ANAP), a fluorescent structural probe, enables real-time monitoring of ion channel dynamics through site-specific incorporation and environmental sensitivity. ANAP features optimal spectral properties with λex ~ 360 nm and environment-dependent λem ~ 490 nm, Φ of 0.22–0.5, and an ϵ of 17.5 mM⁻¹ cm⁻¹ in water. Due to its environmental sensitivity and ability to serve as a FRET donor, ANAP enables simultaneous monitoring of protein conformational changes, ligand binding, protein-protein interactions, and temperature sensitivity while maintaining channel function. ANAP’s versatility is demonstrated through successful applications in various cell types (Xenopus oocytes, HEK-293, CHO, tSA-201 cells), where it can be combined with electrical recordings to provide direct correlation between structural states and channel function. ANAP’s small size, environment-sensitive fluorescence, and the capability to introduce it at specific sites in the proteome through simple mutagenesis make it a unique and valuable tool for eukaryotic cell biology. |
| Solubility | Soluble in DMSO. |
| Sensitivity | Hygroscopic |
| Molecular Formula | C17H17F3N2O5 |
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