GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Suitable for mass spectrometry (MS)?MS grade — low non-volatile and ionic residues compatible with mass spectrometry. Use to minimize background and ion suppression in MS workflows.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥99%(HPLC)≥25 U/mg protein
EnzymoPure™,suitable for mass spectrometry (MS),Recombinant,≥99%(HPLC),≥25 U/mg protein Recombinant,Suitable for mass spectrometry (MS),EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
MS-grade Lys-C protease specifically cleaves peptide bonds at the C-terminus of lysine residues. It can improve the digestion of hard-to-hydrolyze proteins and is recommended for use in 6–8 M urea solutions.
Reconstitution Solution:
Reconstitute Lys-C protease in 50 mM acetic acid buffer.
pH Range:
rLys-C exhibits optimal activity at pH 8–9.
Application:
Lys-C protease specifically cleaves lysine (K) residues in denatured proteins that are resistant to proteolysis. It is recommended for use in combination with trypsin.
Recommended SOP for Mixed Protease Digestion:
A. General In-Solution Digestion
Use a mixture of 100 μg trypsin and 20 μg Lys-C at a protein-to-protease ratio (w/w) of 25:1. Incubate overnight at 37°C for 13–16 hours.
Tip: Urea concentration should be <1 M; use 50 mM Tris-HCl buffer (pH 8).
B. 1+1 Step In-Solution Digestion
1. Use a mixture of 100 μg trypsin and 20 μg Lys-C at a protein-to-protease ratio (w/w) of 25:1. Incubate at 37°C for 2–4 hours.
Tip: Urea concentration should be 6–8 M; use 50 mM Tris-HCl buffer (pH 8).
2. Dilute the solution 6-fold and incubate overnight at 37°C for 13–16 hours.
Tip: Urea concentration should be <1 M; use 50 mM Tris-HCl buffer (pH 8).
Specifications
Product Name
Lys-C (MS), CAS No.72561-05-8
Grade
Recombinant, Suitable for mass spectrometry (MS), EnzymoPure™
Specifications & Purity
EnzymoPure™,suitable for mass spectrometry (MS),Recombinant,≥99%(HPLC),≥25 U/mg protein
CAS
72561-05-8
Enzyme Commission Number
EC 3.4.21.50
Molecule Type
Enzyme
Storage and Shipping
Concentration
≥25 U/mg protein
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Unit definition
The amount of rLys C that hydrolyzes 1.0 μmol of T-G-P Lys Nitroanilid per minute at 405 nm, under conditions of pH 7.8 and 25°C.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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