Methyl Green - zinc chloride salt, ~65% , CAS No.7114-03-6

CAS: 7114-03-6 Cat. No.: M100214 Molecular Weight: 516.4（free base） Beilstein Registry Number: 3914106 EC Number: 230-415-4 PubChem CID: 16211442

GRADE & PURITY zinc chloride salt, ~65%

Synonyms

C.I.42590 | Methyl Green zinc chloride salt (C.I. 42590) | Ethyl Green, zinc chloride salt | Methyl Green zinc chloride salt | 4-((4-(dimethylamino)phenyl)(4-(dimethyliminio)cyclohexa-2,5-dienylidene)methyl)-N-ethyl-N,N-dimethylbenzenaminium zinc(II) brom

Storage

Room temperature

Shipped In

Normal

Overview

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Specifications

Synonyms

C.I.42590 | Methyl Green zinc chloride salt (C.I. 42590) | Ethyl Green, zinc chloride salt | Methyl Green zinc chloride salt | 4-((4-(dimethylamino)phenyl)(4-(dimethyliminio)cyclohexa-2, 5-dienylidene)methyl)-N-ethyl-N, N-dimethylbenzenaminium zinc(II) brom

Specifications & Purity

zinc chloride salt, ~65%

Biochemical and Physiological Mechanisms

DNA stain that binds selectively to AT-rich regions of native DNA. Used as a tracking dye in acid buffers for electrophoresis. Also used in Methyl Green-Pyronin methods to demonstrate DNA and RNA in tissue sections.

Storage

Room temperature

Shipped In

Normal

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Names and Identifiers

• Pubchem Sid: 504768105
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504768105
• Canonical Smiles: CC[N+](C)(C)C1=CC=C(C=C1)C(=C2C=CC(=[N+](C)C)C=C2)C3=CC=C(C=C3)N(C)C.[Cl-].Cl[Zn]Cl.[Br-]
• IUPAC Name: dichlorozinc;[4-[[4-(dimethylamino)phenyl]-(4-dimethylazaniumylidenecyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-ethyl-dimethylazanium;bromide;chloride
• InChIKey: RRKSQWOQZFIQKW-UHFFFAOYSA-J
• INCHI: 1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
• Isomeric SMILES: CC[N+](C)(C)C1=CC=C(C=C1)C(=C2C=CC(=[N+](C)C)C=C2)C3=CC=C(C=C3)N(C)C.[Cl-].Cl[Zn]Cl.[Br-]
• WGK Germany: 3
• PubChem CID: 16211442
• Molecular Weight: 516.4（free base）
• Beilstein: 3914106

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Benzenoids
    • Class Benzene and substituted derivatives
      • Subclass Diphenylmethanes

• Kingdom: Organic compounds
• Superclass: Benzenoids
• Class: Benzene and substituted derivatives
• Subclass: Diphenylmethanes
• Intermediate Tree Nodes: Not available
• Direct Parent: Diphenylmethanes
• Alternative Parents: Dialkylarylamines Aniline and substituted anilines Secondary ketimines Quaternary ammonium salts Azomethines Propargyl-type 1,3-dipolar organic compounds Organic transition metal salts Organic metal bromide salts Organopnictogen compounds Organic chloride salts Organic bromide salts Hydrocarbon derivatives
• Molecular Framework: Aromatic homomonocyclic compounds
• Substituents: Diphenylmethane - Aniline or substituted anilines - Dialkylarylamine - Tertiary aliphatic/aromatic amine - Secondary ketimine - Quaternary ammonium salt - Azomethine - Tertiary amine - Organic metal bromide salt - Organic metal salt - Organic transition metal salt - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Organic nitrogen compound - Organopnictogen compound - Hydrocarbon derivative - Organic chloride salt - Organic bromide salt - Organic salt - Organonitrogen compound - Amine - Aromatic homomonocyclic compound
• Description: This compound belongs to the class of organic compounds known as diphenylmethanes. These are compounds containing a diphenylmethane moiety, which consists of a methane wherein two hydrogen atoms are replaced by two phenyl groups.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Soluble in water, ethanol or methanol.
• Sensitivity: Light sensitive
• Melt Point(°C): >300°C
• Molecular Weight: 653.200 g/mol
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 5
• Exact Mass: 649.037 Da
• Monoisotopic Mass: 649.037 Da
• Topological Polar Surface Area: 6.300 Ų
• Heavy Atom Count: 35
• Formal Charge: 0
• Complexity: 648.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 4

XLogP3

References

1. Abdul Ghaffar, Muhammad Hassan, Oleksiy V. Penkov, Cafer T. Yavuz, Kemal Celebi.  (2023)  Tunable Molecular Sieving by Hierarchically Assembled Porous Organic Cage Membranes with Solvent-Responsive Switchable Pores.  ENVIRONMENTAL SCIENCE & TECHNOLOGY,      [PMID:37965815] [10.1021/acs.est.3c05883]

2. Mei-Ling Liu, Yu-Xuan Sun, Ying Tang, Zheng-Jun Fu, Qian Wang, Zhen-Yuan Wang, Shi-Peng Sun.  (2023)  Graphene oxide hollow fiber membranes for solvent dehydration by nanofiltration.  JOURNAL OF MEMBRANE SCIENCE,      [PMID:] [10.1016/j.memsci.2023.121848]

3. Zhang Na, Li Yang, Feng Zeguo.  (2023)  Inhibition effect of choline and parecoxib sodium on chronic constriction nerve injury-induced neuropathic pain in rats.  BMC Anesthesiology,  23  (1): (1-9).  [PMID:36639747] [10.1186/s12871-022-01913-0]

4. Kai Niu, Pengcheng Sun, Jiping Chen, Xianbo Lu.  (2022)  Dense Conductive Metal–Organic Frameworks as Robust Electrocatalysts for Biosensing.  ANALYTICAL CHEMISTRY,      [PMID:36454682] [10.1021/acs.analchem.2c03766]

5. Yang Cao, Jianquan Luo, Chulong Chen, Yinhua Wan.  (2021)  Highly permeable acid-resistant nanofiltration membrane based on a novel sulfonamide aqueous monomer for efficient acidic wastewater treatment.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2021.131791]

6. Yue Zhang, Kongyin Zhao, Zhijian Zhang, Huike Xie, Ziyi Li, Ze Lin, Chen Mo, Wendong Yang, Xiaolei Wang, Junfu Wei.  (2020)  Polypropylene non-woven supported calcium alginate hydrogel filtration membrane for efficient separation of dye/salt at low salt concentration.  DESALINATION,      [PMID:] [10.1016/j.desal.2020.114845]

7. Man-Tong Zhao, Zhong-Yuan Liu, Ao Li, Guan-Hua Zhao, Hong-Kai Xie, Da-Yong Zhou, Tong Wang.  (2020)  Gallic acid and its alkyl esters emerge as effective antioxidants against lipid oxidation during hot air drying process of Ostrea talienwhanensis.  LWT-FOOD SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.lwt.2020.110551]

8. Yi Yang, Jing Meng, Haoquan Li, Dongyu Gu, Shuai Wang, Shuai He, Haiyu Xu, Yoichiro Ito.  (2020)  Elution-extrusion and back-extrusion counter-current chromatography using three-phase solvent system for separation of organic dye mixture.  SEPARATION AND PURIFICATION TECHNOLOGY,      [PMID:] [10.1016/j.seppur.2020.117024]

9. Linlin Xu, Hua Zhang, Yue Tian, Anxin Jiao, Shuang Li, Yang Tan, Ming Chen, Feng Chen.  (2019)  Modified photochemical strategy to support highly-purity, dense and monodisperse Au nanospheres on graphene oxide for optimizing SERS detection.  TALANTA,      [PMID:31892060] [10.1016/j.talanta.2019.120535]

10. Xiao Wang, Xiaohui Ju, Tian-Zhi Jia, Qian-Cheng Xia, Jia-Lin Guo, Chen Wang, Zhaoliang Cui, Yong Wang, Weihong Xing, Shi-Peng Sun.  (2018)  New surface cross-linking method to fabricate positively charged nanofiltration membranes for dye removal.  JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,  93  (8): (2281-2291).  [PMID:] [10.1002/jctb.5571]

11. Qing Tian, Haoyu Wang, Shuaiqi Huangfu, Rong Yang, Yao Chen, Jie Gao, Yingchun Yang, Lianbing Zhang.  (2025)  Oxygen Vacancy Formation Energy Determines the Phase-Activity Relationship of MnO2 Laccase Nanozymes.  ACS Applied Materials & Interfaces,      [PMID:40129185] [10.1021/acsami.4c22599]

12. Cheng-Ye Zhu, Jia-Hui Xin, Chao Zhang, Hao-Cheng Yang, Hong-Qing Liang, Zhi-Kang Xu.  (2025)  Designing polyurea membranes with twisted and durable skeleton for thermal/acid-stable efficient dye/salt separation.  JOURNAL OF MEMBRANE SCIENCE,      [PMID:] [10.1016/j.memsci.2025.124770]