GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.≥95%(SDS-PAGE)0.2 mg/mL
Recombinant, Azide Free, Validated, High Performance, Animal Free, ExactAb™, ≥95%(SDS-PAGE), 0.2 mg/mL Animal Free,Azide Free,ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Non-specific binding to Leukocytes is a well-known challenge in flow cytometry. Though the specific mechanism of non-specific binding is not known, the binding of cyanine acceptor fluorophores to CD64 has been implicated in the literature. Mouse FcR Blocking Reagent blocks non-antigen-specific binding of immunoglobulins to the FcγIII and FcγII, and possibly FcγI, Receptors in vitro and in vivo. Furthemore, it effectively inhibits non-specific staining by various PE, PerCP, and APC tandem dyes on leukocytes. Mouse FcR Blocking Reagent blocks both clone P90A23 and 2.4G2 raised against mouse CD16/32.
Specifications
Product Name
Mouse FcR Blocking Reagent - Primary antibody, specific to Mouse CD16/CD32
Synonyms
Fc block | Fc Receptor Blocker | FcR Blocking Reagent, mouse | Mouse Fc blocker | Fc receptor blocking antibody | Mouse Fc Receptor Blocking Solution | Fc Receptor Binding Inhibitor Antibody | anti-Mouse Fc Block Monoclonal Antibody | Fc Receptor Blocking
CD16: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b.
Mediates neutrophil activation by IgG complexes redundantly with Fcgr4.
CD32: Receptor for the Fc region of complexed immunogl
Host species
Rat
Specificity
Mouse CD16/CD32
Immunogen
Mouse BALB/c Macrophage J774
Positive Control
Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation
Unconjugated
Grade
Animal Free, Azide Free, ExactAb™, High Performance, Recombinant, Validated
Light Chain Type
lambda
Purification Method
Protein A purified
Concentration
0.2 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
Store at 4°C.
Images
Mouse FcR Blocking Reagent (Ab219990) - Flow Cytometry Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with Mouse FcR Blocking Reagent. C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) after being blocked with Mouse FcR Blocking Reagent (Ab219990) (right panel) compared to an no blocking control (left panel). Left panel - No blocking control, cells were stained with Rat IgG (PE) (Ab180312) without any blocking reagent.
Mouse FcR Blocking Reagent (Ab219990) - Flow Cytometry Blocking of non-specific Fc Receptor-mediated fluorescent antibody binding with Mouse FcR Blocking Reagent. C57BL/6 splenocytes were stained with Rat IgG (PE) (Ab180312) after being blocked with Mouse FcR Blocking Reagent (Ab219990) (red) or 5% mouse serum (green). Blue - No blocking control, cells were stained with Rat IgG (PE) (Ab180312) without any blocking reagent; Black - Unlabelled control, cells without incubation with any antibody.
Application
Application
Dilution info
Flow Cytometry
5.0 μL/test. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with Mouse FcR blocking reagent as 5.0 μL per 106 cells in 100 µl volume for 30 minutes at 4℃ prior to immunostai
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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