MTT Solution [for Cell proliferation assay] - 5.0 mg / mL in PBS , CAS No.M405849

CAS: M405849 Cat. No.: M405849

GRADE & PURITY 5.0 mg / mL in PBS

Synonyms

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2|H|-tetrazol-3-ium Bromide Solution

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Product description：

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide (MTT) is a type of tetrazolium salt that changes to formazan when taken up into living cells. As formazan is a non-water soluble crystal, it can be dissolved in DMSO and the absorbance at 570 nm can be used to count the number of cells. This product consists of 5.0 mg / mL MTT dissolved in PBS. When 1/10th of the medium is added to cultured cells, the live cells are properly stained.

Product Information：

Volume/Quantity : 1mL x 5

Product Form : 5.0 mg / mL in PBS

Directions for Use：

MTT solution can be added at any point in the cell culture. For best results, it should be added to cells in the logarithmic growth phase. Pre-determine the number of cells and seed 10,000 to 100,000 cells per well in a 96-well plate.

1. Seed 100 μL of cells in a 96-well plate.

2. Incubate overnight in an incubator.

3. Add 10 μL of MTT solution to each well.

4. Incubate for 2-4 hours for color reaction.

5. The medium is removed, taking care not to inhale the formazan.

6. Add 100 μL of DMSO (Product No. D0798) and dissolve the formazan.

7. Measure the absorbance at 570 nm.

Storage：

This product should be stored at -20 °C and should be protected from light. Avoid repeated freezing and thawing.

Specifications

Synonyms

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2 | H | -tetrazol-3-ium Bromide Solution

Specifications & Purity

5.0 mg / mL in PBS

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Chemical and Physical Properties

• Sensitivity: Light & Heat Sensitive

References

1. Jiayi Zhou, Sai Zhang, Yu Zhang, Tianliang Liu, Shuying Yang, Gaopeng Lv, Yaping Wang, Kewei Feng, Yahong Yuan, Tianli Yue, Qinglin Sheng.  (2024)  Silver nanoparticle-functionalized covalent organic frameworks for the inhibition of foodborne pathogenic bacteria and their application in green grape preservation.  FOOD CHEMISTRY,      [PMID:39303470] [10.1016/j.foodchem.2024.141310]

Frequently Asked Questions

How should this product be stored?

Store at ?20 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is M405849.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.