Mouse anti Human NIT2 Antibody, Monoclonal (OTI3D5), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.
NIT2 Mouse mAb (Ab118115) - Western Blot
All lanes: NIT2 Mouse mAb (Ab118115) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 20.9 s
NIT2 Mouse mAb (Ab118115) - Western Blot
Equivalent amounts of cell lysates (10 µg per lane) of wild-type 293T cells (WT) and NIT2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with NIT2 Mouse mAb (Ab118115), (1/500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.
NIT2 Mouse mAb (Ab118115) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of colon tissue using NIT2 Mouse mAb (Ab118115). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10min, Dilution 1/50).
NIT2 Mouse mAb (Ab118115) - IHC
Immunohistochemical staining of paraffinembedded Kidney tissue within the normal limits using NIT2 Mouse mAb (Ab118115). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
NIT2 Mouse mAb (Ab118115) - IHC
Immunohistochemical staining of paraffinembedded liver tissue within the normal limits using NIT2 Mouse mAb (Ab118115). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
NIT2 Mouse mAb (Ab118115) - IF/ICC
NIT2 Mouse mAb (Ab118115) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2.
NIT2 Mouse mAb (
Ab118115
) - Flow Cytometry
HEK293T cells transfected with either pCMV6-ENTRY NIT2 (Red) or empty vector control plasmid (Blue) were immunostained with NIT2 Mouse mAb (Ab118115), and then analyzed by flow cytometry.
1/1000
1/100
1/100
1/50
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ25F0117256 | Certificate of Analysis | Aug 04, 2026 | Ab118115 |
| ZJ25F0117257 | Certificate of Analysis | Aug 04, 2026 | Ab118115 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Validated grade guide → View KO Validation grade guide → View ExactAb™ grade guide →
