Pimicotinib hydrochloride , CAS No.2866305-19-1

CAS: 2866305-19-1 Cat. No.: P1450614 Formula: C₂₂H₂₅ClN₆O₃ Molecular Weight: 456.93

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Pimicotinib hydrochloride is a CSF1R inhibitor with antitumor activity.

Specifications

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Action Type

INHIBITOR

Names and Identifiers

• Molecular Weight: 456.93

Application Protocols

No tested application protocols are provided for this item.

General guidance for in vitro use (not item-specific):

• Stock preparation: Dissolve in anhydrous DMSO to a convenient concentration (e.g., 10 mM). Vortex and sonicate briefly if needed. Record exact mass and volume for traceability.
• Working solutions: Dilute into pre-equilibrated assay buffer or culture medium immediately before use. Maintain consistent final DMSO content across control and treated samples.
• Controls: Include vehicle controls and, where appropriate, orthogonal positive/negative controls to benchmark assay performance.
• Storage of stocks: Aliquot to avoid repeated freeze–thaw. Protect from moisture; minimize headspace.

For analytical methods:

• Prepare calibration curves across the intended quantitation range using freshly prepared standards.
• Assess short-term and long-term stability at relevant temperatures (bench-top, autosampler, refrigerated, and frozen) per internal SOPs.

All protocols should be validated under your laboratory’s QA system. Refer to the product’s CoA and SDS for any item-specific constraints.

Biological Roles

No biological mechanism or target information is provided in the Product Data. The following notes are general literature context and must not be construed as product specifications or recommendations.

General/literature context (not item-specific):

• Pimicotinib has been described in the research literature as a small-molecule tool compound developed to modulate signaling mediated by receptor tyrosine kinases in the colony-stimulating factor 1 receptor (CSF1R) pathway. Such agents are commonly investigated to study macrophage biology, microglial function, and downstream immunoregulatory signaling cascades in preclinical systems.
• As a hydrochloride salt, the compound is often selected for in vitro use where aqueous compatibility and consistent protonation state can be advantageous.

Laboratory study considerations:

• When used as a research probe, define the assay window, verify target engagement using orthogonal readouts (e.g., biochemical kinase assay and cellular phospho-protein measurements), and include counterscreens for off-target families as appropriate.
• Establish stability and protein-binding characteristics in your assay matrix; adsorption to plastics and nonspecific binding to serum proteins can influence apparent potency.

Important limitations:

• No claims are made here regarding selectivity, potency, or kinetics for this specific item. Consult primary literature and generate internal controls under your assay conditions to confirm activity profiles.

Buffer Applications

This product is a small-molecule research compound rather than a buffering agent. Consequently, it is not used to set or maintain pH in typical laboratory buffers.

Practical notes (general):

• If diluting concentrated DMSO stocks into buffers, select a buffer system compatible with your assay (e.g., phosphate, HEPES, or acetate) and ensure the final DMSO content is within assay tolerance (commonly ≤0.5–1% v/v).
• For hydrochloride salts, mildly acidic buffers (pH 4–6) can help maintain solubility of protonated amines. However, optimal pH must be determined experimentally for your specific assay.

If you require a buffering agent, refer to dedicated buffer products (e.g., HEPES, MOPS, PBS components) in the catalog.

Green Alternatives

This product is a specific small-molecule research compound, not a commodity solvent or reagent with interchangeable roles. Therefore, the concept of greener substitutes applies primarily to the choice of dissolution and processing solvents rather than to replacement of the compound itself.

Greener handling choices (general, not item-specific):

• Prefer water or aqueous buffers where feasible for working solutions, provided the hydrochloride salt maintains solubility and stability.
• If an organic cosolvent is needed, consider lower-hazard options first (e.g., ethanol) and minimize volumes of high-toxicity aprotic solvents (DMSO, DMF, NMP) consistent with assay requirements.
• Use micro-scale preparations to reduce chemical inventory and waste; adopt closed-vial dissolution and low-headspace containers to limit volatilization and exposure.

Comparison of common cosolvents (general):

• Water/buffer: Highest safety and sustainability; may require acidic pH to maintain solubility for amine salts.
• Ethanol: Renewable source potential; good compatibility with many biological assays at low % v/v.
• DMSO: Excellent solubilizer; persistent in the environment; use minimal necessary concentrations.
• DMF/NMP: Strong solubilizers but reproductive toxins; avoid if alternatives meet performance needs.

Waste minimization:

• Combine small-volume residues and dispose via approved aqueous or organic waste streams. Validate LC–MS mobile phases that reduce acetonitrile consumption when possible (e.g., higher aqueous content if chromatographically acceptable).

Pharmaceutical Uses

No pharmacopeial status, excipient role, or formulation specification is provided for this item. It is supplied strictly for research use only, as stated in the Product Data.

Permissible research-oriented uses (general):

• Analytical reference standard: Method development and validation in discovery or preclinical analytical labs (e.g., LC–MS/MS quantitation, stability-indicating assays).
• In vitro tool compound: Investigations of biochemical pathways in cell-free or cell-based systems.

Non-clinical disclaimer:

• Not for human or veterinary use. Not for diagnostic procedures. Do not incorporate into products intended for therapeutic administration.

Formulation notes for research settings (not item-specific):

• Stock solutions are commonly prepared in DMSO due to solubility and stability considerations, then diluted into aqueous media for experiments.
• If preparing solid-dose surrogates for device testing or dissolution studies, document excipients and processing parameters; these activities remain non-clinical and should not imply suitability for administration.

Physical Properties

Item-specific physical properties:

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point, boiling point, density, refractive index, UV cutoff, water content, residual solvents, elemental analysis: Not specified for this item; refer to CoA/Spec Sheet.

General/literature guidance for hydrochloride salts of basic small molecules (not item-specific):

• Physical state: Often crystalline solids or amorphous powders; salts commonly exhibit higher melting points than corresponding free bases.
• Solubility profile: Hydrochloride salts typically show improved solubility in polar protic solvents (water, methanol, ethanol) and in dipolar aprotic solvents (DMSO, DMF). Actual solubility should be measured for this lot.
• pKa considerations: Protonated amines (as HCl salts) are fully ionized in neutral water; the free base pKa governs the degree of ionization upon basification. Specific pKa values for pimicotinib are not provided here.
• Hygroscopicity: Some amine hydrochlorides can be mildly hygroscopic; monitor mass and seal promptly after use if applicable.

Important note:

• In the absence of product-specific measurements, do not assume exact solubility limits, melting point, or hydrate content. For quantitative work, determine solubility and verify identity via NMR/LC–MS using the provided CoA as primary reference.

Quality & Grades

Item-specific quality details:

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.

How to interpret typical quality descriptors (general guidance):

• Purity (e.g., ≥98% by HPLC): Indicates chromatographic assay of the main component; impurities may include related substances, residual solvents, or inorganic salts. Verify against the supplied CoA for this lot.
• Identity testing: LC–MS and 1H/13C NMR are commonly provided or recommended for confirmation. Salt content (chloride assay) may be reported for hydrochloride forms.
• Water/volatile content: Karl Fischer and loss-on-drying results, if provided, indicate hydration/solvent levels. Absent values here should be obtained from the CoA.
• Stabilizers: Not specified for this item; refer to CoA/Spec Sheet. If no stabilizer is used, store as instructed and protect from moisture/light as appropriate.

What is specific to this listing:

• Supplied for research use only (per Research Use Note).
• Cold-chain logistics (ice packs) and frozen storage (−20 °C) to maintain integrity.

Recommendations for QC on receipt (practical):

• Record lot number and verify integrity of packaging.
• Equilibrate to room temperature in sealed container before opening to minimize condensation.
• If quantitative work is planned, perform a quick check by LC–MS and HPLC purity against the CoA acceptance criteria.

Reaction & Applications

Manufacturer application text: Not provided for this item.

Use context (research-only): Pimicotinib hydrochloride is best regarded as a small-molecule research tool or analytical reference standard rather than as a synthetic reagent. As such, its “applications” center on biochemical/biophysical assay development, target engagement studies, and method validation.

Practical laboratory applications (general, not item-specific):

• Reference material: Use as a system-suitability or calibration compound for LC–UV/LC–MS in discovery workflows where pimicotinib is under study. Establish retention time windows and ion transitions for MRM.
• In vitro assay development: Prepare DMSO stocks and evaluate concentration–response relationships in enzyme or binding assays relevant to the research target. Maintain rigorous control of DMSO content and adsorption losses (use low-bind plastics or glass vials).
• Stability checks: Conduct bench-top, freeze–thaw, and photostability assessments under your laboratory conditions before long studies.

Not typically recommended as a reagent: Given its specialized structure and cost, pimicotinib hydrochloride is not generally used as a coupling partner, catalyst, or protecting group reagent. If synthetic derivatization is intended (e.g., to make probe analogs), confirm compatibility of functional groups and salt form before planning transformations.

Reaction Conditions

This product is not generally used as a reagent or substrate in standard organic transformations; therefore, no canonical “reaction conditions” apply.

If conducting solution preparations for assays (general, not item-specific):

• Solvent: DMSO for concentrated stocks; water or buffer for working solutions if solubility permits.
• Concentration ranges: Commonly 1–50 mM in DMSO for stocks; dilute to nM–µM in assays, controlling final DMSO content.
• Temperature: Dissolve at ambient temperature; gentle warming (25–37 °C) and brief sonication can aid dissolution. Avoid excessive heat or prolonged exposure to light unless stability is verified.
• Filtration: If particulates are observed, filter through 0.22 µm PTFE or PES (verify compatibility). Avoid adsorption losses by conditioning filters with solvent.

For any chemical transformations involving this scaffold (advanced users):

• Consult the exact structure from the CoA/SDS and survey literature for analogous heteroaromatic amine salts. Reaction media, bases, and protecting group strategies must be tailored to the functional groups present. No item-specific conditions are provided here.

Safety & Handling

Authoritative safety data: Always consult the SDS for this specific SKU (P1450614).

Item-specific hazard fields provided:

• Signal Word: Not specified for this item; refer to SDS.
• H-Statements: Not specified for this item; refer to SDS.
• GHS Classification/Pictograms: Not specified for this item; refer to SDS.

General laboratory precautions for small-molecule hydrochloride salts (not item-specific):

• PPE: Use lab coat, safety glasses, and suitable chemical-resistant gloves (e.g., nitrile). Handle powders in a fume hood to minimize inhalation of dust/aerosols.
• Avoid: Inhalation, ingestion, skin/eye contact. Prevent dust formation; use antistatic measures if handling fine powders.
• First aid (overview): In case of eye or skin contact, rinse with water for ≥15 minutes. If inhaled, move to fresh air; seek medical attention if symptoms persist. If ingested, rinse mouth; obtain medical advice.
• Incompatibilities: Strong bases may liberate the free base from the hydrochloride salt; strong oxidizers should be avoided unless process-relevant. Avoid moisture exposure if the material is hygroscopic.
• Thermal stability: Store as directed to prevent degradation.

Storage and transport (item-specific):

• Storage Conditions: Store at −20 °C.
• Shipped In: Ice chest + ice pads.

Waste: Dispose in accordance with institutional and local regulations; classify as organic laboratory chemical waste unless otherwise specified by SDS.

Solvent Selection

Item-specific solubility data are not provided; consult the CoA for definitive guidance.

General guidance for dissolving small-molecule hydrochloride salts for research use (not item-specific):

• Primary stock solvents: DMSO is typically preferred for small organic research compounds due to wide solubilizing power and compatibility with bioassays. DMF can be used when DMSO is insufficient, but consider toxicity and handling.
• Aqueous media: Many hydrochloride salts exhibit improved water solubility versus the free base. If preparing aqueous stocks, begin with water or buffered saline at pH 2–6 to maintain protonation; adjust pH cautiously if required.
• Alcohols: Methanol or ethanol can be used for intermediate concentrates; ensure compatibility with downstream assays.
• Miscibility considerations: DMSO and DMF are miscible with water and most organics, facilitating serial dilutions into buffers. Pre-warm gently (e.g., 25–37 °C) and sonicate to assist dissolution, avoiding prolonged heating.

Selection tips:

• For biochemical assays: Prepare a concentrated DMSO stock (e.g., 10–50 mM) and dilute into assay buffer, keeping final DMSO ≤0.5–1% v/v unless the assay tolerates more.
• For analytical reference standards: Use acetonitrile/water with 0.1% formic acid for LC–MS-compatible mobile phases; ensure the compound is stable in the chosen pH window.
• Avoid strong bases if the salt form is required; basification can convert to the free base and alter solubility. Filter 0.22 µm if particles persist.

Storage & Reconstitution

Item-specific storage and shipping:

• Storage Conditions: Store at −20 °C (per Product Data).
• Shipped In: Ice chest + Ice pads (per Product Data).

General reconstitution guidance for small-molecule hydrochloride salts (not item-specific):

• Solvent selection: Use anhydrous DMSO for concentrated stock solutions. If aqueous stocks are preferred, begin with water or buffer at mildly acidic pH to maintain solubility of the protonated amine.
• Concentration: Typical research stocks are 10–50 mM in DMSO. Determine solubility empirically for your lot; do not exceed visible solubility limits.
• Technique: Allow the container to warm to room temperature in a sealed state before opening to prevent moisture condensation. Weigh quickly, cap promptly, and purge with inert gas if long-term storage is planned.
• Aliquoting: Prepare single-use aliquots in amber vials or low-bind tubes to minimize adsorption and freeze–thaw cycling.

Stability practices:

• Protect from moisture and light as a precaution unless the CoA indicates insensitivity.
• Freeze–thaw: Minimize cycles; thaw on ice or at room temperature, then return unused portions to −20 °C promptly.
• Documentation: Record preparation date, solvent, concentration, and lot number on each aliquot.

If any precipitation or discoloration is observed after storage, discard the affected aliquot and prepare fresh solution. For definitive stability limits and reconstitution specifications, consult the CoA/SDS.

Structure & Identity

Brief overview: Pimicotinib hydrochloride is the hydrochloride salt of the small‑molecule research compound pimicotinib. The salt form is typically chosen to improve handling and solubility in polar media for in vitro studies.

Item-specific identifiers from Product Data:

• CAS: 2866305-19-1
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general/literature, not item-specific):

• Compound class: small organic heteroaromatic/aryl kinase-tool–type scaffold (literature characterization of pimicotinib as a targeted small-molecule research agent). Exact ring systems and stereochemistry should be confirmed from the CoA/SDS.
• Salt form: hydrochloride (protonated base with Cl− counterion), which generally increases aqueous ionization relative to the free base.

2D structural description (general):

• The hydrochloride indicates a protonated amine center(s). The free base typically contains one or more nitrogen atoms capable of forming the salt. Without the provided SMILES/InChI, the precise substitution pattern, ring count, and stereochemical elements cannot be asserted here. Please consult the product CoA or structure file for definitive connectivity and stereochemistry.

Synthetic Utility

Pimicotinib hydrochloride is an end-use small-molecule research compound and is not typically employed as a building block or reagent in organic synthesis.

General considerations (not item-specific):

• Functional groups: Hydrochloride salts indicate protonated amine functionality; nucleophilicity of the amine is suppressed in the salt form. If derivatization is desired (e.g., to generate labeled analogs), conversion to the free base may be required, followed by standard coupling chemistries (e.g., amide formation with activated esters). Such transformations should be evaluated case-by-case based on the actual structure from the CoA.
• Stability: Many complex heteroaromatics can be sensitive to strong oxidants or prolonged base; plan protection/deprotection sequences accordingly if modifying the scaffold.
• Purification: If synthetic modifications are attempted, reverse-phase preparative HPLC is often effective for polar, multi-heteroatom scaffolds and their salts.

Recommendation:

• For most laboratories, use pimicotinib hydrochloride as received for biological or analytical studies. If synthetic analogs are required, procure the corresponding intermediates or consult the primary literature for route design rather than attempting late-stage diversification on the final API-like scaffold.

Target Specificity

Item-specific target data are not provided in the Product Data for SKU P1450614.

Accordingly:

• Antigen/Target: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity/Selectivity profile: Not specified for this item; refer to CoA/Spec Sheet.
• Isotype/Clone/Epitope (if applicable): Not applicable to this small-molecule product.

Note: Any mechanistic or target discussions elsewhere in these tabs are general literature context and are not specifications for this particular lot.

Frequently Asked Questions

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.