GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Native?Native grade — protein/biomolecule in its natural (non-recombinant, non-denatured) form. Use when native structure and activity are required.≥95%(SDS-PAGE)from Human plasma; Pre-lyophilization Protein Concentration: See COA
BioReagent, Native, ≥95%(SDS-PAGE), from Human plasma; Pre-lyophilization Protein Concentration: See COA BioReagent,Native for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Prepared from fresh frozen human plasma. Human Prothrombin is a glycoprotein of molecular weight 72,000, and consists of a single polypeptide chain. Activation of Prothrombin by Factor Va, Xa and phospholipids yields the serine protease Thrombin. The human Prothrombin is >95% via SDS-PAGE and shows no reduction upon incubation with 2- mercaptoethanol.
Extinction Coefficient (1%) = 13.6
The above protein was purified from Human plasma units that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.
Specifications
Product Name
Prothrombin, CAS No.9001-26-7
Synonyms
Prothrombin from Human plasma | Human Prothrombin | Human Prothrombin (Factor II) Native Protein | Prothrombin, Human Plasma | Coagulation factor II | F2 | PT | THPH1 | RPRGL2
Grade
BioReagent, Native
Specifications & Purity
BioReagent, Native, ≥95%(SDS-PAGE), from Human plasma; Pre-lyophilization Protein Concentration: See COA
Biochemical and Physiological Mechanisms
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation
from Human plasma; Pre-lyophilization Protein Concentration: See COA
Reconstitution
We suggest hydrating the protein with a good quality water to the original volume (See COA). After hydration aliquot into a useful (one time use) size and freeze at -80℃.
Storage
Store at 2-8°C
Shipped In
Wet ice
Stability And Storage
Store at 2-8℃ long term. Upon reconstitution, it is recommended to aliquot and freeze at -80℃.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ26F0737100