GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.≥95%(SDS-PAGE)See COA
Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide co
Host species
Rat
Specificity
CD4
Immunogen
BALB/c mouse thymocytes
Positive Control
Flow: C57BL/6 Mouse spleen cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Note
This product can replace Ab094744.
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Flow Cytometry analysis of C57BL/6 Mouse splenocytes labelling CD4 with Recombinant CD4 Antibody (Ab222901) (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Ab141774) (left panel) and detected with AF 647 - conjugated affinipure goat anti-Rat IgG.
Flow Cytometry analysis of C57BL/6 Mouse splenocytes labelling CD4 (red) with Recombinant CD4 Antibody (Ab222901) at 0.2 μg/mL for 1 hour at 4°C and detected with AF 647 - conjugated affinipure goat anti-Rat IgG. Blue - Rat IgG Isotype Control Antibody (Ab141774). Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD4 Antibody (Ab222901) - SEC
The purity of Recombinant CD4 Antibody (Ab222901) is more than 95% verified by HPLC.
Application
Application
Dilution info
Flow Cytometry
≤0.5 µg per million cells in 100 µl volume
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
