GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.SUMO tag?SUMO tag grade — recombinant protein bearing a SUMO tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein. SUMO can boost expression and solubility.≥85%(SDS-PAGE)expressed in E. coli; See COA
Accession #
Q00987
Protein Tag
N-His & SUMO
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Immobilized Recombinant Human MDM2 Protein (rp184920) at 2.0 μg/mL can bind MDM2 Antibody (Ab114718) with the EC50 of 65.6 ng/mL.
Carrier Free,Bioactive,ActiBioPure™,His Tag,SUMO tag,≥85%(SDS-PAGE),expressed in E. coli; See COA ActiBioPure™,Bioactive,Carrier Free,His Tag,SUMO tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
ActiBioPure™, Bioactive, Carrier Free, His Tag, SUMO tag
Specifications & Purity
Carrier Free,Bioactive,ActiBioPure™,His Tag,SUMO tag,≥85%(SDS-PAGE),expressed in E. coli; See COA
Biochemical and Physiological Mechanisms
Double minute 2 protein (Mdm2, also known as Hdm2) is a RING-finger Ubiquitin E3 ligase that acts as a major regulator of the tumor suppressor p53. Mdm2 inhibits p53-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain
Bioactivity
Immobilized Recombinant Human MDM2 Protein (rp184920) at 2.0 μg/mL can bind MDM2 Antibody (Ab114718) with the EC50 of 65.6 ng/mL.
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ for 12 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Human MDM2 Protein (rp184920) - ELISA
Immobilized Recombinant Human MDM2 Protein (rp184920) at 2.0 μg/mL can bind MDM2 Antibody (Ab114718) with the EC₅₀ of 65.6 ng/mL.
Recombinant Human MDM2 Protein (rp184920) - SDS-PAGE
2 μg/lane of Recombinant Human MDM2 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 107.4 kDa under reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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