RNA Sample Loading Buffer - for NA electrophoresis, with ethidium bromide (50 μg/mL)

Cat. No.: R485277

GRADE & PURITY for NA electrophoresis, with ethidium bromide (50 μg/mL)

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

RNA loading buffer is used as a tracking dye during RNA electrophoresis. The RNA loading dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading.RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency.Suitable for use with formaldehyde-agarose gels used in Northern blotting procedures.RNA Sample Loading Buffer has been used as a sample loading buffer in northern blot.

Specifications

Specifications & Purity

for NA electrophoresis, with ethidium bromide (50 μg/mL)

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Frequently Asked Questions

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.