Sauton Liquid Medium Base - BioReagent

Cat. No.: S1528769

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Overview

For enrichment cultivation of Mycobacterium tuberculosis and preparation of BCG vaccine

Specifications

Specifications & Purity

BioReagent

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent

Product Line

biological_products

Application Protocols

General preparation and use (literature example; not a specification for SKU S1528769):

1. Medium preparation

• Dissolve the supplied medium base in ultrapure water to the manufacturer-recommended concentration/volume (Not specified for this item; refer to CoA/Spec Sheet).
• Adjust pH to ~7.0–7.2 with dilute HCl/NaOH.
• Add optional supplements as required by your protocol (e.g., glycerol as carbon source if not already present in the base; 0.05% Tween 80 to minimize clumping; defined trace elements). Mix until fully dissolved.
• Sterilize by autoclaving (e.g., 121 °C, 15–20 min) or sterile filtration for heat-labile components.

2. Inoculation and incubation

• Work aseptically in a biosafety cabinet appropriate for the organism. Inoculate with a standardized inoculum (e.g., McFarland-based or OD600-adjusted, per SOP).
• Incubate at species-appropriate temperature (commonly 30–37 °C). For broth cultures, shake 150–220 rpm; for plates/slants (if a solidifier is used), incubate statically.

3. Monitoring

• Measure OD600, CFU plating, or dry cell weight at intervals. Expect slow growth for some mycobacteria (days–weeks). Prevent cross-contamination and desiccation.

4. Disposal

• Decontaminate cultures and waste by validated methods (e.g., autoclave ≥121 °C per institutional biosafety policy).

Always defer to your lab’s SOPs and the product’s CoA/SDS for item-specific instructions.

Biological Roles

Sauton medium (literature) is a chemically defined growth medium designed to support the metabolism of mycobacteria.

• Carbon and energy: Typically provided by glycerol (literature), feeding central carbon metabolism (glycolysis, gluconeogenesis, TCA cycle adaptations). The defined carbon source facilitates metabolic studies (e.g., glyoxylate shunt engagement, lipid biosynthesis).
• Nitrogen: Commonly supplied as L‑asparagine (literature), entering through deamidation/transamination to provide ammonium and carbon skeletons.
• Ions and cofactors: Phosphate, magnesium, and trace iron are included to support nucleic acid synthesis, enzyme function, and respiratory processes; citrate often acts as a chelator to maintain trace metal availability (literature).
• Physiological outcomes: Supports planktonic growth and, depending on surfactant presence, clumping/cording typical of mycobacteria. Defined composition enables reproducible transcriptional and proteomic profiling under nutrient shifts.

No biological activity is claimed for this product beyond its role as a culture medium base. For SKU S1528769, component identities and concentrations are Not specified for this item; refer to CoA/Spec Sheet. Use in accordance with biosafety requirements for the organism handled.

Buffer Applications

This product is a culture medium base, not a dedicated buffer. While prepared Sauton media (literature) include phosphate/citrate species that provide some buffering capacity near neutral pH, it is not typically used as a standalone buffer system.

• If buffering is your primary need, choose defined buffers such as phosphate (pH ~6.0–8.0) or HEPES (pH ~6.8–8.2) based on your assay.
• For microbial culture in Sauton-type media, adjust pH to the protocol-specified value (commonly ~7.0–7.2 in literature) before sterilization and monitor pH drift during prolonged incubation.

Green Alternatives

Green chemistry considerations are not central to culture media selection, but sustainability can be improved via preparation and sterilization practices.

• Sterilization energy: Prefer validated low-temperature sterile filtration for heat‑labile supplements instead of extended autoclave cycles where feasible; consolidate autoclave loads to reduce energy use (institutional policies permitting).
• Waste reduction: Prepare media in volumes aligned to near-term demand to minimize expired waste. Use recyclable glass media bottles where possible.
• Component sourcing: Where formulations allow, choose components with verified sustainable sourcing (literature examples include plant-derived glycerol). This does not specify the sourcing of SKU S1528769.

Comparison (general):

• Defined media (e.g., Sauton) vs complex media (e.g., Middlebrook 7H9 with OADC): Defined media can reduce animal-derived ingredients, aiding ethical sourcing and compositional transparency; however, complex media may provide higher biomass yields, potentially reducing batch numbers. Trade off performance vs sourcing and experimental needs.

Item-specific eco attributes for SKU S1528769 are Not specified for this item; refer to CoA/Spec Sheet.

Pharmaceutical Uses

No therapeutic or clinical claims are made. For research and laboratory use only.

• Potential roles in regulated environments (general): Defined culture media are used in microbiology laboratories for research, development, and quality control activities (e.g., growth, enumeration, phenotypic assays). Any use in GMP settings requires qualification/validation by the end user.
• Pharmacopeial status: Not specified for this item; refer to CoA/Spec Sheet. Sauton formulations are not typically monographed; if a pharmacopeial grade is required, select media with explicit compendial compliance.
• Excipients/processing aids: Not applicable; this product is not intended as a formulation excipient.

Physical Properties

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• State: Multi-component culture medium base (mixture). Specific physical constants are generally not assigned.
• pH (prepared medium, literature): Sauton medium is commonly adjusted to pH ~7.0–7.2 at 25 °C (literature).
• Osmolality (prepared, literature): Moderate ionic strength typical of defined media; specific value depends on exact formulation (literature; not specified for this item).
• Density, refractive index, boiling point, melting point: Not applicable/typically not reported for complex media concentrates.
• Solubility: Designed to be water-soluble to form a clear to slightly opalescent solution after proper preparation and sterilization (general/literature). Exact solubility behavior may vary with formulation and water quality.

Important:

• Do not treat literature values as specifications. For SKU S1528769, item-specific physical specifications are Not specified for this item; refer to CoA/Spec Sheet.

Quality & Grades

• Grade: BioReagent (per Product Data). This denotes suitability for laboratory biological research applications where consistent performance and low bioburden are important, but it is not a clinical/diagnostic grade.
• Purity/assay: Not specified for this item; refer to CoA/Spec Sheet.
• Sterility/endotoxin/bioburden: Not specified for this item; refer to CoA/Spec Sheet. Users should sterilize as required for cell/microbial culture.
• Lot-to-lot consistency: For defined media such as Sauton formulations, BioReagent grade emphasizes controlled raw materials and reproducible performance; verify with your internal QC (growth curves, CFU yield, morphology) when changing lots.
• Stabilizers/preservatives: Not specified for this item; refer to CoA/Spec Sheet.
• Documentation: For regulated workflows (e.g., environmental monitoring, QC microbiology), retain the CoA/SDS and any performance certificates. This product is labeled For research use only.

Reaction & Applications

Primary application is microbiological culture, not chemical synthesis.

Research applications (literature/general):

• Cultivation of mycobacteria, including fast-growing nonpathogenic species (e.g., Mycobacterium smegmatis) and, in appropriate containment, slow-growing species (e.g., M. bovis BCG, M. tuberculosis complex) for basic research, genetics, and biochemical assays.
• Defined-medium studies: Because Sauton is chemically defined, it supports nutrient limitation and metabolic flux analyses (e.g., carbon source modulation via glycerol alternative or concentration changes; nitrogen source via L‑asparagine).
• Antibiotic susceptibility testing (research): Defined background minimizes lot variability compared with complex media; supplements (e.g., Tween 80 to reduce clumping) are often added per protocol.

Practical tips (literature):

• Disaggregation: Include a nonionic surfactant (e.g., 0.05% Tween 80) when clump-free suspensions are needed; omit when forming cords/biofilms is desired.
• Carbon/nitrogen tuning: Alter glycerol or alternative carbon sources and asparagine concentration to probe metabolic phenotypes. Maintain pH near neutral; mycobacteria are sensitive to acidic drift during prolonged incubation.
• Incubation: Typical temperatures 30–37 °C depending on species/strain; shaking (150–220 rpm) improves aeration for broth cultures.

Note: Exact supplement requirements and concentrations for SKU S1528769 are Not specified for this item; consult CoA/Spec Sheet and your protocol.

Reaction Conditions

Not a chemical reaction reagent. However, general culture conditions for Sauton-type media (literature) are provided for context:

• Temperature: Commonly 30–37 °C depending on Mycobacterium species/strain.
• Aeration: Shaking at ~150–220 rpm for broth cultures to improve oxygenation; surface/standing cultures possible for specific phenotypes.
• pH: Adjust initial pH to ~7.0–7.2 before sterilization; monitor drift during long incubations.
• Supplements: Optional Tween 80 (e.g., 0.05%) to reduce cell clumping; other supplements per experimental design (e.g., trace metals, alternative carbon sources). Concentrations are literature guidance only and not specifications for this item.
• Sterilization: Autoclave base medium as appropriate; filter-sterilize heat‑labile additives post-autoclave under aseptic conditions.

For SKU S1528769, follow your institutional SOPs and any supplier instructions. Item-specific preparation parameters are Not specified for this item; refer to CoA/Spec Sheet.

Safety & Handling

• GHS Classification, Signal Word, H‑Statements, Pictograms: Not specified for this item; refer to SDS.
• General precautions: Although medium bases are typically of low intrinsic hazard, always assess risks via the SDS. Avoid inhalation of powders/aerosols and contact with skin/eyes. Use appropriate PPE (lab coat, gloves, safety glasses) and standard microbiological practices.
• Sterility: Unless explicitly stated as sterile (not specified here), treat as non-sterile. Sterilize prepared medium by autoclaving or sterile filtration per your biosafety and experimental requirements.
• Incompatibilities: Store away from strong oxidizers and moisture ingress. Use clean, dedicated glassware to minimize contamination.
• Microbiological risk: After inoculation, cultures may contain viable microorganisms (including opportunistic or pathogenic strains). Handle in appropriate containment (e.g., BSL‑2/BSL‑3 per organism), with biohazard waste decontamination (autoclave, validated disinfectants).
• First aid (general): In case of eye/skin contact, rinse with water. If inhaled (powders/aerosols), move to fresh air. If ingested, rinse mouth. Seek medical attention if symptoms persist. Defer to SDS for authoritative guidance.
• Spills: For dry materials, avoid dust; gently sweep with minimal disturbance. For liquid media or cultures, absorb with suitable material and disinfect according to institutional biosafety procedures.

Solvent Selection

Not a solvent. Sauton Liquid Medium Base is intended for aqueous medium preparation.

• Solvent for preparation: Ultrapure water (e.g., ASTM Type I/II) is typically used to dissolve medium components and to make up to volume (literature/general practice).
• pH adjustments: Use dilute HCl or NaOH to reach the target pH specified by your protocol (commonly ~7.0–7.2 in literature for Sauton medium). Filter or autoclave after adjustment as appropriate.
• Miscibility/polarity: Not applicable; the prepared medium is an aqueous solution.

Storage & Reconstitution

• Storage conditions (as provided): Room temperature. Protect from moisture ingress and microbial contamination. Keep container tightly closed.
• Shipped: Normal (per Product Data).
• Shelf life: Not specified for this item; refer to CoA/Spec Sheet. Use-by dates should be observed once provided.
• Reconstitution/preparation: Not specified for this item; refer to CoA/Spec Sheet. As general guidance (literature), dissolve the medium base in ultrapure water to the target volume, adjust pH to ~7.0–7.2, and sterilize (autoclave or filter) prior to use. Add heat‑labile supplements after sterilization via sterile filtration.
• Aseptic handling: Prepare and dispense under aseptic conditions to maintain sterility. For multi-use containers, avoid repeated opening in humid environments; consider aliquoting.
• Post-preparation storage (general): Store prepared sterile medium at 2–8 °C, protected from light where applicable. Warm to room temperature before use. Observe for precipitation, turbidity, or color change prior to inoculation; discard if contamination or instability is suspected.

Item-specific details (concentrate strength, component list, recommended pH, sterilization method) for SKU S1528769 are Not specified for this item; refer to CoA/Spec Sheet.

Structure & Identity

Sauton Liquid Medium Base is a multi‑component culture medium formulation used in microbiology; it is not a single defined molecule.

• Chemical identity: Multi-constituent medium base (mixture)
• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Formula: Not applicable to a mixture.
• Molecular Weight: Not applicable to a mixture.
• SMILES / InChI / InChIKey: Not applicable to a mixture.

Structural features (general, literature):

• Typical Sauton medium (literature) is a chemically defined medium containing inorganic salts and defined carbon/nitrogen sources (e.g., glycerol as carbon source and L‑asparagine as nitrogen source), along with buffering components such as phosphate and organic acid chelators such as citrate.
• No stereocenters or ring systems are attributable to the “mixture” as a whole; the medium comprises multiple small molecules in aqueous solution after preparation.

Notes for users:

• As a “Liquid Medium Base,” this product is intended to be prepared according to supplier instructions (e.g., dilution, supplementation, sterilization). Exact component list and concentrations for SKU S1528769 are Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Not applicable. Sauton Liquid Medium Base is not a chemical building block or reagent for organic synthesis. It is intended for preparation of microbiological growth medium. For synthetic chemistry applications, refer to appropriate reagents and solvents in the catalog.

Target Specificity

This section does not apply. Target specificity (antigen/epitope/clone/isotype) is relevant to antibodies and affinity reagents, not to culture media. Sauton Liquid Medium Base is a general-purpose defined medium base used for cultivating mycobacteria (literature), with no antigen specificity.