SIC5-6 - Moligand™, 10 mM in DMSO , CAS No.2410846-16-9

CAS: 2410846-16-9 Cat. No.: S1496647 PubChem CID: 154827475

GRADE & PURITY Moligand™, 10 mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

SIC5-6 is a potent Separase inhibitor. Separase , a large cysteine protease, involves in chromosome segregation during mitosis and meiosis, DNA damage repair, centrosome disengagement and duplication, spindle stabilization and elongation. Separase is highly overexpressed in many solid cancers, serves as an attractive chemotherapeutic target.

Specifications

Specifications & Purity

Moligand™, 10 mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Names and Identifiers

PubChem CID

154827475

Application Protocols

No tested applications or protocols are provided for SIC5-6. For general small-molecule screening use:

• Stock preparation (generic): Dissolve in anhydrous DMSO to a verified concentration (e.g., 10–50 mM), filter if needed (0.2 µm PTFE), and aliquot to avoid repeated freeze–thaw.
• Assay dilution: Add to assay media to achieve desired final concentration while keeping DMSO ≤1% v/v (or per assay tolerance). Mix thoroughly and check for precipitation.
• Analytical QC: Confirm identity/purity by LC–MS/HPLC before and after key experiments. Record lot and preparation details for reproducibility.

For any plate-based or cellular assay, tailor the protocol to the assay platform and consult the SDS/CoA for stability and compatibility details.

Biological Roles

No biological function, target, or pathway data are provided for SIC5-6. Without structural and mechanistic information, biological roles cannot be assigned.

General considerations for small-molecule library members

• These compounds are typically used as chemical probes or screening starting points to explore biochemical pathways or cellular phenotypes in research settings.
• Any observed activity should be considered provisional until orthogonal assays, counter-screens, and selectivity profiling are performed.
• Assess liabilities such as pan-assay interference compounds (PAINS), redox activity, aggregation, or fluorescence interference using standard filters and experimental controls.

Please refer to the CoA or associated library documentation for any available target annotations or assay provenance. All uses are for research purposes only.

Buffer Applications

SIC5-6 is a small molecule from a screening library and is not itself a buffering agent. No buffer capacity, pKa, or recommended buffer systems are provided.

Practical note

• When formulating assay solutions, prepare compound stocks in DMSO (or another suitable solvent) and dilute into the assay buffer of choice (e.g., PBS, HEPES, or assay-specific media) while keeping final cosolvent low (typically ≤1%). Validate that the compound remains in solution and does not alter buffer pH at working concentrations.

Green Alternatives

With no structure or solubility profile provided, solvent and process greenness should be optimized empirically. General strategies for small-molecule library handling:

• Prefer greener solvents where feasible

  • Replace DMF/NMP with safer alternatives such as CPME, 2-MeTHF, ethyl acetate, or MeCN for sample preparation/extractions when compatible (literature guidance).
  • For biotesting dilutions, minimize DMSO to ≤0.5–1% v/v in assay to reduce solvent burden.

• Process considerations

  • Scale conservatively to limit waste; favor microplate-based solubility screens and microscale reactions.
  • Use aqueous workups with salt-assisted partitioning (e.g., brine) rather than chlorinated solvents when compatible.

• Comparative notes (general)

  • DMSO vs 2-MeTHF: DMSO excels for stock solutions but is non-volatile; 2-MeTHF is a greener, volatile reaction solvent but typically unsuitable for aqueous dilution in bioassays.
  • MeCN vs CH2Cl2: MeCN offers lower toxicity profile and LC–MS compatibility; choose over dichloromethane when chromatographic behavior allows.

Tradeoffs

• Greener solvents may reduce solubility of highly lipophilic compounds; confirm by small-scale screening.
• Stability can vary by solvent; run short-term stability tests (RT/4 °C) to verify no rapid degradation.

Note: Without item-specific data, these are general best practices; consult CoA/SDS to refine choices.

Pharmaceutical Uses

This product is supplied strictly for research use. No pharmacopeial status, excipient role, or clinical formulation guidance is provided for SIC5-6.

General context for small-molecule research compounds

• Such materials may serve as discovery tools in pre-formulation screening or as reference chemicals in analytical method development, but they are not intended for human or veterinary use.
• If formulation-style studies are desired (e.g., solubility enhancement, stability in biorelevant media), conduct them under research protocols and consult the CoA/SDS. Do not extrapolate to clinical use without extensive additional qualification.

Item-specific information such as dosage form applicability, residual solvent limits, elemental impurities, or ICH stability data: Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific physicochemical properties are not provided in the product data for SIC5-6. Where a value is not listed below, consult the CoA/Spec Sheet for authoritative specifications.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• pKa: Not specified for this item; refer to CoA/Spec Sheet.
• logP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

General handling guidance (literature/generic for screening compounds)

• Many screening compounds are provided as dry powders or DMSO solutions; if supplied as a solid, users commonly prepare concentrated stocks in anhydrous DMSO (e.g., 10–50 mM) after verifying solubility and stability.
• If hygroscopic or moisture-sensitive (unknown for this item), minimize atmospheric exposure and use desiccation and inert gas backfill.
• Conduct small-scale solubility testing across polar aprotic (DMSO, DMF, acetonitrile) and protic cosolvents (ethanol) before assay use to avoid precipitation artifacts.

Quality & Grades

• Grade/Purity: Moligand™ (as listed). Specific purity, residual solvent limits, water content, and metal limits are not provided for this item; refer to CoA/Spec Sheet.

About Moligand™ (general information)

• Moligand™ denotes a small-molecule screening/ligand library classification intended for discovery research. In this class, suppliers often provide identity confirmation (e.g., LC–MS, 1H NMR) and report purity on CoA. For this specific item, exact test methods and acceptance criteria are not specified here; consult the CoA.
• UV profile, HPLC absorbance cutoff, or LC purity windows: Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/inhibitors: None specified. If stabilizers are used, they will be listed on the CoA; absence of a listing here should not be taken as confirmation of absence.

Practical guidance

• When comparing lots, prioritize: structural identity (MS/NMR), chromatographic purity, residual solvent profile, and moisture content. Request lot-specific CoA to confirm.
• For bioassays, plate-based QC often includes mass confirmation and solubility checks in DMSO at working concentrations. Perform in-house verification before screening if critical.
• If needed for regulated work, determine if additional documentation (e.g., elemental analysis, chiral purity, endotoxin testing) is available; not specified for this item.

Reaction & Applications

Manufacturer application details are not provided beyond the Moligand™ designation. Without structure, reaction-specific behavior cannot be assigned. The following guidance reflects general use of small-molecule library members in research.

• Discovery applications (general)

  • High-throughput screening (HTS) and target-agnostic phenotypic assays: Prepare DMSO stocks, validate solubility and stability, and perform concentration–response titrations to identify chemical starting points.
  • Chemoinformatics and SAR: If analog series exist (e.g., SIC5-# series), use clustering to prioritize follow-up testing; obtain structural data from the CoA for substructure queries.

• Analytical applications

  • LC–MS characterization: Establish a stability-indicating method. Screen positive/negative ion modes and optimize mobile phase modifiers (0.1% FA or 5–10 mM ammonium acetate) to capture the parent ion and degradants.
  • Purity tracking: Monitor chromatographic purity during storage and after repeated freeze–thaw cycles.

• Lab practices for small-molecule handling

  • Solubility pre-check: Microplate solubility or nephelometry at intended assay concentrations avoids precipitation artifacts.
  • Adsorption mitigation: Use low-bind plastics or glass for lipophilic compounds; precondition pipette tips with DMSO stock.

Reaction chemistry

• Specific reaction roles (e.g., nucleophile/electrophile behavior, named-reaction participation) cannot be described without structural information. Consult the CoA/SDS for functional group details to plan any synthetic transformations.

Reaction Conditions

No reaction conditions are specified for SIC5-6. In the absence of a known structure and functional groups, it is not possible to provide meaningful temperature, catalyst, or solvent recommendations tailored to this compound.

General best practices for exploratory chemistry on unknowns or newly received compounds

• Start with analytical screening: Determine solubility and stability across a panel of solvents (DMSO, MeCN, EtOAc, MeOH, THF) and pH conditions.
• Thermal stability: Use DSC/TGA (if available) and variable-temperature NMR to identify decomposition on heating before any high-temperature reactions.
• Redox/acid–base sensitivity: Test small aliquots with mild bases (DIPEA, NaHCO3) and acids (AcOH) to gauge tolerance.
• Light/air sensitivity: If unsure, work under inert atmosphere and amber glassware until data indicate otherwise.

Expected yields and timelines (literature/general)

• For late-stage functionalization of drug-like molecules, typical small-scale reactions (e.g., amide couplings, SNAr on activated aryls, or Buchwald–Hartwig/Suzuki cross-couplings) proceed in 30–90% yield depending on functional group context; applicability here is unknown without structure.

Safety & Handling

GHS hazard classification and pictograms are not provided in the product data for SIC5-6. Always consult the item-specific Safety Data Sheet (SDS) before use.

• Signal word: Not specified for this item; refer to SDS.
• H-statements: Not specified for this item; refer to SDS.
• GHS classification: Not specified for this item; refer to SDS.
• Pictograms: Not specified for this item; refer to SDS.

General laboratory precautions (generic guidance)

• Personal protective equipment: lab coat, suitable gloves (nitrile recommended for organics), safety glasses or face shield when splashing is possible.
• Engineering controls: handle powders and solvent stocks in a fume hood; avoid aerosolization. Use dust control when weighing fine solids.
• Incompatibilities: Unknown for this item; however, avoid mixing with strong oxidizers, strong acids/bases, or reactive metals until compatibility is assessed from SDS/CoA.
• First aid overview: In case of skin/eye contact, rinse with water for at least 15 minutes and seek medical attention. If inhaled, move to fresh air. If ingested, do not induce vomiting; obtain medical advice. Follow SDS instructions.
• Spill response: Contain and collect with inert absorbent; avoid dust formation. Dispose according to institutional and local regulations.
• Waste: Treat as organic chemical waste unless SDS indicates otherwise. Segregate halogenated vs non-halogenated solvents if dissolved.

Note: For unknown peroxide-forming risk or photolability, store as directed and minimize light/air exposure until specific stability data are reviewed.

Solvent Selection

Item-specific solubility and polarity are not provided; select solvents empirically with small-scale tests. For small-molecule screening compounds, the following framework is commonly effective (general guidance):

• Primary stock preparation

  • DMSO: Default solvent for HTS/HCS due to broad solvency and assay compatibility at low final percentages (≤1–2% v/v). Verify solubility at intended stock levels (e.g., 10–50 mM).
  • Alternatives: DMF or NMP for difficult solutes; subsequently dilute into aqueous media with cosolvent control.

• Secondary dissolution and dilution

  • Aqueous buffers: Use PBS, HBSS, or assay-specific buffers with ≤1% DMSO final. Pre-warm and vortex to avoid precipitation. Consider surfactant (0.01–0.05% w/v Pluronic F-127 or Tween-20) if compatible.
  • Volatile organic solvents (MeCN, EtOH): Useful for LC-injection or when DMSO is incompatible; evaluate analyte stability.

• Solvent selection decision points

  • Chemical liabilities: If the compound contains hydrolytically labile motifs (unknown here), favor aprotic, anhydrous solvents and minimize water exposure.
  • Photolability/air sensitivity: Work ambered and under inert gas if CoA/SDS indicates sensitivity.

• Small comparison (general)

  • DMSO vs DMF: DMSO is more biocompatible; DMF may solubilize highly lipophilic molecules but is less desirable in biological assays.
  • MeCN vs EtOH: MeCN is LC–MS friendly and volatile; EtOH can aid dissolution into aqueous systems but may affect assays.

Always confirm solvent grade and blank effects on your assay or analysis.

Storage & Reconstitution

Storage

• Store at -80°C (per product data). Maintain in a non-frost-cycling, monitored ultralow freezer. Use desiccation and inert gas backfill if item is moisture/air sensitive (unknown; refer to CoA/SDS).
• Shipped on dry ice packs + cold packs to preserve integrity. Inspect upon arrival; if thawing occurred, assess by LC–MS/HPLC before use.

Handling and aliquoting

• Upon first opening, equilibrate container to room temperature in a desiccator before unsealing to minimize condensation. Immediately prepare small, single-use aliquots to avoid repeated freeze–thaw cycles.
• Protect from light if photolability is suspected; store in amber vials.

Reconstitution (general guidance for screening compounds)

• Solvent: Use anhydrous DMSO as a first choice unless the CoA specifies otherwise. Verify solubility at target stock concentration (e.g., 10–50 mM) by visual inspection and, if needed, nephelometry.
• Filtration: If particulate persists, pass through a 0.2 µm PTFE syringe filter. Avoid cellulose ester filters with strong solvents.
• Aqueous dilution: Add DMSO stock to buffered media with vigorous mixing; keep final DMSO low (≤1% v/v) to maintain assay compatibility.

Stability

• Item-specific stability, hygroscopicity, and light sensitivity: Not specified for this item; refer to CoA/Spec Sheet. Reassess purity periodically if stored long-term.

Structure & Identity

SIC5-6 (SKU: S1496647) is listed as a Moligand-class small molecule for research use. Item-specific structural identifiers are not provided in the product data.

• Chemical name: Not specified for this item; refer to CoA/Spec Sheet.
• CAS: 2410846-16-9
• PubChem CID: 154827475
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Structural features

• Item-specific functional groups, ring systems, heteroatoms, and stereochemistry: Not specified for this item; refer to CoA/Spec Sheet.

2D structure description

• A 2D line structure cannot be described without the SMILES/InChI or a structure drawing. Please consult the supplied CoA/Spec Sheet or SDS package for the definitive structural representation.

Notes

• Category: Small molecule/compound library (Moligand series).
• Identity confirmation: For this item, specific analytical methods (e.g., NMR, LC–MS) are not listed; refer to the Certificate of Analysis for definitive identity data.

Synthetic Utility

The synthetic reactivity of SIC5-6 cannot be described without structural information. No functional groups, protecting-group liabilities, or compatibility notes are provided in the product data.

General guidance if synthetic derivatization is contemplated

• Obtain the structure and functional group map from the CoA. Identify potential handles (e.g., aryl halides for cross-coupling, amines/acids for amide coupling, carbonyls for reductive amination).
• Check chemoselectivity and orthogonality relative to planned transformations; run small test reactions to assess stability to acids/bases, oxidants/reductants, and heat.
• Analytical control: Use LC–MS and NMR to monitor conversions; develop stability-indicating methods to track degradation or epimerization if stereocenters exist.

Without item-specific data, no named-reaction recommendations or protective-group strategies can be responsibly provided.

Target Specificity

No target, binding partner, or selectivity information is provided for SIC5-6. Without structural and bioactivity data, target specificity cannot be assigned. If SIC5-6 belongs to a series with known annotations, consult the series documentation or CoA for any available target profiling results.

Frequently Asked Questions

What is Moligand??

Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2410846-16-9.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.