Streptolysin O - lyophilized powder,Protein ~3 % by Lowry,25,000-50,000 U/vial, from Streptococcus pyogenes , CAS No.98072-47-0

CAS: 98072-47-0 Cat. No.: S477654 EC Number: 308-500-3 PubChem CID: 135280160

GRADE & PURITY lyophilized powder,Protein ~3 % by Lowry,25,000-50,000 U/vial, from Streptococcus pyogenes

Storage

Store at 2-8°C,Desiccated

Shipped In

Wet ice

Overview

Description

Used to create holes (reversible pores) in cholesterol-enriched plasma membranes of eukaryotic cells allowing selective permeabilization with molecules such as proteins (up to 100 kDa) and siRNAs.Streptolysin O is a toxin secreted byStreptococcus pyogenesand is a prototype member of poreforming bacterial cytolysins. It is used permeabilize cell membranes to permit cellular uptake of large or charged molecules. It is used to study macromolecule delivery. It is a potential anticancer agent and is used to study suicide cancer gene therapy.Permeabilizes membranes to permit cellular uptake of large or charged molecules.

Preparation instructions

Form

Lyophilized powder containing Tris buffer salts, sodium azide, PMSF, and EDTA.

Principle

Specifications

Specifications & Purity

lyophilized powder,Protein ~3 % by Lowry,25,000-50,000 U/vial, from Streptococcus pyogenes

Biochemical and Physiological Mechanisms

Streptolysin O possesses a single polypeptide chain with a molecular weight of f62 kDa. Streptolysin O binds to membrane cholesterol and oligomerizes to create a ring structure that consists of 45 to 50 units. The ring structure inserts into the membran

Storage

Store at 2-8°C,Desiccated

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• Pubchem Sid: 528776203
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/528776203
• WGK Germany: 3
• PubChem CID: 135280160

Chemical and Physical Properties

• Solubility: H2O: soluble 1mg/mL
• Sensitivity: Moisture Sensitive