TMB color reagent A solution (3,3',5,5'-tetramethylbenzidine)

Cat. No.: T117927

Synonyms

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate System TMB

Storage

Store at 2-8°C,Argon charged

Shipped In

Wet ice

Application

ELISA

Overview

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Specifications

Synonyms

3, 3′, 5, 5′-Tetramethylbenzidine Liquid Substrate System TMB

Storage

Store at 2-8°C, Argon charged

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Chemical and Physical Properties

• Sensitivity: Light sensitive