Trypsin-N (MS)

Cat. No.: T1523247

GRADE & PURITY Recombinant, Suitable for mass spectrometry (MS), EnzymoPure™, ≥99%(HPLC), ≥40 U/mg protein

Expression system

E. coli

Overview

MS-grade Trypsin-N protease specifically cleaves peptide bonds at the N-terminus of lysine (K) and arginine (R) residues. It is a new series of proteases with N-terminal cleavage specificity. Physical Form: MS-grade Trypsin-N protease is supplied as a lyophilized powder, containing 0.47 mg HEPES and 0.05 mg CaCl₂ per vial. Reconstitution Solution: Reconstitute with 40 μL of 50 mM zinc acetate solution before use. pH Range: Trypsin-N exhibits optimal activity at pH 7.5. Application: Trypsin-N protease specifically cleaves peptide bonds at lysine (K) and arginine (R) residues in denatured proteins. General In-Solution Digestion Protocol: Recommended Digestion Buffer: 20–50 mM HEPES or Tris buffer. Reaction Conditions: Use a protein-to-protease ratio (w/w) of 20:1. Incubate at 37°C for at least 4 hours. Tips: 1. Maximizing Activity: It is recommended that protein samples undergo denaturation and alkylation, and that the digestion reaction system and SOP are strictly followed. 2. Sensitivity: Trypsin-N is sensitive to 1 mM DTT, 0.1 mM guanidine hydrochloride, 0.8 M urea, and 5% acetonitrile, which will reduce digestion efficiency.

Specifications

Product Name

Trypsin-N (MS)

Grade

Recombinant, Suitable for mass spectrometry (MS), EnzymoPure™

Specifications & Purity

EnzymoPure™,suitable for mass spectrometry (MS),Recombinant,≥99%(HPLC),≥40 U/mg protein

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥40 U/mg protein

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Unit definition

The amount of enzyme that cleaves azocasein to reach half-maximal optical density at 366 nm under the reaction conditions: incubation at 37 °C for 30 min, pH 10.