UBP310 - ≥98%

Cat. No.: U276295

GRADE & PURITY ≥98%

Synonyms

(S)-1-(2-Amino-2-carboxyethyl)-3-(2-carboxythiophene-3-yl-methyl)-5-methylpyrimidine-2,4-dione

Storage

Store at 2-8°C,Desiccated

Shipped In

Wet ice

Overview

Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.

Specifications

Synonyms

(S)-1-(2-Amino-2-carboxyethyl)-3-(2-carboxythiophene-3-yl-methyl)-5-methylpyrimidine-2,4-dione

Specifications & Purity

≥98%

Source

Synthetic

Storage

Store at 2-8°C,Desiccated

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥98%

Chemical and Physical Properties

• Solubility: Soluble in DMSO to 100 mM

Application Protocols

No vendor-validated application protocols, concentrations, or assay conditions are provided for this item.

• Tested applications: Not specified for this item; refer to CoA/Spec Sheet.
• Recommended dilutions/concentrations: Not specified for this item; refer to CoA/Spec Sheet.
• Positive controls/assay systems: Not specified for this item; refer to CoA/Spec Sheet.

Practical guidance (general, not item-specific):

• Establish dose–response curves in your experimental system, including vehicle controls and appropriate reference compounds where available.
• Verify solution stability and absence of precipitation under assay conditions. Use single-use aliquots to avoid variability from freeze–thaw cycles.
• Document exact solvent, concentration, incubation times, and temperature to enable reproducibility.

Please consult the primary literature for protocol exemplars relevant to your model system and confirm suitability for your specific research aims.

Biological Roles

Context (literature/general):

• The term UBP310 commonly appears in the neuroscience literature as a small-molecule tool in the study of ionotropic glutamate receptors (iGluRs), particularly in parsing contributions from AMPA vs. kainate receptor subtypes to synaptic transmission and plasticity.
• iGluRs are ligand-gated ion channels mediating fast excitatory neurotransmission. Pharmacological tools that modulate these receptors enable mapping of circuit function, characterization of receptor subunit composition, and exploration of synaptic mechanisms in both central and peripheral systems.

Utility in experimental biology (general guidance):

• In acute brain slice or cultured neuron models, selective antagonists aid in isolating receptor-mediated currents under voltage clamp, reducing confounding from overlapping glutamatergic pathways.
• In heterologous expression systems (e.g., HEK cells expressing defined receptor subunits), ligands are used for potency/efficacy profiling and to investigate structure–activity relationships.

Important caveats:

• Target specificity and potency for this specific catalog item are not provided in the product data; consult primary literature and the item’s CoA/SDS for identity confirmation before inferring activity.
• Vehicle effects (e.g., DMSO) and adsorption to plastics can influence apparent potency. Employ rigorous controls and consistent handling.

Compliance:

• For research use only. Not for human or veterinary use, diagnostic procedures, or therapeutic applications.

Buffer Applications

This product is a small-molecule probe rather than a buffering reagent; it is not used to establish or control solution pH.

Practical guidance for assay buffers (general):

• Choose physiologically relevant buffers (e.g., HEPES, PBS, aCSF) compatible with your biological system and the compound’s solubility/stability profile.
• If preparing aqueous working solutions from an organic stock (e.g., DMSO), add the compound last to a fully prepared, filtered buffer to minimize precipitation.
• Maintain consistent ionic strength and pH across vehicle controls and treated samples to ensure interpretable pharmacological readouts.

Item-specific buffer properties such as pKa, buffering range, and recipe details are not applicable to this product.

Green Alternatives

Green chemistry considerations mainly relate to solvent and handling choices for this bioactive small molecule; the compound itself has no direct “greener substitute” unless your study can employ a different mechanistic probe.

• Solvent footprint (general guidance):

  • Prefer water-based buffers with minimal DMSO where assay design allows. Keep final DMSO levels as low as compatible with performance.
  • If organic stocks are required, consider ethanol as a lower-toxicity alternative to DMF when solubility permits.

• Operational improvements:

  • Use micro-aliquoting to reduce freeze–thaw cycles and waste.
  • Adopt closed, low-volume dosing techniques (positive displacement pipettes) to minimize exposure and losses.
  • Plan experiments to fully utilize thawed aliquots, reducing disposal volumes.

Illustrative comparison (general, not item-specific):

• DMSO: High solvency, widely accepted in bioassays; moderate environmental impact; typically used at ≤0.5% v/v final.

• Ethanol: Renewable routes available; good biocompatibility; lower solvency for many heteroaromatics.

• Aqueous buffers: Greenest matrix; may need pH adjustment or surfactant-free solubilization strategies.

• Procurement and packaging:

  • Consolidate orders to reduce cold-chain shipments. Store at 2–8°C desiccated as directed to prolong shelf life and avoid premature disposal.

Note: Any switch in probe identity for “greener” reasons must maintain scientific validity; confirm pharmacological profile equivalence through literature before substituting tools.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or formulation use is provided for this item. This product is supplied strictly for research use only.

General context (non-clinical):

• Small-molecule receptor ligands like those referenced as UBP-series compounds are commonly applied as in vitro/in vivo research tools to interrogate biochemical pathways, not as finished drug substances or excipients.
• Without item-specific regulatory or quality designations (e.g., USP/EP grade), this material should not be used for manufacturing or clinical applications.

Laboratory handling considerations:

• Prepare analytical documentation for internal QA (CoA, SDS, receiving inspection, and any in-house identity/purity checks) if using in GLP-like research settings.
• For formulation-like studies (e.g., dosing solutions for animal research), confirm solubility and stability in the chosen vehicle, document vehicle composition and concentration, and follow institutional approvals and regulations.

Statement of use:

• For research use only. Not for human or veterinary use.

Physical Properties

Item-specific physicochemical data have not been provided for this listing.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not applicable/unknown for a non-volatile small-molecule solid; specific value not provided.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• LogP, pKa, refractive index: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general guidance (not item-specific):

• Many neurotransmission tool compounds are crystalline solids of low aqueous solubility; DMSO is commonly used to prepare concentrated stock solutions (e.g., 10–50 mM), followed by dilution into physiological buffers immediately before use to minimize precipitation. Actual solubility for this product should be verified experimentally at your conditions.
• If the compound bears ionizable groups (common among iGluR ligands), apparent solubility can vary with pH and ionic strength; maintain consistent buffer composition during assays to ensure reproducibility.

Practical recommendations:

• Perform a small-scale solubility screen (water/PBS, DMSO, DMF, ethanol) at your target concentration and temperature.
• Filter clarify working solutions (0.22 µm) if compatible to remove particulates and assess stability over the intended assay time course.
• For quantitative work, determine extinction coefficient or use weight/volume confirmation rather than OD estimates unless literature-validated for the exact form you are using.

Quality & Grades

Item-specific grade and purity are not stated in the provided product data.

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet for assay, impurity profile, and analytical methods (e.g., HPLC/UPLC area %).
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.

How to interpret typical documentation (general guidance):

• Research grade small molecules are commonly qualified by one or more orthogonal techniques (e.g., 1H NMR, LC-MS) and an area% purity by HPLC. Confirm the analytical cutoff, detection wavelength(s), and gradient used when comparing lots.
• If a stabilizer or counterion is present (e.g., TFA salt, water of crystallization), it will be listed on the CoA and impacts MW for solution preparation. No stabilizer information is provided in this listing; verify on the CoA.
• UV profile: If using HPLC/UPLC detection, low baseline absorbance and a clean chromatogram are desired; specific UV cutoff or absorbance characteristics are not specified for this item.

Practical quality controls:

• On receipt, inspect packaging integrity, label, and lot. Retain CoA and SDS with the vial.
• Optionally run a quick identity/purity check under your in-house conditions (short LC-MS method) to align with the supplier’s results.
• For quantitative biology, consider aliquoting a master DMSO stock, verifying concentration by quantitative NMR or UV if an extinction coefficient is known for the exact form.

Reaction & Applications

This product is offered as a small-molecule research tool rather than a synthetic reagent; it is not typically employed as a reagent or catalyst in preparative organic reactions.

Research applications (literature/general, not item-specific):

• The name UBP310 is widely cited in neuroscience for probing glutamatergic signaling, especially kainate-type ionotropic glutamate receptor function, in electrophysiology, slice pharmacology, and receptor profiling assays.
• Typical experimental contexts include patch-clamp recordings, field potential measurements, Ca2+ imaging, and ligand competition studies, where selective antagonists/ligands help parse receptor subtype contributions.

Practical considerations:

• Prepare concentrated DMSO stocks to minimize vehicle in functional assays; confirm compound stability under light, air, and buffer conditions relevant to your protocol.
• Use matched vehicle controls and perform concentration-response studies to map dynamic range and potential off-target effects.
• If binding or functional assays are temperature sensitive, equilibrate solutions to assay temperature to avoid solubility artifacts.

Not applicable here:

• Named synthetic reactions (e.g., Grignard, cross-coupling) and catalyst applications are not relevant to the typical use of a finished bioactive probe like UBP310. For chemical synthesis of analogs, consult primary literature with fully specified structures and conditions.

Reaction Conditions

This listing is not intended for use as a reagent in named organic reactions; no item-specific reaction conditions are applicable.

General operational conditions for biological use (literature-oriented, not item-specific):

• Stock solutions: Often prepared in anhydrous DMSO and stored as single-use aliquots at low temperature to avoid repeated freeze–thaw. Exact concentrations and stability must be established experimentally for this item.
• Working solutions: Prepared freshly by diluting stock into physiological buffers at the desired final concentration with minimal vehicle content.
• Light and moisture: Many bioactive heteroaromatics benefit from protection from light and humidity; this item’s storage guidance (2–8°C, desiccated) supports this practice.

If you plan chemical reactions involving this compound (e.g., for conjugation or prodrug formation), obtain the exact structural information and consult primary literature for conditions relevant to the functional groups present.

Safety & Handling

Authoritative safety information resides in the Safety Data Sheet (SDS). The product data provided here do not include GHS details for this item.

• GHS/labeling for this item:
  • Signal word: Not specified for this item; refer to SDS.
  • Hazard statements: Not specified for this item; refer to SDS.
  • Pictograms: Not specified for this item; refer to SDS.
  • Classification: Not specified for this item; refer to SDS.

General laboratory precautions (good practice for small organic research compounds):

• Wear appropriate PPE: lab coat, safety glasses, and chemical-resistant gloves. Avoid inhalation of dust or aerosols and prevent skin/eye contact.
• Use in a fume hood during weighing, dissolution, and aliquoting to control exposure.
• Avoid incompatible reagents and strong oxidizers until specific compatibility is confirmed by SDS/CoA.

First aid (general guidance; defer to SDS):

• Inhalation: Move to fresh air; seek medical attention if symptoms persist.
• Eye/skin contact: Rinse with water for at least 15 minutes; remove contaminated clothing; obtain medical attention if irritation continues.
• Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.

Waste and spill response:

• Collect spills with inert absorbent; place in a suitable container for hazardous waste disposal per institutional and local regulations.

Special notes:

• Product is shipped on wet ice and should be stored desiccated at 2–8°C, suggesting moisture sensitivity and/or hydrolytic or oxidative liability. Minimize freeze–thaw and atmospheric exposure.

Solvent Selection

Item-specific solubility and solvent recommendations are not provided for this product. The guidance below reflects common practice for small-molecule research probes.

• Primary stock solvents (general):

  • DMSO: Preferred for many heteroaromatic/ionizable ligands due to broad solvating power and compatibility with biological assays at low final %.
  • DMF: Useful alternative when DMSO is unsuitable; check biological compatibility.
  • Ethanol or methanol: Potential co-solvents for rapid dissolution, often combined with aqueous buffer for immediate use.

• Aqueous compatibility:

  • If the compound is weakly soluble in water, prepare a concentrated DMSO stock (e.g., 10–50 mM) and dilute into buffer with vigorous mixing; keep final DMSO ≤0.1–0.5% v/v where assay permits. Determine your assay’s vehicle tolerance empirically.

• pH and salt effects:

  • For ionizable compounds, solubility can be enhanced by adjusting pH within the stability window; however, without item-specific pKa/stability, pH adjustments should be evaluated on a small scale.

• Quick comparison (general):

  • DMSO: Highest solvency; biological tolerance required at low %.
  • Ethanol: Lower solvency; wider bio-compatibility at modest %.
  • PBS/HBSS: Most physiologically relevant; may require co-solvent.

• Practical tips:

  • Warm gently (room temperature to 37°C) and vortex/sonicate to aid dissolution; avoid prolonged heating.
  • Filter clarify working solutions if compatible. Prepare fresh dilutions immediately prior to experiments to limit precipitation.

Storage & Reconstitution

Storage conditions for this item are specified; reconstitution guidance below is general and should be adapted after confirming the compound’s exact identity and solubility.

• Storage (item-specific):

  • Store at 2–8°C, desiccated.
  • Shipped on wet ice.
  • Minimize exposure to moisture and ambient air; recap promptly. Protect from prolonged light if feasible.

• Stability: Not specified for this item; refer to CoA/Spec Sheet for any shelf-life or stability testing data.

• Reconstitution (general guidance for small-molecule probes):

  • Allow vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.
  • Prepare a concentrated stock solution in a suitable anhydrous solvent (commonly DMSO) at a known concentration. Mix thoroughly by vortexing and, if needed, brief sonication. Do not heat above ambient-to-37°C without confirming stability.
  • Sterile-filter if the application requires and the compound is compatible with filtration.
  • Aliquot stocks into single-use portions in inert, low-binding tubes. Label with compound name (UBP310), SKU (U276295), lot, solvent, and concentration.
  • Store aliquots at 2–8°C or lower per stability data; avoid repeated freeze–thaw cycles. Prepare fresh working solutions in buffer immediately prior to use.

• Disposal: Follow institutional hazardous waste procedures for organic chemicals and DMSO-containing solutions.

For definitive instructions, consult the lot-specific CoA and SDS.

Structure & Identity

Overview: UBP310 is supplied as a research-use small molecule tool compound. Specific structural identifiers for this catalog entry have not been provided in the product data.

• Item-specific identifiers:

  • Chemical name: Not specified for this item; refer to CoA/Spec Sheet.
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

• Literature context (general, not item-specific):

  • The name “UBP310” is commonly used in the neuroscience literature for a small-molecule glutamatergic tool compound employed to probe ionotropic glutamate receptors. Exact substitution patterns and formal identifiers can vary by supplier; always verify using the CoA/SDS accompanying this lot.

• Structural features (general description, literature):

  • UBP-series compounds in the literature typically contain heteroaromatic scaffolds with multiple hydrogen-bonding functionalities that underpin receptor binding. Without item-specific identifiers, the precise ring system(s), functional groups, and stereochemistry for this listing cannot be stated.

• Practical note:

  • For unambiguous citation, include the SKU (U276295) and “UBP310, Aladdin Scientific” in methods sections and cross-check lot-specific identity data (NMR/MS) on the CoA where provided.

Synthetic Utility

UBP310, as supplied, is intended to be used as a finished bioactive probe rather than as a synthetic intermediate or building block.

• Item-specific functional groups and reactivity: Not specified for this item; refer to CoA/Spec Sheet for structural details if derivatization is contemplated.

General comments (when structural details are known):

• If a research team intends to generate analogs, typical approaches involve late-stage diversification of heteroaromatic cores and modulation of hydrogen-bond donors/acceptors that govern receptor binding. However, such chemistry requires explicit structural information not provided here.
• Purification of closely related analogs often relies on reverse-phase HPLC with volatile buffers; ensure UV-Vis properties are suitable for detection, or use MS-directed fractionation.

Practical recommendation:

• Treat this product as a reference compound or control ligand. For synthetic campaigns, source or determine the full structure and confirm identity with comprehensive spectroscopic data before planning transformations.

Target Specificity

No target or specificity information is provided in the product data for this catalog item.

• Antigen/target name: Not specified for this item; refer to CoA/Spec Sheet.
• Mechanism/class: Not specified for this item; refer to CoA/Spec Sheet.
• Species/selectivity data: Not specified for this item; refer to CoA/Spec Sheet.

Note: If using UBP310 as a pharmacological tool, consult primary literature for reported target profiles and validate activity in your assay system. Do not assume equivalence without confirming identity and potency for the specific lot.

Frequently Asked Questions

What is the purity of this product?

This product is supplied at ≥98% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at 2–8 °C, desiccated. Keep the container tightly closed with desiccant — the material is moisture-sensitive.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.