Universal Antibody Diluent Buffer - BioReagent, Suitable for Flow Cytometry, Suitable for Immunofluorescence(IF), ready-to-use, Azide Free

Cat. No.: P187048

GRADE & PURITY BioReagent, Ready-to-use, Azide Free, Suitable for Immunofluorescence(IF), Suitable for Flow Cytometry

Synonyms

Antibody Diluent | Antibody Diluent Antibody Dilution Buffer | PRIMARY ANTIBODY DILUENT | Universal Antibody Diluent Buffer, Ready-to-use, with BSA | Flow Cytometry Antibody Diluent Buffer

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

ELISA, Flow Cytometry, IF/ICC, WB

Overview

Universal Antibody Diluent Buffer is a ready to use PBS based buffer containing Proclin300 and Bovine Serum Albumin (BSA) that has been developed for stability during long-term storage and for improved antibody performance. This product can be used for the dilution and formulation of primary or secondary antibodies, including biotinylated antibodies and fluorescent labeled antibodies. This product has been tested to work in WB, IF/ICC, ELISA and Flow Cytometry.
Instructions for Use：
Perform appropriate fold dilutions of primary or secondary antibodies directly with Universal Antibody Diluent Buffer according to the antibody instructions for use. Antibodies preserved in Universal Antibody Diluent Buffer are recoverable for storage at 4℃ and can be used repeatedly multiple times until ideal results are not achieved then discarded.

Specifications

Synonyms

Antibody Diluent | Antibody Diluent Antibody Dilution Buffer | PRIMARY ANTIBODY DILUENT | Universal Antibody Diluent Buffer, Ready-to-use, with BSA | Flow Cytometry Antibody Diluent Buffer

Specifications & Purity

BioReagent, Suitable for Flow Cytometry, Suitable for Immunofluorescence(IF), ready-to-use, Azide Free

Stability And Storage

The solution should be stored undiluted between 2°C and 8°C for 12 months. Do not freeze.

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Azide Free, BioReagent, Ready-to-use, Suitable for Flow Cytometry, Suitable for Immunofluorescence(IF)

Images

IF/ICC: Universal Antibody Diluent Buffer (P187048)

Universal Antibody Diluent Buffer (P187048) - IF/ICC
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Streptavidin protein (FITC) (rp155876) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). The biotinylated antibody and FITC labeled antibody were preserved in Universal Antibody Diluent Buffer (P187048). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Universal Antibody Diluent Buffer (P187048)

Universal Antibody Diluent Buffer (P187048) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD3 Antibody (PE) (Ab214237) at 5μL/test for 1 hour at 4°C. The PE labeled antibody was preserved in Universal Antibody Diluent Buffer (P187048). Left panel - Mouse IgG (PE) (Ab180308).

Frequently Asked Questions

What does Suitable for Immunofluorescence(IF) mean?

Suitable for Immunofluorescence(IF) indicates the product has been validated for use in immunoassays such as ELISA, IF, IHC and flow cytometry. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.