BECN1 Mouse mAb - Anticuerpo primario, specific to BECN1, IgG1

Cat. No.: Ab090922

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0mg/mL

Application

ELISA, Flow Cytometry, IHC, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

2A4

Conjugation

Unconjugated

Descripción general

Mouse anti Human BECN1 Antibody, Monoclonal (2A4), could be used for WB, IHC, Flow Cytometry, ELISA and so on.

Specifications

Product Name

BECN1 Mouse mAb - Anticuerpo primario, specific to BECN1, IgG1

Sinónimos

Coiled-coil myosin-like BCL2-interacting protein | Protein GT197 | APG6 antibody | ATG 6 antibody | ATG6 antibody | ATG6 autophagy related 6 homolog antibody | Bcl-2-interacting protein beclin antibody | Beclin 1 (coiled coil moesin like BCL2 interacting

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0mg/mL

Mecanismos bioquímicos y fisiológicos

Plays a central role in autophagy. Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved

Especies hospedadoras

Ratón

Especificidad

BECN1

Inmunógeno

Recombinant protein of human BECN1 (AA 51-248).

Control positivo

WB: Hela, A431, MCF-7, Raji, Jurkat and SKBR-3 cell lysate; IHC: breast cancer tissues and liver cancer tissues; Flow: Raji cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150kDa

Método de purificación

Protein G purified

Concentración

1.0mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: BECN1 Mouse mAb (Ab090922)

BECN1 Mouse mAb (Ab090922) - Western Blot
All lanes: BECN1 Mouse mAb (Ab090922) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa

Flow Cytometry: BECN1 Mouse mAb (Ab090922)

BECN1 Mouse mAb (Ab090922) - Flow Cytometry
Flow cytometric analysis of Raji cells using BECN1 Mouse mAb (Ab090922)
and negative control (purple).

IHC: BECN1 Mouse mAb (Ab090922)

BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.

IHC: BECN1 Mouse mAb (Ab090922)

BECN1 Mouse mAb (Ab090922) - IHC
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using BECN1 Mouse mAb (Ab090922) at a dilution of 1/200 with DAB staining.

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/2000;

• IF/ICC:

  1/50-1/200 ;

• ELISA:

  1/10000 ;

• IHC:

  1/200-1/1000 ;

• Flow Cytometry:

  1/200-1/400 ;

Objetivos asociados (humanos)

BECN1 Tbio Beclin-1 (0 Activities)

Discover Target: BECN1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción