GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Ex:565nm?Fluorescence excitation maximum at 565 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal.Em:575nm?Fluorescence emission maximum at 575 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk.0.5 mg/mL
Transfected k562 cells expressing the extracellular domain of murine CD19.
Positive Control
Flow: Mouse splenocytes.
Conjugation
PE
Excitation (nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
Grade
Em:575nm, Ex:565nm, ExactAb™, Validated
Isotype
Rat IgG2a
Light Chain Type
kappa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
CD19 Rat mAb (PE) (Ab185394) - Flow Cytometry Flow cytometry analysis of CD19 expression by mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD19 Rat mAb (PE) (Ab185394) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (PE) (Ab180312).
CD19 Rat mAb (PE) (Ab185394) - Flow Cytometry Flow cytometry analysis of CD19 expression by mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD19 Rat mAb (PE) (Ab185394) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.
Flow Cytometry
≤0.1 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.1 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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