Recombinant CD19 Antibody (Biotin) - Anticuerpo primario, specific to Mouse CD19

Cat. No.: Ab213879

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

consejo

Reactive species

Ratón

Isotype

Rat IgG2a

Clone Number

P99G09

Conjugation

Biotin

Descripción general

Recombinant CD19 Antibody, clone P99G09, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD19 Antibody (Biotin) - Anticuerpo primario, specific to Mouse CD19

Sinónimos

Antibody deficiency due to defect in CD19 antibody | Antibody deficiency due to defect in CD19, included antibody | AW495831 antibody | B lymphocyte antigen CD19 antibody | B lymphocyte surface antigen B4 antibody | B-lymphocyte antigen CD19 antibody | B-

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Especies hospedadoras

consejo

Especificidad

Mouse CD19

Inmunógeno

Mouse CD19-expressing K562 human erythroleukemia cells

Control positivo

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugación

Biotin

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: Recombinant CD19 Antibody (Biotin) (Ab213879)

Recombinant CD19 Antibody (Biotin) (Ab213879) - Flow Cytometry
Flow cytometry analysis of CD19 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD19 Antibody (Biotin) (Ab213879) at 0.04μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310).

Flow Cytometry: Recombinant CD19 Antibody (Biotin) (Ab213879)

Recombinant CD19 Antibody (Biotin) (Ab213879) - Flow Cytometry
Flow cytometry analysis of CD19 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD19 Antibody (Biotin) (Ab213879) 0.04μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Objetivos asociados (humanos)

CD19 Tclin B-lymphocyte antigen CD19 (0 Activities)

Discover Target: CD19

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)