CD27 Rat mAb - Primary antibody, specific to CD27, Rat IgG2a

Cat. No.: Ab094343
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) See COA
Application
Animal Model, Flow Cytometry, Functional Assay, IP, Stimulation
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2a
Clone Number
RM27-3E5
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab094343-10μg
10
$67.90
50μg
Ab094343-50μg
4
$199.90
100μg
Ab094343-100μg
2
$299.90
1mg
Ab094343-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$1,999.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rat anti Mouse CD27 Antibody, Monoclonal (RM27-3E5), could be used for Flow, IP, in vivo CD27 stimulation, in vitro CD27 stimulation and so on.
Application
Flow: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
in vivo CD27 stimulation: Use at an assay dependent concentration.
in vitro CD27 stimulation: Use at an assay dependent concentration.
Protein Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Specifications

Product Name
CD27 Rat mAb - Primary antibody, specific to CD27, Rat IgG2a
Synonyms
S152 | Tnfrsf7 | CD27 antigen | CD27 molecule | CD27 | MGC20393 | S152CD27L receptor | T cell activation antigen CD27 | T cell activation antigen S152 | T14 | TNFRSF7 | TNFRSF7T-cell activation antigen CD27 | Tp55 | Tumor necrosis factor receptor superfam
Specifications & Purity
Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA
Host species
Rat
Specificity
CD27
Immunogen
Mouse CD27-human IgG1 Fc fusion protein
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, PBS Only, Validated
Isotype
Rat IgG2a
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at 2-8°C,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.
Images

CD27 Rat mAb (Ab094343) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD27 with CD27 Rat mAb (Ab094343) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

CD27 Rat mAb (Ab094343) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD27 (red) with CD27 Rat mAb (Ab094343) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
IP

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vivo CD27 stimulation

Use at an assay dependent concentration.

in vitro CD27 stimulation

Use at an assay dependent concentration.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
CD27 Tbio CD27 antigen (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0607316Certificate of AnalysisApr 07, 2026 Ab094343
ZJ24F0607317Certificate of AnalysisApr 07, 2026 Ab094343
ZJ24F0607318Certificate of AnalysisApr 07, 2026 Ab094343
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View Azide Free grade guide → View PBS Only grade guide →

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