Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The experimental value of lectin-based detection lies in converting glycan recognition into signals that can be localized, compared, and validated. Histochemistry, ELISA, and Western blot correspond to three different levels of information output: spatial distribution analysis, solid-phase ...
Immunoprecipitation (IP) is a classical biochemical method that enables selective enrichment of target proteins through antibody–antigen specific recognition. It is commonly used to obtain target-protein inputs with a higher signal-to-noise ratio from complex lysates, supporting Western ...
Immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) are two foundational approaches in protein research used for selective enrichment of a target protein and for validating protein–protein interactions, respectively. Immunomagnetic beads immobilized with antibodies or nanobodies ...
The success of an experiment relies on high-quality sample preparation and precise, reliable quantitative analysis. To support your protein research, Aladdin has meticulously developed a series of reagents and kits tailored to critical steps of protein workflows. From accurate protein ...
Protein blotting (WB) is widely used to analyze the expression of specific proteins in cell or tissue extracts. Western blot experiments are time-consuming, and their success has a significant impact on research progress.
When choosing a secondary antibody for western blotting, one of the main selection criteria is the species of the primary antibody that the secondary antibody binds to.
Immunoprecipitation (IP) experiments are important techniques in biochemistry and molecular biology for studying protein interactions and protein expression status.
The Western Blot experiment can be said to be the most basic experiment in the biology major. However, although WB experiments are done every day, a normal high-definition result picture is not something you can have whenever you want.
Western experimental procedure
Western Blot FAQ Analysis
Immunoprecipitation (IP) is a molecular biology technique based on antigen-antibody specific binding, which is used to selectively separate and enrich target proteins from complex biological samples. The core of this technology is to capture the protein of interest using specific antibodies on a ...
Western blot is used to identify large molecular weight antigens (usually proteins) that can interact with specific antibodies and to determine the size of the antigen. Proteins are first separated by SDS-PAGE and then transferred by electrophoresis to a solid support, which includes ...
The basic principles and operation steps of Western Blot experiments of histones are similar to those of other conventional proteins, both of which rely on specific antibodies to detect the presence and expression of target proteins. However, due to the special properties and functions of ...
Phosphoprotein western blot is a widely used technique for detecting and analyzing the phosphorylation status of proteins, which is essential for understanding various biological processes and signal transduction pathways. Some common application scenarios include signal transduction research, ...
There are many kinds of Western Blot internal reference antibodies, such as β-actin, β-tubulin, GAPDH, Lamin B, etc., so how should we do the selection for our own experiments? Below is a brief introduction to the principles that should be followed in selecting internal reference antibodies.
SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used technique to separate proteins based on size. By denaturating the protein and giving it a uniform negative charge, the protein migrates to the positive pole under the application of an electric field and is ...
In order to facilitate the experiments of scientific researchers, we have sorted out the buffer formulations commonly used in laboratory basic research in molecular biology, protein biology, microbiology and other aspects for researchers to facilitate access and use.
Techniques often explored alongside immunological experiments.
