CD4 Mouse mAb (Biotin) - Anticuerpo primario, specific to CD4, Mouse IgG1

Cat. No.: Ab181157

GRADE & PURITY Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

RPA-T4

Conjugation

Biotin

Descripción general

Application:
Flow: ≤0.5 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

CD4 Mouse mAb (Biotin) - Anticuerpo primario, specific to CD4, Mouse IgG1

Sinónimos

CD_antigen: CD4 | CD4 antigen (p55) | CD4 antigen | CD4 molecule | CD4 receptor | CD4 | CD4mut | T-cell surface antigen T4/Leu-3 | T-cell surface glycoprotein CD4 | CD 4 antibody | CD4 (L3T4) antibody | CD4 antibody | CD4 antigen (p55) antibody | CD4 anti

Especificaciones y pureza

Validated, 0.5 mg/mL

Especies hospedadoras

Ratón

Especificidad

CD4

Control positivo

Flow: Human peripheral blood lymphocytes

Conjugación

Biotin

Grado

Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD4 Mouse mAb (Biotin) (Ab181157)

CD4 Mouse mAb (Biotin) (Ab181157) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD4 Mouse mAb (Biotin) (Ab181157) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).

Flow Cytometry: CD4 Mouse mAb (Biotin) (Ab181157)

CD4 Mouse mAb (Biotin) (Ab181157) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD4 Mouse mAb (Biotin) (Ab181157) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

Aplicación

Dilution info

Flow Cytometry

≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Objetivos asociados (humanos)

CD4 Tclin T-cell surface glycoprotein CD4 (0 Activities)

Discover Target: CD4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD4 Mouse mAb (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.