CD8 alpha Rat mAb (AF488) - Anticuerpo primario, specific to CD8 alpha

Cat. No.: Ab190216

CALIDAD & PUREZA ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

consejo

Reactive species

Ratón

Isotype

Rat IgG2a

Clone Number

53-6.7

Conjugation

AF488

Descripción general

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow Cytometry.

Especificaciones

Product Name

CD8 alpha Rat mAb (AF488) - Anticuerpo primario, specific to CD8 alpha

Sinónimos

Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | CD8 antigen, alpha polypeptide (p32) | CD8 | CD8a molecule | CD8A | Leu2 T-lymphocyte antigen | LEU2 | MAL | OKT8 T-cell antigen | p32 | T cell co-receptor | T8 T-cell antigen | T-cell antigen Leu2 | T-cell

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Especies hospedadoras

consejo

Especificidad

CD8 alpha

Inmunógeno

Mouse Spleen Cells or Thymocyte Membranes

Control positivo

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugación

AF488

Excitación (nm)

Blue Laser(488nm)

Emisión (nm)

525nm

Ex/Em(nm)

Ex:490nm;Em:525nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD8 alpha Rat mAb (AF488) (Ab190216)

CD8 alpha Rat mAb (AF488) (Ab190216) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (AF488) (Ab190216) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF488) (Ab190240).

Flow Cytometry: CD8 alpha Rat mAb (AF488) (Ab190216)

CD8 alpha Rat mAb (AF488) (Ab190216) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (AF488) (Ab190216) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

Aplicación

Dilution info

Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Objetivos asociados (humanos)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD8 alpha Rat mAb (AF488)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.