CD8 alpha Rat mAb (PE) - Anticuerpo primario, specific to CD8 alpha

Cat. No.: Ab190209
Disponible para pedir
GRADE & PURITY ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. Ex:565nm ? Fluorescence excitation maximum at 565 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:575nm ? Fluorescence emission maximum at 575 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 0.5 mg/mL
Application
Flow Cytometry
Host species
consejo
Reactive species
Ratón
Isotype
Rat IgG2a
Clone Number
53-6.7
Conjugation
PE
★
Size
Alemania (EU)
USA*
Price
Qty
10μg
Ab190209-10μg
—
2 Disponible
19,00€
25μg
Ab190209-25μg
—
2 Disponible
36,36€
100μg
Ab190209-100μg
—
1 Disponible
97,10€
Enter a quantity for the sizes you want to add.
🧪
Why this grade

ExactAb™, Validated, Ex:565nm, Em:575nm, 0.5 mg/mL Em:575nm,Ex:565nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Rat anti Mouse CD8 alpha Antibody, Monoclonal (53-6.7), could be used for Flow, ICC/IF, WB, in vivo CD8+ T cell depletion and so on.

Specifications

Product Name
CD8 alpha Rat mAb (PE) - Anticuerpo primario, specific to CD8 alpha
Sinónimos
Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen: CD8a | PE Anti-Mouse CD8a (53-6.7) | PE Anti-Mouse CD8a Antibody[53-6.7] | CD8a Monoclonal Antibody (53-6.7), PE | PE anti-mouse CD8a Antibody | T8/Leu-2 T-lymphocyte differentiation antigen antibody | T8 T cell a
Especificaciones y pureza
ExactAb™, Validated, Ex:565nm, Em:575nm, 0.5 mg/mL
Mecanismos bioquímicos y fisiológicos
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Especies hospedadoras
consejo
Especificidad
CD8 alpha
Inmunógeno
Mouse Spleen Cells or Thymocyte Membranes
Control positivo
Flow: C57BL/6 mouse splenocytes.
Conjugación
PE
Excitación (nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emisión (nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
Grado
Em:575nm, Ex:565nm, ExactAb™, Validated
Tipo de cadena ligera
kappa
Método de purificación
Protein G purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Imágenes

CD8 alpha Rat mAb (PE) (Ab190209) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (PE) (Ab190209) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

CD8 alpha Rat mAb (PE) (Ab190209) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (PE) (Ab190209) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación
Aplicación
Dilution info
Flow Cytometry

≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeFechaArticulo
ZJ24F1012895Certificate of AnalysisMay 20, 2026 Ab190209
ZJ24F1012896Certificate of AnalysisMay 20, 2026 Ab190209
ZJ24F1012897Certificate of AnalysisMay 20, 2026 Ab190209
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:565nm grade guide → View Em:575nm grade guide →

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