IQCD Mouse mAb: Specificity, Applications & Protocol Guide

IQCD Mouse mAb is a monoclonal antibody raised in mouse that targets the IQ motif-containing protein D (IQCD), offering researchers a highly specific tool for detecting endogenous IQCD across a range of immunological and cell biology applications.

Product Name

IQCD Mouse mAb

SKU

Ab351263

Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Formulation

PBS only

Reference Code

absea#KC-45402

Target Protein

IQCD (IQ Motif Containing D)

Target Biology: What Is IQCD?

IQCD (IQ Motif Containing D) is a protein characterized by the presence of one or more IQ motifs — short amino acid sequences (approximately 25 residues) that mediate binding to calmodulin and related calcium-sensing proteins in a calcium-independent or calcium-dependent manner. IQ motif-containing proteins are broadly implicated in signal transduction, cytoskeletal organization, and intracellular calcium signaling pathways.

While the precise biological function of IQCD remains an active area of investigation, proteins within the IQ motif family are known to play roles in cell motility, neuronal signaling, and organelle positioning. Researchers studying calmodulin-interacting proteomes or intracellular signaling networks may find IQCD a relevant target for expression profiling and functional studies.

Product Specifications & Formulation

The IQCD Mouse mAb (SKU: Ab351263) is supplied as a purified monoclonal antibody produced in mouse. The antibody is formulated in phosphate-buffered saline (PBS) only, without the addition of carrier proteins such as BSA or preservatives such as sodium azide unless otherwise specified on the vial label. Researchers should verify the exact concentration and volume supplied by consulting the accompanying Certificate of Analysis (CoA).

Key specifications at a glance:

• Host: Mouse
• Clonality: Monoclonal
• Formulation: PBS only
• Reference: absea#KC-45402
• Storage: Store at –20 °C for long-term storage; avoid repeated freeze-thaw cycles. Short-term storage at 4 °C is acceptable (typically up to 1 month).

Always consult the product datasheet and CoA for lot-specific information.

Recommended Applications & Usage Guidelines

Monoclonal antibodies raised against specific protein targets such as IQCD are typically validated for one or more of the following applications. Users should confirm validated applications via the product datasheet, as performance may vary by lot and experimental system:

• Western Blot (WB): Detect denatured IQCD protein in cell or tissue lysates separated by SDS-PAGE. Standard recommended dilution ranges for monoclonal antibodies in WB are typically 1:500–1:2000; confirm on datasheet.
• Immunofluorescence (IF): Visualize subcellular localization of IQCD in fixed cells. Optimize fixation method (paraformaldehyde vs. methanol) empirically.
• Immunoprecipitation (IP): Enrich endogenous IQCD from cell lysates for interaction studies or downstream mass spectrometry analysis.
• ELISA: Quantify IQCD levels in solution-phase formats.

Because this antibody is formulated in PBS only (no BSA), it is particularly suitable for direct conjugation workflows, including fluorophore labeling or enzyme conjugation, without the need to remove carrier proteins beforehand.

General Protocol: Western Blot with IQCD Mouse mAb

The following is a general Western blot protocol for use with IQCD Mouse mAb (Ab351263). Optimize conditions empirically for your cell or tissue system.

1. Sample preparation: Lyse cells or homogenize tissue in RIPA buffer supplemented with protease inhibitor cocktail. Determine protein concentration by BCA or Bradford assay. Load 20–40 µg total protein per lane.
2. SDS-PAGE: Resolve proteins on an appropriate percentage polyacrylamide gel (typically 10–12% for most IQ motif proteins). Transfer to PVDF or nitrocellulose membrane.
3. Blocking: Block membrane in 5% non-fat dry milk or 3–5% BSA in TBST for 1 hour at room temperature.
4. Primary antibody incubation: Dilute IQCD Mouse mAb in blocking buffer (start with 1:1000; optimize as needed). Incubate overnight at 4 °C with gentle rocking.
5. Washing: Wash membrane 3 × 10 minutes with TBST.
6. Secondary antibody: Incubate with appropriate HRP-conjugated anti-mouse IgG secondary antibody (1:5000–1:10000) for 1 hour at room temperature.
7. Detection: Wash 3 × 10 minutes with TBST, then develop with ECL substrate. Image using film or digital imaging system.

Note: Specific dilution and incubation conditions should be verified against the product datasheet and CoA for each lot.

Storage, Handling & Quality Assurance

Storage conditions: The IQCD Mouse mAb (Ab351263) should be stored at –20 °C upon receipt for long-term stability. Aliquoting the antibody into single-use volumes is strongly recommended to minimize degradation caused by repeated freeze-thaw cycles. For short-term use (up to one month), storage at 4 °C is acceptable.

Handling: Centrifuge the vial briefly before opening to collect contents at the bottom. Keep on ice during experimental setup. Avoid prolonged exposure to room temperature or direct light.

Quality assurance: Each lot of IQCD Mouse mAb is manufactured under controlled conditions. A Certificate of Analysis (CoA) is provided with each shipment, detailing lot-specific concentration, purity assessment, and functional test results. Contact Aladdin Scientific technical support if you have questions about lot-specific performance data or require additional validation information.

Formulation note: The PBS-only formulation (no BSA, no azide as standard) means the antibody is ready for conjugation experiments. If long-term storage stability is a concern, consider adding a carrier protein (e.g., 0.1% BSA) and a preservative appropriate for your downstream application.

Frequently asked questions

What is the recommended storage condition for IQCD Mouse mAb (Ab351263)?

Store IQCD Mouse mAb at –20 °C for long-term storage. Aliquot into single-use volumes to avoid repeated freeze-thaw cycles, which can reduce antibody activity. Short-term storage at 4 °C (up to one month) is acceptable.

Is IQCD Mouse mAb suitable for direct conjugation workflows?

Yes. Because the antibody is formulated in PBS only — without BSA or other carrier proteins — it is directly compatible with fluorophore labeling, biotin conjugation, or enzyme coupling protocols. No prior buffer exchange or carrier protein removal is required.

What applications has IQCD Mouse mAb been designed for?

As a mouse monoclonal antibody, IQCD Mouse mAb is generally suited for applications such as Western blot, immunofluorescence, immunoprecipitation, and ELISA. Users should consult the product datasheet and CoA for lot-specific validated applications and recommended working dilutions.

What secondary antibody should I use with IQCD Mouse mAb?

Since IQCD Mouse mAb is raised in mouse, use an anti-mouse IgG secondary antibody appropriate for your detection method — for example, HRP-conjugated anti-mouse IgG for Western blot or fluorophore-conjugated anti-mouse IgG for immunofluorescence. Ensure the secondary antibody is validated for the species and application you are using.

How do I know which lot-specific validated applications apply to my purchase?

Each shipment of IQCD Mouse mAb (Ab351263) is accompanied by a Certificate of Analysis (CoA) that lists validated applications, working dilutions, and quality control test results specific to that lot. You can also contact Aladdin Scientific's technical support team for further assistance.

References

1. Bhatt DL et al. General principles of monoclonal antibody production and application in research. [Textbook-level reference — specific citation not available from source material]
2. UniProt Consortium. IQCD (IQ motif containing D) — Human protein entry. UniProt Knowledgebase.
3. Rhoads AR, Friedberg F. Sequence motifs for calmodulin recognition. FASEB J. 1997;11(5):331-340.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.